The effects of cooling and vitrification of embryos from mares treated with equine follicle-stimulating hormone on pregnancy rates after nonsurgical transfer

Comparison of different cryopreservation methods for horse and donkey embryos

“…A low pregnancy rate (22%) was obtained when horse embryos vitrified in Fibreplugs were transferred to recipient mares. This percentage was broadly similar to the pregnancy rates described by some previous authors [6,27], but lower than the values of around 45-75% described by others [11,28]. A recent vitrification study [29] conducted on small embryos (<300 lm) using commercial kits and straws reported pregnancy rates similar to those obtained by slow freezing (Supplementary Item 1).…”

“…This percentage was broadly similar to the pregnancy rates described by some previous authors [6,27], but lower than the values of around 45-75% described by others [11,28]. This percentage was broadly similar to the pregnancy rates described by some previous authors [6,27], but lower than the values of around 45-75% described by others [11,28].…”

Evaluation of Capsule Permeability in the Equine Blastocyst

“…Glycerol is commonly used in equine embryo cryopreservation at different concentrations depending on freezing protocol. For slow freezing, 1.4 M is used [22,23], and for vitrification, 3.4 M is normally used [24,25].…”

Superovulation in Mares

“…They reported similar development in culture between embryos recovered from superovulated mares and single-ovulating mares. Recently, Hudson and colleagues [28] reported on the effects of cooling and vitrification of embryos from mares treated with eFSH on pregnancy rates after nonsurgical transfer. Embryos were collected from 38 eFSH-treated mares 6.5 days after ovulation or, if ovulations were asynchronous, 8 days after administration of hCG.…”