The effects of cysteine addition during <i>in vitro</i> maturation on the developmental competence, ROS, GSH and apoptosis level of bovine oocytes exposed to heat stress

Nabenishi, Hisashi; Ohta, Hiroshi; Nishimoto, Tadashi; Morita, Tetsuo; Ashizawa, Koji; Tsuzuki, Yasuhiro

doi:10.1017/s0967199411000220

Cited by 93 publications

(93 citation statements)

References 51 publications

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“…In some instances glutathione content is lower in heat-stressed oocytes (Wang et al 2009, Nabenishi et al 2011. In our laboratory, heat-stressed oocytes may contain w12% less glutathione than nonheat-stressed counterparts (J L Edwards & R R Payton 2011, unpublished observations).…”

Section: Discussionmentioning

confidence: 85%

Disparate consequences of heat stress exposure during meiotic maturation: embryo development after chemical activation vs fertilization of bovine oocytes

Rispoli¹,

Lawrence²,

Payton³

et al. 2011

Reproduction

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Consequences of heat stress exposure during the first 12 h of meiotic maturation differed depending on how and when bovine oocytes were activated. If heat-stressed oocytes underwent IVF at w24 h, blastocyst development was less than for respective controls and similar to that obtained for nonheat-stressed oocytes undergoing IVF at 30 h (i.e. slightly aged). In contrast, if heat-stressed oocytes underwent chemical activation with ionomycin/6-dimethylaminopurine at 24 h, blastocyst development was not only higher than respective controls, but also equivalent to development obtained after activation of nonheat-stressed oocytes at 30 h. Developmental differences in chemically activated vs IVF-derived embryos were not related to fertilization failure or gross alterations in cytoskeletal components. Rather, ionomycin-induced calcium release and MAP kinase activity were less in heat-stressed oocytes. While underlying mechanisms are multifactorial, ability to obtain equivalent or higher development after parthenogenetic activation demonstrates that oocytes experiencing heat stress during the first 12 h of meiotic maturation have the necessary components to develop to the blastocyst stage, but fail to do so after fertilization.

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Section: Discussionmentioning

confidence: 85%

Disparate consequences of heat stress exposure during meiotic maturation: embryo development after chemical activation vs fertilization of bovine oocytes

Rispoli¹,

Lawrence²,

Payton³

et al. 2011

Reproduction

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“…This result indicated that higher concentrations of cysteine and cystine do not improve embryonic development, possibly due to high cystine concentrations disrupting the redox equilibrium in cells (18). However, a previous bovine study suggested that adding 1.2 mM of cysteine to IVM media inhibits the production of ROS in oocytes and the apoptosis of cumulus cells (19). Different culture media, and the combination of cysteine and cystine may affect the concentrations used.…”

Section: Discussionmentioning

confidence: 93%

Using cysteine/cystine to overcome oxidative stress in goat oocytes and embryos cultured in vitro

Zhou

Jia

Zhang

et al. 2016

Molecular Medicine Reports

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Abstract. Assisted reproductive techniques expose gametes to excessive concentrations of reactive oxygen species. The present study aimed to evaluate the effects of oxidative stress on apoptosis in goat oocytes and embryonic development. The results demonstrated that the addition of 100 µM hydrogen peroxide (H 2 O 2 ) into media produces an oxidative environment during oocyte maturation. The number of cumulus cells positive for terminal deoxynucleotidyl transferase UTP nick end labeling, and the activity of caspase 3 in mature oocytes were increased, compared with the control group (P<0.05). In addition, the expression levels of mitochondrial regulators, including peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1α) and nuclear respiratory factor-1 (NRF-1) were increased in the oxidative oocytes, compared with those in the control group (P<0.05). The ratio of the proapoptotic gene, B cell lymphoma (Bcl-2)-associated X pr otein (BAX), to the anti-apoptotic gene, BCL-2, was higher in the H 2 O 2 group, compared with the control group (P<0.05). To overcome oxidative stress in oocytes and embryos cultured in vitro, 200 µM cysteine and 200 µM cystine were added to the media, thereby increasing the concentration of intracellular glutathione (GSH) and assisting in maintaining the redox state of the cells. In conclusion, cysteine and cystine reduced the oxygen tension caused by H 2 O 2 , thereby providing a novel strategy for optimizing in vitro embryonic development systems.

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“…When cultured with follicular hemisections, progression to MII was enhanced using progesterone concentrations %100 ng; at concentrations R1000 ng, meiotic progression was less than optimal. Depending on the extent to which heat-induced increases in the production of progesterone affect meiotic progression, it would be interesting to evaluate progesterone levels in instances where the consequences of heat stress are severe enough to inhibit meiotic progression (Roth & Hansen 2005, MayaSoriano et al 2012, Nabenishi et al 2012.…”

Section: Discussionmentioning

confidence: 99%

Heat stress effects on the cumulus cells surrounding the bovine oocyte during maturation: altered matrix metallopeptidase 9 and progesterone production

Rispoli¹,

Payton²,

Gondro³

et al. 2013

Reproduction

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When the effects of heat stress are detrimental during maturation, cumulus cells are intimately associated with the oocyte. To determine the extent to which heat stress affects these cells, in this study, transcriptome profiles of the cumulus that surrounded control and heat-stressed oocytes (41 8C during the first 12 h only and then shifted back to 38.5 8C) during in vitro maturation (IVM) were compared using Affymetrix bovine microarrays. The comparison of cumulus-derived profiles revealed a number of transcripts whose levels were increased (nZ11) or decreased (nZ13) R twofold after heat stress exposure (P!0.01), sufficient to reduce the development of blastocysts by 46.4%. In a separate study, quantitative PCR (qPCR) was used to confirm heat-induced differences in the relative abundances of the transcripts of five different genes (caveolin 1, matrix metallopeptidase 9, FSH receptor, Indian hedgehog homolog, and inducible nitric oxide synthase). Heat stress exposure resulted in O1.7-fold decrease in the protein levels of latent matrix metallopeptidase 9 (proMMP9). Heat-induced reductions in transcript levels were noted at 6 h IVM with reductions in proMMP9 protein levels at 18 h IVM (PZ0.0002). Independent of temperature, proMMP9 levels at 24 h IVM were positively correlated with the development rate of blastocysts (R 2 Z0.36; PZ0.002). The production of progesterone increased during maturation; heat-induced increases were evident by 12 h IVM (PZ0.002). Both MMP9 and progesterone are associated with the developmental competence of the oocyte; thus, it seems plausible for some of the negative consequences of heat stress on the cumulus-oocyte complex to be mediated through heat-induced perturbations occurring in the surrounding cumulus.

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The effects of cysteine addition during in vitro maturation on the developmental competence, ROS, GSH and apoptosis level of bovine oocytes exposed to heat stress

Cited by 93 publications

References 51 publications

Disparate consequences of heat stress exposure during meiotic maturation: embryo development after chemical activation vs fertilization of bovine oocytes

Disparate consequences of heat stress exposure during meiotic maturation: embryo development after chemical activation vs fertilization of bovine oocytes

Using cysteine/cystine to overcome oxidative stress in goat oocytes and embryos cultured in vitro

Heat stress effects on the cumulus cells surrounding the bovine oocyte during maturation: altered matrix metallopeptidase 9 and progesterone production

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