The Effects of Combined Gentamicin and White Noise on the Spiral Organ of Young Guinea Pigs

Dodson, H.C.; Bannister, L. H.; Douek, Ellis

doi:10.3109/00016488209128905

Cited by 28 publications

(10 citation statements)

References 16 publications

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“…The appearance of cytoplasmic vacuoles could be due to dilatation and fusion of subsurface cisternae which would result in wrinkling of the plasma membrane and distortion of the shape of the hair cell body. In support with this suggestion, the subsurface cisternae are supposed to maintain the cylindrical shape of OHC by stiffening its surface [18]. …”

Section: Discussionmentioning

confidence: 98%

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Effect of nicotine on the structure of cochlea of guinea pigs

et al. 2014

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Smoking has been positively associated with hearing loss in human. However, its effect on the cochlea has not been previously evaluated. Aim of work is to investigate the effect of nicotine, which is the primary pharmacological component of tobacco, on the structure of the cochlea of adult male guinea pigs. Fifteen male guinea pigs were classified into two groups: group I (control) and group II (nicotine treated group). Group II was further subdivided into two subgroups; IIA and IIB according to the dose of nicotine (3 mg/kg and 6 mg/kg, respectively). The cochlea was harvested and processed for light microscopy, transmission electron microscopy and scanning electron microscopy. Nicotine administration induced damage of outer hair cells which were distorted in shape with vacuolated cytoplasm and heterochromatic nuclei. Topography revealed damage of the stereocilia which included disorganization, bent and limp or complete loss and expansion of the surrounding supporting cells. These changes were more pronounced in the basal turn of the cochlea and mainly involved the outer hair cells. High dose induced more damage and resulted in protrusion of the apical poles of hair cells (blebing), particularly the outer two rows. Nicotine is proved to be harmful to the cells of the cochlea, particularly the outer hair cells of the basal turn. High doses induce blebing of hair cells.

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Section: Discussionmentioning

confidence: 98%

“…Actin depolymerization of the stereocilia was hypothesized to account for hearing loss [24]. The malformation in the sensory hair cells and their stereocilia would consequently lead to clinical auditory impairment as recorded in human [18], in students [25] and in experimental animals as guinea pigs [24]. …”

Section: Discussionmentioning

confidence: 99%

Effect of nicotine on the structure of cochlea of guinea pigs

et al. 2014

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“…This effect has, therefore, been predicted for humans and anticipated to be enhanced in human infants (see Li and Steyger 2009). The prediction of amplified synergistic interactions early in life has found some support in animal studies (Dodson et al 1982) but, to date, no direct support in humans (see citations in Li and Steyger 2009). The robustness of the protection we find in young CBA/J mice may mean that at least for low KM doses, the true nature of KM/noise interactions during the early window is just the opposite of predicted outcomes.…”

Section: Kanamycin In the Early Vulnerability Windowmentioning

confidence: 99%

Protection against Noise-Induced Hearing Loss in Young CBA/J Mice by Low-Dose Kanamycin

Fernandez

Ohlemiller

Gagnon

et al. 2010

JARO

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Animal studies indicate that a combination of kanamycin (KM) and noise produces a synergistic effect, whereby the threshold shift from the combination is greater than the sum of the shifts caused by either agent alone. Most such studies have focused on adult animals, and it has remained unclear whether younger, presumably more susceptible, animals show an even greater synergistic effect. The present study tested the hypothesis that young CBA/J mice receiving a low dose of KM (300 mg/kg, 2×/day, s.c.) from 20 to 30 days post-gestational age followed by brief noise exposure (110 dB SPL; 4-45 kHz, 30 s) would show greater noise-induced permanent threshold shifts (NIPTS) than mice receiving either treatment alone. Noise exposure produced 30-40 dB of NIPTS and moderate hair cell loss in young saline-treated mice. KM alone at this dose had no effect on thresholds. Surprisingly, mice receiving KM plus noise were protected from NIPTS, showing ABR thresholds not significantly different from unexposed controls. Mice receiving KM prior to noise exposure also showed significantly less outer hair cell loss than saline-treated mice. Additional experiments indicated protection by KM when the noise was applied either 24 or 48 h after the last KM injection. Our results demonstrate a powerful protective effect of subchronic low-dose kanamycin against NIPTS in young CBA/J mice. Repeated kanamycin exposure may establish a preconditioned protective state, the molecular bases of which remain to be determined.

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“…Today several environmental factors are known to cause an increased risk of hearing loss. They include coexposure to noise and chemicals (organic solvents, heavy metals) (Sliwinska-Kowalska et al, 2005), ototoxic agents (aminoglycosides) (Dayal et al, 1971;Brown et al, 1980;Dodson et al, 1982), heat (Pekkarinen, 1995), noise impulsiveness and vibrations . Associations have also been observed between several individual factors and NIHL, including smoking (Nomura et al, 2005), elevated blood pressure (Starck et al, 1999), cholesterol levels (Toppila et al, 2001), skin pigmentation (Barrenäs, 1998), gender (Berger et al, 1978) and age (for a review see Borg et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Gene Polymorphisms Associated with K⁺ Ion Circulation in the Inner Ear of Patients Susceptible and Resistant to Noise‐induced Hearing Loss

Pawełczyk

Laer

Fransén

et al. 2009

Annals of Human Genetics

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SummaryNoise-induced hearing loss (NIHL) is one of the leading occupational health risks in industrialized countries. It results from an interaction between environmental and genetic factors, however the nature of the genetic factors contributing to NIHL has not yet been clarified. Here, we investigated whether genetic variations in 10 genes putatively involved in the potassium recycling pathway in the inner ear may influence susceptibility to noise. 99 SNPs were genotyped in Polish noise-exposed workers, categorized into susceptible and resistant subjects. The most interesting results were obtained for KCNE1 and KCNQ4 as we replicated associations that were previously reported in a Swedish sample set, hence confirming that they are NIHL susceptibility genes. Additionally we report significant associations in GJB1, GJB2, GJB4, KCNJ10 and KCNQ1, however due to the lack of replication in the Swedish sample set, these results should be seen as suggestive.

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The Effects of Combined Gentamicin and White Noise on the Spiral Organ of Young Guinea Pigs

Cited by 28 publications

References 16 publications

Effect of nicotine on the structure of cochlea of guinea pigs

Effect of nicotine on the structure of cochlea of guinea pigs

Protection against Noise-Induced Hearing Loss in Young CBA/J Mice by Low-Dose Kanamycin

Analysis of Gene Polymorphisms Associated with K⁺ Ion Circulation in the Inner Ear of Patients Susceptible and Resistant to Noise‐induced Hearing Loss

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The Effects of Combined Gentamicin and White Noise on the Spiral Organ of Young Guinea Pigs

Cited by 28 publications

References 16 publications

Effect of nicotine on the structure of cochlea of guinea pigs

Effect of nicotine on the structure of cochlea of guinea pigs

Protection against Noise-Induced Hearing Loss in Young CBA/J Mice by Low-Dose Kanamycin

Analysis of Gene Polymorphisms Associated with K+ Ion Circulation in the Inner Ear of Patients Susceptible and Resistant to Noise‐induced Hearing Loss

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Analysis of Gene Polymorphisms Associated with K⁺ Ion Circulation in the Inner Ear of Patients Susceptible and Resistant to Noise‐induced Hearing Loss