The Effects of Different Carbon Sources and Changes in the Growth Medium on the Shape of Cells of the Yeast Trigonopsis variabilis

Matthewson, D. K.; Barnett, James A.

doi:10.1099/00221287-83-2-427

Cited by 8 publications

(3 citation statements)

References 1 publication

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“…In S. cerevisiae, a GTPase cascade transduces spatial information to the actin cytoskeleton and induces the cell polarity (Chant 1996, Ayscough et al 1997. The ratio of triangular ceils in the culture is significantly influenced by the nitrogen source (Sasek and Becker 1969), the carbon source (Matthewson and Barnett 1974), and the presence of detergent, Tween 80 (Packer and Bersten 1977). Signal transduction pathways similar to S. cerevisiae may also work in T. variabitis to determine the polar-ity of cell growth.…”

Section: Correlation Of Cytoskeleton and Dimorphismmentioning

confidence: 96%

“…Because of its characteristics, T. variabilis has been an attractive material in terms of dimorphism of yeast (Bartnicki-Garcia and Gierz 1993). The effect of growth medium on the cellular form of T. variabilis has been extensively examined (Matthewson andBarnett 1974, Packer andBersten 1977). The behavior of organelles in triangular and ellipsoidal cells, however, has not yet been examined.…”

Section: Introductionmentioning

confidence: 99%

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Behavior of mitochondria, microtubules, and actin in the triangular yeastTrigonopsis variabilis

Miyakawa

Yanagamizu

1998

Protoplasma

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Summary. Dimorphic yeast Trigonopsis variabilis is a uniquespecies that can form either an ellipsoidal or a triangular cell depending upon nutritional conditions. This fluorescence microscopic study was intended to correlate morphological changes of mitochondria in the triangular cells with the distribution of the cytoskeleton. In addition, unique features in the behavior of the cytoskeleton were also examined during triangular cell formation. In log-phase cells stained with 4',6-diamidino-2-phenylindole, mitochondrial nucleoids appeared as a string of beads throughout the vegetative growth. The profile of mitochondria stained by 3,3'-dihexyloxacarbocyanine iodide showed a network corresponding to the fluorescence images of mitochondrial nucleoids in both mother and daughter cells. Cellcycle-dependent fragmentation of mitochondria was not discerned. As the culture reached stationary phase, a network of mitochondria gradually changed to form unique rings that were located new the angles of triangular cells. When examined by immunofluorescence microscopy with anti-tubulin antibody, microtubules were found to be well developed along the sides of ceils in the cytoplasm of T. variabilis interphase cells. Although distributions of microtubules and mitochondria are different during cell cycle as a whole, cytoplasmic microtubules frequently extended along a part of the mitochondria in budded cells, suggesting correlation of microtubules and mitochondria. Rhodamine-phalloidin staining revealed both actin patches and cables. Actin cables elongated from mother cells into the buds and showed close proximity to mitochondria, although complete overlapping of both structures was rare. Moreover, actin patches localized on the mitochondrial network at a frequency of 65%. These results suggested that actin cables and patches, as well as microtubules, participated in the distribution of mitochondria. The localization of actin patches separated towards opposite ends at a bud tip when the bud grew to medium size. The unique localization of actin patches is responsible for bi-directional growth of the bud, forming triangular cells.

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Section: Correlation Of Cytoskeleton and Dimorphismmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Behavior of mitochondria, microtubules, and actin in the triangular yeastTrigonopsis variabilis

Miyakawa

Yanagamizu

1998

Protoplasma

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“…Methionine induces the highest percentage of triangular cells and this occurs either in early-stationary phase (Sentheshanmuganathan & Nickerson, 1962) or early-exponential phase (SaSek & Becker, 1969;Matthewson & Barnett, 1974). Matthewson & Barnett (1974) also observed that T. variabilis responded to different carbon sources by a change in cell shape, which indicates that the dimorphism of Trigonopsis cannot be accounted for by a single nutritional requirement. Several reports (Rambelli, 1959;Verona & Zardetto de Toledo, 1958;Picci & Verona, 1963 ;Packer & Bersten, 1977) describe the temperature dependence of triangular cell formation in diverse growth media in batch culture.…”

Section: Introductionmentioning

confidence: 98%

A Continuous Culture Method for Obtaining 100% Triangular Cells of Trigonopsis variabilis

Packer

Bersten

1978

Journal of General Microbiology

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A history of research on yeasts 8: taxonomy

Barnett

2004

Yeast

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The Effects of Different Carbon Sources and Changes in the Growth Medium on the Shape of Cells of the Yeast Trigonopsis variabilis

Cited by 8 publications

References 1 publication

Behavior of mitochondria, microtubules, and actin in the triangular yeastTrigonopsis variabilis

Behavior of mitochondria, microtubules, and actin in the triangular yeastTrigonopsis variabilis

A Continuous Culture Method for Obtaining 100% Triangular Cells of Trigonopsis variabilis

A history of research on yeasts 8: taxonomy

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