The effects of dietary lipids on adrenergically-stimulated lipolysis in perinodal adipose tissue following prolonged activation of a single lymph node

The source of fatty acids incorporated into proliferating lymphoid cells in immune-stimulated lymph nodes

“…The morphological findings were similar to those reported previously (Mattacks et al 2002). The guinea pigs used in the present study were healthy and free from visible wounds or infection, so the popliteal lymph node would be quiescent (Smith & Morris, 1970).…”

“…Adult Bolivian guinea pigs aged 8-12 months were bred and raised on breeding-grade guinea-pig chow (FD1; Special Diet Services, Witham, UK), which contained 34 g crude lipid/kg. For 6 weeks, groups of nine animals (five females, four males) were fed on this unmodified diet, or on similar chow to which 100 g suet, sunflower oil, or fish oil was added/kg (Mattacks et al 2002). The fatty acid compositions of these diets are shown in Table 1.…”

“…The stimulated popliteal and four conspicuous mesenteric lymph nodes were dissected out and weighed. Following protocols described elsewhere (Mattacks et al 2002;, perinodal, middle and remote samples of adipose tissue were dissected from both popliteal depots, together with perinodal and remote samples from the mesentery, and the regions of the greater omentum rich in ('perinodal'), or lacking ('remote') milky spots, the patches of lymphoid tissue interspersed among adipocytes (Shimotsuma et al 1993). A sample was also taken from the perirenal depot, which in guinea pigs does not contain any lymph nodes and accounts for more than 20 % of all dissectible adipose tissue.…”

Fatty Acid Compositions of Lipids in Mesenteric Adipose Tissue and Lymphoid Cells in Patients with and Without Crohnʼs Disease and Their Therapeutic Implications

“…Animal experiments reveal qualitative similarity in the structure, chemical composition,4,9 and metabolism19,22 of all node‐containing adipose depots, with minor quantitative differences. The abnormalities of the composition of adipose tissue and lymph node lymphoid cells in the mesentery of patients with CD probably indicate similar deficiencies in the corresponding tissues elsewhere in the body.…”

“…In adult rats, the mesenteric perinodal adipocytes respond surprisingly strongly to mild, transient inflammation applied experimentally to the hind leg,21 suggesting that paracrine interactions between mesenteric adipocytes and lymphoid cells are an integral part of almost all immune responses, whether local or remote in origin. Furthermore, the fatty acid composition of dietary lipids modulates basal and noradrenalin‐stimulated lipolysis in mesenteric perinodal adipocytes, although not in the much more widely studied perirenal adipose tissue that is not closely associated with lymph nodes 22. Consequently, defects in the internal organization of mesenteric adipose tissue may contribute to atypical DC profiles23 and other immunologic irregularities that characterize CD, and account for many of its extraintestinal symptoms 24…”