2023
DOI: 10.3390/nu15153484
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The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line

Abstract: Adhesion is one of the main factors responsible for the probiotic properties of bacteria in the human gut. Membrane proteins affected by cellular damage are one of the key aspects determining adhesion. Fluid-bed-dried preparations containing probiotic bacteria were analyzed in terms of their stability (temperature of glass transition) and shelf life in different conditions (modified atmosphere, refrigeration). Imaging flow cytometry was utilized to determine four subpopulations of cells based on their physiolo… Show more

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Cited by 5 publications
(2 citation statements)
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References 32 publications
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“…However, probiotics must survive the technological processes necessary for their commercialization. Exposure to high temperatures or drying processes could reduce the ability of probiotics to adhere to intestinal cells [ 61 ].…”
Section: Resultsmentioning
confidence: 99%
“…However, probiotics must survive the technological processes necessary for their commercialization. Exposure to high temperatures or drying processes could reduce the ability of probiotics to adhere to intestinal cells [ 61 ].…”
Section: Resultsmentioning
confidence: 99%
“…The adhesion assay was performed according to the methodology described in our previous work [28]. The Caco-2 cells were prepared by first washing the monolayers twice with PBS to remove any loosely attached cells.…”
Section: Adhesion Assaymentioning
confidence: 99%