The effects of co-culture with fibroblasts and angiogenic growth factors on microvascular maturation and multi-cellular lumen formation in HUVEC-oriented polymer fibre constructs

Sukmana, Irza; Vermette, Patrick

doi:10.1016/j.biomaterials.2010.02.076

Cited by 35 publications

(35 citation statements)

References 39 publications

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“…Moreover, in 100-μm microgrooves, it was frequently observed that cell branches connected with each other and formed networks. As such networks would subsequently lead to the formation of lumens in a vascularization process, the present finding that the formation of multicellular network was predominant in 100-μm microgrooves may suggest that 100-μm microgrooves were a preferred condition among the four types, which is consistent with the findings of previous studies [5,10]. Therefore, this agreement proves that the microgroove structure can be an alternative experimental model to polymer fibers in the study of microvascularization.…”

Section: Discussionsupporting

confidence: 92%

“…PET fibers which had been used as a geometrical guidance to endothelial cells for microvascularization in previous studies [5,10], were replaced with a micrometer-scale structure, microgrooves. Using MEMS techniques, microgrooves with different sizes (groove width) can be patterned in a small area.…”

Section: Experimental Modelmentioning

confidence: 99%

“…2(b)). The 100-μm-wide spacing mimicked the diameter of PET fibers used in the studies of Sukmana and Vermette [5,10].…”

Section: Experimental Modelmentioning

confidence: 99%

“…It was reported that VEGF has the ability of changing EC shape during vascular lumen formation [9]. In another study of Sukmana and Vermette, it was demonstrated that VEGF has a promotional effect on tube-like structure formation between adjacent PET fibers and the effect was a dose-dependent with an optimal VEGF concentration of 2 ng/ml [10].…”

Section: Introductionmentioning

confidence: 97%

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Development of fibrin gel-microgroove model for microvascularization by endothelial cells

et al. 2015

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This study was performed to develop a new experimental device with a fibrin gel-microgroove structure for study of microvascularization by endothelial cells (ECs). The effects of the width of microgrooves, initial cell seeding density and a supplementation of vascular endothelial growth factor (VEGF) on in vitro microvasculaization of ECs were examined. ECs were cultured in a fibrin gel formed on a polydimethylsiloxane microgroove substrate, with the microgroove width of 50, 100, 150 and 200 μm. ECs were elongated and sprouted within the gel in all the four types of microgrooves. In addition, multicellular network by connected cell branches were frequently observed in 100-μm microgrooves. Both high initial cell density and VEGF demonstrated significant promotional effects on morphology changes. The findings indicate that microgroove structure serves as a geometrical constraint for ECs, with a promotional effect on angiogenic responses of ECs, and thus, it can be used as an experimental model in the study of in vitro vascularization.

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Section: Discussionsupporting

confidence: 92%

Section: Experimental Modelmentioning

confidence: 99%

“…2(b)). The 100-μm-wide spacing mimicked the diameter of PET fibers used in the studies of Sukmana and Vermette [5,10].…”

Section: Experimental Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Development of fibrin gel-microgroove model for microvascularization by endothelial cells

et al. 2015

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“…Using cocultures of HFFs and HUVECs, several groups have demonstrated lumen formation, even after 10 days of culture. 12,29,30 Additionally, they have shown that a connection of the preformed network to the host vasculature using a coculture system of fibroblasts and HUVECs is possible after transplantation in vivo. 5 After 9 days, we could observe the initial signs of luminal development.…”

Section: Discussionmentioning

confidence: 98%

Biofunctionalized Microfiber-Assisted Formation of Intrinsic Three-Dimensional Capillary-Like Structures

Weinandy

Laffar

Unger

et al. 2014

Tissue Engineering Part A

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Vascularized Lung Cancer Model for Evaluating the Promoted Transport of Anticancer Drugs and Immune Cells in an Engineered Tumor Microenvironment

Kim

Hwang

Seo

et al. 2022

Adv Healthcare Materials

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The tumor microenvironment (TME) is the environment around the tumor, including blood vessels, immune cells, fibroblasts, signaling molecules, and the extracellular matrix (ECM). Owing to its component interactions, the TME influences tumor growth and drug delivery in a highly complex manner. Although several vascularized cancer models are developed to mimic the TME in vitro, these models cannot comprehensively reflect blood vessel-tumor spheroid interactions. Here, a method for inducing controlled tumor angiogenesis by engineering the microenvironment is presented. The interstitial flow direction regulates the direction of capillary sprouting, showing that angiogenesis occurs in the opposite direction of flow, while the existence of lung fibroblasts affects the continuity and lumen formation of sprouted capillaries. The vascularized tumor model shows enhanced delivery of anticancer drugs and immune cells to the tumor spheroids because of the perfusable vascular networks. The possibility of capillary embolism using anticancer drug-conjugated liquid metal nanoparticles is investigated using the vascularized tumor model. This vascularized tumor platform can aid in the development of effective anticancer drugs and cancer immunotherapy.

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The effects of co-culture with fibroblasts and angiogenic growth factors on microvascular maturation and multi-cellular lumen formation in HUVEC-oriented polymer fibre constructs

Cited by 35 publications

References 39 publications

Development of fibrin gel-microgroove model for microvascularization by endothelial cells

Development of fibrin gel-microgroove model for microvascularization by endothelial cells

Biofunctionalized Microfiber-Assisted Formation of Intrinsic Three-Dimensional Capillary-Like Structures

Vascularized Lung Cancer Model for Evaluating the Promoted Transport of Anticancer Drugs and Immune Cells in an Engineered Tumor Microenvironment

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