The Effects of Dexamethasone on the Na,K-ATPase Activity and Pump Function of Corneal Endothelial Cells

Hatou, Shin; Yamada, Masakazu; Mochizuki, Hidetaka; Shiraishi, Atsushi; Joko, Takeshi; Nishida, Teruo

doi:10.1080/02713680902829624

Cited by 50 publications

(36 citation statements)

References 20 publications

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“…In our study, although the differences were not statistically significant, the mean CCT was higher for the CS group than for the VD group both at the fifth minute and at the 12 th h. The Na + -and K + -dependent ATPase (Na + /K + -ATPase) expressed in the basolateral membrane of corneal endothelial cells is primarily responsible for the pump function of the corneal endothelium. In two different studies of mouse corneal endothelial cells, dexamethasone was shown to increase Na + /K + -ATPase activity and pump function (12,13) . We suggest that, like the pulmonary epithelium Na + transport system, the corneal endothelial pump function may be induced by higher endogenous plasma catecholamine levels in vaginally-delivered infants, causing thinner CCT values in the VD group than in the CS group.…”

Section: Discussionmentioning

confidence: 99%

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The effects of delivery type and gender on intraocular pressure and central corneal thickness in newborns

Özkurt¹,

Balsak

et al. 2016

Arquivos Brasileiros De Oftalmologia

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Purpose: To analyze intraocular pressure (IOP) and central corneal thickness (CCT) in newborns during the first 12 h of life. Methods: Forty-three newborns born by vaginal delivery (VD) and 30 newborns born by cesarean section (CS) were evaluated. IOP and CCT were measured using Tono-Pen and handheld pachymeter, respectively, at both the 5 th minute after delivery and at the 12 th h of life. results: The mean IOP for the VD group was significantly higher than that of the CS group at both the 5 th minute and 12 th h (p=0.042 and p=0.018, respectively). In both groups, the IOP decreased by the 12 th h, but the decrease was only significant for the CS group (p=0.020). The decrease in CCT over the 12 h was significant for both groups (p<0.001). In the VD and CS groups, the IOP values of the males were significantly higher than those of the females at the fifth minute only (p=0.024 and p=0.043, respectively). No other values were significantly different between the genders. conclusions: Newborn IOP is affected by the mode of delivery and gender. A higher IOP was found in vaginally delivered newborns than in CS newborns for at least 12 h postpartum. CCT showed a significant decline within 12 h. Male newborns have significantly higher IOP values in the first minutes of life.Keywords: Cesarean section; Delivery, obstetric; Intraocular pressure; Cornea/anatomy & histology; Infant; Newborn RESUMO Objetivos: Analisar a pressão intraocular (IOP) e a espessura corneana central (CCT) em recém-nascidos durante as primeiras 12 horas de vida. Método: Quarenta e três recém-nascidos nascidos por parto vaginal (VD) e 30 recém-nascidos nascidos após cesariana (CS) foram avaliados. IOP e CCT foram medidos com Tono-Pen e Handheld Pachymeter no quinto minuto após o parto e na décima segunda hora de vida. Resultados: A média de IOP para o grupo VD foi significativamente maior do que o grupo CS tanto no quinto minuto quanto na décima segunda hora (p=0,042, p=0,018, respectivamente). Em ambos os grupos, a IOP diminuiu na décima segunda hora, mas a redução foi significativa apenas para o grupo CS (p=0,020). A diminuição da CCT nas doze horas foi significativa para ambos os grupos (p<0,001). Nos grupos VD e CS os valores de IOP dos homens foram significativamente maiores do que das mulheres

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Section: Discussionmentioning

confidence: 99%

“…Previous studies of mouse corneas have demonstrated that the pump function of the corneal endothelium can be enhanced by dexamethasone (12,13) . During VD, stress increases the plasma levels of catecholamines (14)(15)(16) .…”

Section: Introductionmentioning

confidence: 99%

The effects of delivery type and gender on intraocular pressure and central corneal thickness in newborns

Özkurt¹,

Balsak

et al. 2016

Arquivos Brasileiros De Oftalmologia

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“…Recently, a study demonstrated that dexamethasone results in increases in Na þ /K þ -ATPase pump activity in cultured corneal endothelial cells. 10 The use of topical steroids may result in an increase of the pump activity in the remaining receptors that have not been blocked by the cardenolides. This may serve to compensate for some of the lost activity caused by the inhibitory action of the cardenolides and help in the recovery process.…”

Section: Discussionmentioning

confidence: 99%

Corneal endothelial toxicity secondary to Asclepias fruticosa

Amiran

Lang

Yeung

2011

Eye

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“…However, GRs also participate in the regulation of Na + ,K + -ATPase (Hatou, et al, 2009;Derfoul. et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Distribution of glucocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase isoforms in the rat inner ear

2011

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Abstract11β-hydroxysteroid dehydrogenase (11β-HSD) is an enzyme complex responsible for the conversion of hormonally active cortisol to inactive cortisone, and two isoforms of the enzyme (11β-HSD1 and 11β-HSD2) have been cloned and characterized. An immunohistochemical study was performed to determine the precise distribution of glucocorticoid receptors (GRs) and the isoforms of 11β-HSD in the rat (postnatal day 1, 4, 10, and adult). Immunoreactivity of GRs was detected in the stria vascularis (SV), the outer hair cells (OHCs), the inner hair cells (IHCs), the spiral ligament (SLig), the spiral limbus (SLib), the spiral ganglion cells (SGCs), Reissner's membrane (RM), the cochlear nerve (CN), the vestibular hair cells (VHCs), the dark cells (DCs), and the vestibular nerve (VN) in the rats. Immunostaining of 11β-HSD1 was observed in almost all the tissues in the cochlea and the vestibule except SLig, SLib, SGCs, CN, VHCs, and VN during all developmental stages, whereas, immunoreativity of 11β-HSD2 was not detected in any of the inner ear tissues. A polymerase chain reaction (PCR) study was also performed on GRs, 11β-HSD1, and 11β-HSD2 in the OC, SV and vestibule of the postnatal rats, and revealed that mRNAs were detected in all those and tissues in all the developmental days of postnatal days 1, 4, and 10. This data indicates that expression of GRs and 11β-HSD isoforms in the inner ear is tissue and age-specific, and that different local steroid regulation by GRs and the isoforms of 11β-HSD is present in each part of the inner ear.

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The Effects of Dexamethasone on the Na,K-ATPase Activity and Pump Function of Corneal Endothelial Cells

Abstract: Our results suggest that dexamethasone stimulates Na,K-ATPase activity in mouse corneal endothelial cells. The effect of dexamethasone activation in these cells is mediated by Na,K-ATPase synthesis and increase in an enzymatic activity by dephosphorylation of Na,K-ATPase alpha(1)-subunits.

Cited by 50 publications

References 20 publications

The effects of delivery type and gender on intraocular pressure and central corneal thickness in newborns

The effects of delivery type and gender on intraocular pressure and central corneal thickness in newborns

Corneal endothelial toxicity secondary to Asclepias fruticosa

Distribution of glucocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase isoforms in the rat inner ear

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