The Effects of Cortisol on Heat Shock Protein 70 Levels in Two Fish Species

Basu, Niladri; Nakano, T.; Grau, E. Gordon; Iwama, George K.

doi:10.1006/gcen.2001.7688

Cited by 166 publications

(115 citation statements)

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“…Moreover, there is increasing evidence linking changes in stress hormones to changes in stress proteins (e.g. Paroo & Noble 1999;Basu et al 2001;Currie et al 2008), demonstrating an integration of the physiological and cellular stress responses. Given the profound effect of behaviour on both physiological and molecular responses, our goal was to determine the effects of social stress in rainbow trout by examining the expression of Hsps.…”

Section: Introductionmentioning

confidence: 99%

Agonistic encounters and cellular angst: social interactions induce heat shock proteins in juvenile salmonid fish

et al. 2009

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Juvenile salmonid fish readily form dominance hierarchies when faced with limited resources. While these social interactions may result in profound behavioural and physiological stress, it is unknown if this social stress is evident at the level of the cellular stress response-specifically, the induction of stress or heat shock proteins (Hsps). Thus, the goal of our study was to determine if Hsps are induced during hierarchy formation in juvenile rainbow trout (Oncorhynchus mykiss). To this end, we measured levels of three Hsps, Hsp70, Hsc (heat shock cognate)70 and Hsp90 in the white muscle, liver and brain of trout that had been interacting for 36 h, 72 h or 6 days. Our data indicate that Hsps are induced in both dominant and subordinate fish in a time-and tissue-specific manner. In further mechanistic experiments on fasted and cortisol-treated fish, we demonstrated that high plasma cortisol does not affect Hsp induction in trout white muscle or liver, but both conditions may be part of the mechanism for Hsp induction with social stress in the brain. We conclude that the behavioural and physiological stress experienced by juvenile rainbow trout in dominance hierarchies can be extended to the induction of Hsps.

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Section: Introductionmentioning

confidence: 99%

Agonistic encounters and cellular angst: social interactions induce heat shock proteins in juvenile salmonid fish

et al. 2009

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“…Therefore, it may be possible that the 10 o C increase in temperature, which made the fish return to their normal temperature range, was not enough to elicit a heat shock response. similarly, tilapia (Oreochromis mossambicus) did not elicit a heat shock response when subjected to an increase in water temperature within their normal temperature range (Basu et al, 2001).…”

Section: Discussionmentioning

confidence: 96%

“…Concomitant increase cortisol and HsP70 levels have been documented in rainbow trout (Oncorhynchus mykiss) exposed to heat shock (Basu et al, 2001). The same authors demonstrated that tilapia (Oreochromis mossambicus) subjected to heat shock only elicited the cortisol response.…”

Section: Introductionmentioning

confidence: 89%

“…Plasma cortisol levels (ng/ml) were measured in duplicate using an enzyme-linked immunosorbent assay kit from neogen Corporation (Lansing, MI, usA) (Basu et al, 2001). The absorbance was read on a Molecular Devices spectraMax 340PC microplate reader (λ = 450 nm).…”

Section: Plasma Cortisol and Glucose Levelsmentioning

confidence: 99%

“…Prestained molecular weight markers (Invitrogen life technologies, Carlsbad, CA, usA) and a positive control (rainbow trout gonad (heat shocked) cell lysates -stressgen Biotechnologies Corp., Victoria, BC, Canada) were loaded to every gel. following electrophoretic separation, the proteins were transferred onto nitrocellulose membranes for immunoblotting (Basu et al 2001). Membranes were then incubated for 35 min in the primary antibody monoclonal anti-heat shock protein 70 antibody (produced in mouse 1:10000 dilution (H5147 sigma, saint Louis, MI, usA)).…”

Section: Hsp70 Levels -Sds-page and Immunoblottingmentioning

confidence: 99%

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Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

et al. 2008

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The present work evaluated several aspects of the generalized stress response [endocrine (cortisol), metabolic (glucose), hematologic (hematocrit and hemoglobin) and cellular (HsP70)] in the Amazonian warm-water fish matrinxã (Brycon amazonicus) subjected to an acute cold shock. This species farming has been done in south America, and growth and feed conversion rates have been interesting. However, in subtropical areas of Brazil, where the water temperature can rapidly change, high rates of matrinxã mortality have been associated with abrupt decrease in the water temperature. Thus, we subjected matrinxã to a sudden cold shock by transferring the fish directly to tanks in which the water temperature was 10 o C below the initial conditions (cold shock from 28°C to 18 o C). After 1h the fish were returned to the original tanks (28ºC). The handling associated with tank transfer was also imposed on control groups (not exposed to cold shock). while exposure to cold shock did not alter the measured physiological conditions within 1h, fish returned to the ambient condition (water at 28º C) significantly increased plasma cortisol and glucose levels. exposure to cold shock and return to the warm water did not affect HsP70 levels. The increased plasma cortisol and glucose levels after returning the fish to warm water suggest that matrinxã requires cortisol and glucose for adaptation to increased temperature.Key words: matrinxã, cold shock, stress, HsP70.Respostas fisiológicas ao estresse do peixe de águas tépidas matrinxã (Brycon amazonicus) submetido à queda brusca de temperatura resumo o presente trabalho avaliou as principais respostas fisiológicas e celulares [endócrino (cortisol), metabólico (glicose), hematológico (hematócrito and hemoglobina) e celular (HsP70)] ao estresse de um peixe de águas tépidas, o matrinxã (Brycon amazonicus), quando submetido a um choque térmico frio abrupto. essa espécie vem sendo amplamente cultivada na América do sul por apresentar excelentes índices zootécnicos de crescimento e conversão alimentar. entretanto, os produtores rurais encontram limitações no manejo do matrinxã, quando criado em regiões mais frias que sua região de origem, a Bacia Amazônica. Assim, o matrinxã foi submetido a um choque frio através da transferência direta dos peixes para tanques com água fria a 18°C. Após 1h, esses peixes retornaram a suas caixas de origem a 28°C. o manuseio de peixes necessário para conduzir o choque térmico experimental foi também imposto aos grupos controle, sendo, entretanto, evitada a água fria. o matrinxã demonstrou claros sinais de estresse fisiológico durante os procedimentos experimentais. Porém, essas respostas não foram associadas ao choque frio, mas sim ao choque quente por ocasião da volta dos peixes para as caixas de origem. As respostas primárias e secundárias de estresse foram evidentes através das análises plasmáticas de cortisol e glicose. Já o hematócrito, a hemoglobina e as expressões da proteína de estresse, HsP70, não foram afetadas. nossos resultados sustentam qu...

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Stress in Fish and Application of Carotenoid for Aquafeed as an Antistress Supplement

Nakano

2020

Encyclopedia of Marine Biotechnology

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The Effects of Cortisol on Heat Shock Protein 70 Levels in Two Fish Species

Cited by 166 publications

References 28 publications

Agonistic encounters and cellular angst: social interactions induce heat shock proteins in juvenile salmonid fish

Agonistic encounters and cellular angst: social interactions induce heat shock proteins in juvenile salmonid fish

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

Stress in Fish and Application of Carotenoid for Aquafeed as an Antistress Supplement

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