The effects of chronic radiation of gamma ray on protein expression and oxidative stress in<i>Brachypodium distachyon</i>

Kim, Dae Yeon; Hong, Min Jeong; Park, Cheong Sool; Seo, Yong Weon

doi:10.3109/09553002.2015.1012307

Cited by 25 publications

(15 citation statements)

References 81 publications

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“…Furthermore, tests performed with freshwater molluscs (Bellamya bengalensis) have shown that the increase in global temperature also induces an increase in oxidative stress, since the higher temperature, metabolic rate is increased with increased O2 consumption and oxygen-radicals generation in the tissues [20]. These results were similar to the high levels of TBARS observed in experiments with Biomphalaria glabrata, where it was verified that irradiation deregulated the mechanisms of antioxidant compensation present in the organism [21].…”

Section: Resultssupporting

confidence: 59%

Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation

et al. 2018

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Ionizing radiation can cause biological changes in different organisms such as mollusks from Biomphalaria glabrata species, in which alterations could be observed in the reproductive system of the specimens, prejudicing fertility and fecundity. As the changes may occur due to the lipid peroxidation caused by the action of free radicals on the gonads, the objective of this work was to evaluate the oxidative damage caused by the exposure of B. glabrata mollusks to different doses of 60 Co gamma radiation. In addition, efforts were carried out to standardize a sensitive and low-cost technique for detecting negative effects caused by high doses of ionizing radiation. For this, each mollusk group (n = 10) was submitted to 0 (control), 10, 15, 20 and 25 Gy (gammacell 60 Co, dose rate 3.53 kGy/h). The TBARS method was applied for the quantification of lipid peroxidation of the gonads of the mollusks after 24 and 48 h. ANOVA, followed by the mean comparison (Tukey) at the 5% of significance level (p<0.05), indicated high concentrations of TBARS in the gonads after 24 h. Otherwise, after 48 h, differences for TBARS concentrations were not significant, determining that the action of free radicals from ionizing radiation on cell membranes mainly occurred within 24 h after irradiation. Therefore, the TBARS assay could be applied for detecting oxidative stress caused by short exposure of B. glabrata to ionizing radiation.

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Section: Resultssupporting

confidence: 59%

Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation

et al. 2018

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“…Total protein content was quantified using the Bradford assay, using BSA as a standard (Bradford 1976). The activity of APX was measured using the method described by Kim et al (2015). The reaction mixture comprised of 50 mM HEPES, 0.1 mM EDTA, 1 mM H 2 O 2 , and 0.6 mM ascorbic acid.…”

Section: Assay Of Antioxidant Enzyme Activitymentioning

confidence: 99%

Effect of drought acclimation on oxidative stress and transcript expression in wheat (Triticum aestivum L.)

Amoah

Yoon

et al. 2019

Journal of Plant Interactions

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“…In Pungsannamul, the MDA content increased significantly in response to increasing proton beam dose and the increase at a dose of 316 Gy was 46% compared to the control. Lipid peroxidation is a sign of oxidative damage, and the level of lipid peroxidation can be determined by measuring the MDA content, which is an end-product of lipid peroxidation, and has been used as indicator of plant membrane stress (Kim et al 2015;Lee et al 2015b). Lee et al (2015b) reported that MDA contents increased in response to increasing gamma-ray and proton beam doses, and that Cymbidium irradiated with proton beams at 100 MeV showed higher MDA contents than Cymbidium irradiated with gamma-rays.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Responses of Soybean (Glycine max L. Merr.) to Proton Beam Irradiation

Im¹,

Ko²,

Kim³

et al. 2017

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The present study evaluated the biochemical effects of proton beam irradiation in soybean. Seeds of two Korean elite cultivars (Kwangan and Pungsannamul) were irradiated by a 57-MeV proton beam in the range of 50-400 Gy. We measured the contents of malondialdehyde (MDA), antioxidant enzymes, and chlorophyll. MDA contents in proton beam-irradiated plants were higher than those in control plants. The activity of antioxidant enzymes differed between the two cultivars. In Kwangan, ascorbate peroxidase (APX) activity increased by 29% relative to the control at 55 Gy and decreased by 58% at 308 Gy. Superoxide dismutase (SOD) and peroxidase (POD) activities decreased by 47 and 25% relative to the control at 55 Gy and recovered to 87 and 56% of that at 55 Gy with 172 Gy and 117 Gy, respectively. In Pungsannamul, APX and SOD decreased by 32 and 35% relative to the control at 62 Gy, with the highest value observed at 243 Gy. In terms of the chlorophyll content, the two varieties responded similarly to proton beam irradiation, whereas in Kwangan, no significant reduction was observed above 100 Gy when compared with the control. Proton beam irradiation affected chlorophyll b more than chlorophyll a. These results show that the activity of antioxidant enzymes decreased in response to irradiation with approximately 50 Gy proton beams, then increased gradually with increasing doses, followed by a moderate decrease at higher doses. According to correlation with MDA contents and antioxidant enzyme activities, defense system of Pungsannamul was less activated by proton-beam irradiation than that of Kwangan.

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The effects of chronic radiation of gamma ray on protein expression and oxidative stress inBrachypodium distachyon

Abstract: This study provides some basic information regarding responses to gamma-irradiation, and provides valuable physiological and biological data on the effects of different gamma-irradiation dosages on Triticeae species.

Cited by 25 publications

References 81 publications

Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation

Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation

Effect of drought acclimation on oxidative stress and transcript expression in wheat (Triticum aestivum L.)

Biochemical Responses of Soybean (Glycine max L. Merr.) to Proton Beam Irradiation

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