The effects of certain nutritional conditions on the formation of purines and of ribonucleic acid in baker's yeast

Schmidt, Gerhard; Seraidarian, Krikor; Greenbaum, Lowell M.; Hickey, Mary D.; Thannhauser, S. J.

doi:10.1016/0006-3002(56)90272-4

Cited by 63 publications

(13 citation statements)

References 18 publications

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“…Although S-adenosylmethionine has multiple function as a biochemical intermediate and group donor (Cantoni, 1952;Schlenk et al, 1958), there is no explanation for the fact that yeasts, in comparison with other cells, in the presence of methionine can produce and accumulate such unusual amounts of S-adenosylmethionine. The increase in adenine concentration under the influence of methionine has been emphasized by Schmidt et al (1956) who noted a concomitant increase in the 03 -*-S-ADENOSYL- Control, and (3) cells containing S-adenosylmethionine, 24-hr culture at 30 C; (4) control, and (5) cells containing S-adenosylmethionine, 48-hr culture at 30 C; (6) control and (7) cells containing S-adenosylmethionine, 48-hr culture at 25 C.…”

Section: Discussionmentioning

confidence: 99%

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

Svihla

Schlenk

1959

J Bacteriol

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Supplementation of the culture medium of Candida utilis (Torulopsis utilis) and of Saccharomyces cerevisiae with L-methionine results in greatly increased formation and accumulation of S-adenosylmethionine (Schlenk and DePalma, 1957a), a sulfonium compound whose structure comprises adenosine and methionine in the following way: Adenine-ribose-S-CH2 CH2 CH(NH2) C OOH &H3 Under suitable conditions, a concentration of 40 to 60 Amoles per gram of yeast cells on a dry weight basis may be obtained. Because the molecular weight of the sulfonium ion is 399, this corresponds to a concentration of 1.6 to 2.4 per cent. S-Adenosylmethionine has the typical absorption spectrum of an adenosine compound with Em 15,400 at 260 m,u. Its concentration remains essentially constant after growth and metabolism have ceased and the quantity of nucleic acids has declined. It appeared possible, therefore, to determine the location of S-adenosylmethionine within the cells by ultraviolet microscopy. As a preliminary to the optical studies and for correlation, the earlier chemical studies had to be amplified. The present report is a detailed account of our observations which have been summarized earlier (Svihla and Schlenk, 1958). MATERIALS AND METHODS Candida utilis (Torulopsis utilis) strain ATCC 9950 was used in the present experiments. The composition of the growth medium, culture conditions, and harvesting procedure have been described earlier (Schlenk and DePalma, 1957b). L-Methionine in a concentration of 4 to 6 ,umoles per ml, or a combination of S-methyl-L-methionine and L-homocysteine (4 ,umoles of each) was used as organic sulfur supplement. For

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Section: Discussionmentioning

confidence: 99%

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

Svihla

Schlenk

1959

J Bacteriol

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“…Thus far, however, no direct evidence has been presented showing that the ATP pool is depleted. It has been suggested that, at low methionine concentrations, yeast preferentially use adenine groups for nucleic acid formation rather than for the production of S-AM (12). In this light, it seemed unreasonable to assume 542 that yeast would use all of their ATP pool to make S-AM, when normally a system of priorities exists which controls efficient use of adenine.…”

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confidence: 99%

Response of the Intracellular Adenosine Triphosphate Pool of Saccharomyces cerevisiae to Growth Inhibition Induced by Excess l -Methionine

Bailey

Parks

1972

J Bacteriol

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Yeast cells accumulate S-adenosyl-L-methionine (S-AM) when cultivated in the presence of L-methionine. Cell growth is inhibited by the addition of high concentrations of L-methionine. A number of investigators have attributed this to the depletion of adenosine triphosphate (ATP) as a consequence of the utilization of that mucleotide for S-AM formation. The cellular ATP pool of Saccharomyces cerevisiae was measured during growth inhibition caused by addition of excess L-methionine. Polyethylenimine thin-layer chromatography and subsequent autoradiography were used to quantitate the extracted ATP. Addition of L-methionine to a level of 5 mg/ml in a culture during exponential growth caused an increase in the doubling time of 40 to 50%. During this period, the cellular ATP level continued increasing normally and, as the cells entered stationary growth, receded to a level characteristic of an uninhibited stationary culture growth. After the addition of methionine, there was never an observed depletion of the ATP pool other than the normal fluctuation which occurs in an uninhibited culture. We have concluded that growth inhibition by excessive methionine does not result from limiting availability of ATP.

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“…However, the enzyme activity intensifies during the cultivation of the commercial yeast stage when the aeration increases and the P content decreases and during this P starvation condition, the acid phosphatase increases sharply, becoming 8-fold in 8 h as compared to the seed yeast (Suomalainen et al, 1960 ). An analogous behavior in phosphate-starved baker's yeast has been reported by other researchers (Schmidt et al, 1956 ). Similarly, Rautanin and Kaerkkaeinen ( 1951 ) discovered strong increase in acid phosphatase during incubation of Torulopsis utilis in a phosphate-deficient medium.…”

Section: Productionmentioning

confidence: 58%