The effects of cytoxan on the chromosomes of mouse bone marrow

Datta, Preeta; Schleiermacher, E.

doi:10.1016/0027-5107(69)90080-3

Cited by 38 publications

(4 citation statements)

References 8 publications

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“…This recorded data was in complete agreement with earlier reports (Datta and Schliermacher, 1969;Rohrborn and Basler, 1977;Machemer and Lorke, 1978;Iarc, 1981;Valadares et al, 2007;Barekati et al, 2008;Tripathi and Jena, 2009). Also, in the present study, the infection of mice with the three different concentrations of rabies virus CVS showed a significant increase in both structural and numerical chromosomal aberrations and in micronuclei as well, while the mitotic activity of mice bone marrow cells were decreased.…”

Section: Discussionsupporting

confidence: 93%

Protective role of interferon against cytotoxcicity induced by rabies virus in mice

Tohamy¹,

Fahmy²,

Dkhil³

et al. 2010

Afr. J. Biotechnol.

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Rabies remains an important public health problem in the world due to uncontrolled enzootic rabies, lack of safe efficient vaccines and poor information on the risk of contracting rabies post animal exposure. The lethality and mutagenic potential of challenge virus standard (CVS) was evaluated in mice. Mice were intracerebrally infected (MIC) with low, medium and high viral LD 50 (MICLD 50 ). Mice were subjected to immunomodulation using interferon (IFNα-2a) pre-infection. The infected groups pretreated with IFN-α 2a showed a higher survival rate than the infected group. Statistically significant increase in structural and numerical chromosomal aberrations and a decreased mitotic activity of mice bone marrow cells was observed post infection. Pretreatment of the infected groups with IFN α-2a showed a marked and significant reduction of these cytogenetic changes. The increased survival rate and reduced cytogenetic changes suggest that protection induced by interferon against rabies virus activity could be, at least partially, attributable to blockage of the replication of CVS strain of rabies virus. It could be concluded that interferon can be used as an immune enhancer to the application of vaccine administration.

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Section: Discussionsupporting

confidence: 93%

Protective role of interferon against cytotoxcicity induced by rabies virus in mice

Tohamy¹,

Fahmy²,

Dkhil³

et al. 2010

Afr. J. Biotechnol.

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“…The chromosome aberrations had disappeared 72 h after therapy. The temporal ralationship between the administration of a cytostatic agent and the appearance of structural changes of chromosomes has only been studied as regards cyclophosphamide (Datta & Schleiermacher 1969), and rubidomycin (Krogh Jensen & Philip 1971). Both agents seem to behave like melphalan.…”

Section: Discussionmentioning

confidence: 99%

The Induction of Chromosome Abnormalities by Melphalan in Rat Bone Marrow Cells

Wantzin

Jensen

1973

Scandinavian Journal of Haematology

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The cytogenetic effect of melphalan was studied in bone marrow cells of Wistar rats in vivo. Melphalan produces structural aberrations of the chromatid and chromosome type, whereas no numerical aberrations were seen. The proportion of abnormal metaphases varied with the period that had elapsed since the administration of melphalan. Repeated large doses of melphalan were not able to produce permanent damage to the chromosome complement.

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“…In mice, DATTA and SCHLEIERMACHER [12] found an optimum effect after 24 hours. According to our own in vivo cytogenetic studies (bone marrow) in mice (NMRI-EMD-SPF) with 6-mercaptopurine, a maximum aberration rate of 55.1 ~o was found 48 hours after the administration.…”

Section: Optimum Time Of Examinationmentioning

confidence: 95%

Critique of in vivo cytogenetic test systems

Frohberg

1973

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Experiments in laboratory animals in general are influenced by a number of factors that have to be taken into account in considering the results of cytogenetic studies. The points that are supposed to be pertinent are discussed in the following. Selection of speciesThe animals used in cytogenetic tests should be easy to breed, and their chromosomes should be easily identifiable. Beagle dogs, a species widely used in toxicological work, have 72 chromosomes that are difficult to analyze. The 40 chromosomes of the mouse are achrocentric and very similar in size. However, this species has the advantage that a large amount of genetic information is available.Rats, that are also widely used in toxicology seem to be more suitable animals for cytogenetic work than mice. Although the chromosome number of 42 is rather high the individual chromosomes are easily identifiable because of their different size and shape.The Chinese hamster is considered to be the most suitable animal for cytogenetic studies. This species has 22 chromosomes, all of which are morphologically easily identifiable. Species differencesAccording to cytogenetic investigations by R6HRBORN et al. [27] mice proved to be more sensitive to cyclophosphamide than rats, and these were more responsive than Chinese hamsters.

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The effects of cytoxan on the chromosomes of mouse bone marrow

Cited by 38 publications

References 8 publications

Protective role of interferon against cytotoxcicity induced by rabies virus in mice

Protective role of interferon against cytotoxcicity induced by rabies virus in mice

The Induction of Chromosome Abnormalities by Melphalan in Rat Bone Marrow Cells

Critique of in vivo cytogenetic test systems

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