The effects of Caulerpa microphysa enzyme-digested extracts on ACE-inhibitory activity and in vitro anti-tumour properties

Lin, Hui-Chiu; Chou, Su‐Tze; Chuang, Mei-Yu; Liao, Tzu-Yen; Tsai, Wann-Sheng; Chiu, Tsai-Hsin

doi:10.1016/j.foodchem.2012.04.105

Cited by 23 publications

(7 citation statements)

References 27 publications

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“…The growth of both cell lines were significantly affected ( p < 0.05) when incubated with the digested extract at concentrations of 25 µg/mL and above. A significant increase in DNA damage was also recorded at concentrations of 100 µg/mL and above in comparison with the control cells [ 85 ].…”

Section: Anticancer Activity Of Seaweed Componentsmentioning

confidence: 99%

“…A cold water protein extract derived from Porphyra columbina (Rhodophyta) has been shown to have antihypertensive properties (>35% of ACE inhibition) [ 105 ]. C. microphysa pepsin digested extracts were determined to have greater ACE inhibitory activity than Flavourzyme or Alcalase [ 85 ]. Using enzyme hydrolysis, highly functional antihypertensive peptides have been produced from Porphyra yezoensis (Rhodophyta) Peptides produced under optimal conditions (1.5% substrate, 5% alcalase, pH 9.0, temperature of 50 °C and hydrolysis time of 60 minutes) had high antihypertensive activity (55% of ACE inhibition and a low IC 50 value of 1.6 g/L [ 106 ].…”

Section: Potential Of Seaweed Components To Alleviate Cardiovasculmentioning

confidence: 99%

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Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases

et al. 2016

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Seaweeds are a large and diverse group of marine organisms that are commonly found in the maritime regions of the world. They are an excellent source of biologically active secondary metabolites and have been shown to exhibit a wide range of therapeutic properties, including anti-cancer, anti-oxidant, anti-inflammatory and anti-diabetic activities. Several Asian cultures have a strong tradition of using different varieties of seaweed extensively in cooking as well as in herbal medicines preparations. As such, seaweeds have been used to treat a wide variety of health conditions such as cancer, digestive problems, and renal disorders. Today, increasing numbers of people are adopting a “westernised lifestyle” characterised by low levels of physical exercise and excessive calorific and saturated fat intake. This has led to an increase in numbers of chronic Non-communicable diseases (NCDs) such as cancer, cardiovascular disease, and diabetes mellitus, being reported. Recently, NCDs have replaced communicable infectious diseases as the number one cause of human mortality. Current medical treatments for NCDs rely mainly on drugs that have been obtained from the terrestrial regions of the world, with the oceans and seas remaining largely an untapped reservoir for exploration. This review focuses on the potential of using seaweed derived bioactives including polysaccharides, antioxidants and fatty acids, amongst others, to treat chronic NCDs such as cancer, cardiovascular disease and diabetes mellitus.

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Section: Anticancer Activity Of Seaweed Componentsmentioning

confidence: 99%

Section: Potential Of Seaweed Components To Alleviate Cardiovasculmentioning

confidence: 99%

Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases

et al. 2016

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“…turbinata exhibited the lowest (0.3822). Another study revealed that C. microphysa had potential ACE inhibitory activity as a result of pepsin cleavage [40]. However, the number of bioactive peptides in the BIOPEP database has increased, and so the frequency values might have been altered.…”

Section: Resultsmentioning

confidence: 99%

In Silico Analysis of Bioactive Peptides in Invasive Sea Grass Halophila stipulacea

Kandemır-Cavas

Pérez‐Sánchez

Mert-Ozupek

et al. 2019

Cells

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Halophila stipulacea is a well-known invasive marine sea grass in the Mediterranean Sea. Having been introduced into the Mediterranean Sea via the Suez Channel, it is considered a Lessepsian migrant. Although, unlike other invasive marine seaweeds, it has not demonstrated serious negative impacts on indigenous species, it does have remarkable invasive properties. The present in-silico study reveals the biotechnological features of H. stipulacea by showing bioactive peptides from its rubisc/o protein. These are features such as antioxidant and hypolipideamic activities, dipeptidyl peptidase-IV and angiotensin converting enzyme inhibitions. The reported data open up new applications for such bioactive peptides in the field of pharmacy, medicine and also the food industry.

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“…Chlorella sorokiniana can induce cytotoxicity in Hep G2 cells and inhibit the invasive capacity of malignant cells (Kim et al 2012). In a previous study, we reported that CME samples possessed high angiotensin-converting enzyme inhibitory activities and had a cytotoxic effect on WEHI-3 and HL-60 cells (Lin et al 2012). In this study, we examined the possible apoptotic mechanism of CME in WEHI-3 cells.…”

Section: Introductionmentioning

confidence: 91%

“…In a previous study, we reported that CME samples possessed high angiotensin‐converting enzyme inhibitory activities and had a cytotoxic effect on WEHI‐3 and HL‐60 cells (Lin et al . ). In this study, we examined the possible apoptotic mechanism of CME in WEHI‐3 cells.…”

Section: Introductionmentioning

confidence: 97%

Treatment with C aulerpa Microphysa Pepsin-Digested Extract Induces Apoptosis in Murine Leukemia WEHI-3 Cells

Chou

Lin

Chuang

et al. 2014

Journal of Food Biochemistry

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In this study, we examined the possible apoptotic mechanism of pepsin‐digested extract of Caulerpa microphysa (CME) in myelomonocytic leukemia (WEHI‐3) cells. Flow cytometry demonstrated that CME induced cell cycle arrest in the G0/G1 phase and stimulated reactive oxygen species (ROS) production and calcium release but caused a loss of the mitochondrial membrane potential (MMP). The results indicated that the protein levels of cyclin D, cyclin E, CDK6, CDK2 and Bcl‐2 decreased and those of p21, p27, p53, Bax, Bid, GRP78, GADD153, apoptosis‐inducing factor (AIF), caspase‐3 and caspase‐9 increased in WEHI‐3 cells after CME treatment. In conclusion, CME induced G0/G1 phase arrest, decreased MMP and increased Ca2+ release and ROS production in the WEHI‐3 cells. The results suggest that CME may have potential as an anticancer agent. Practical Applications Caulerpa microphysa (CME) has multiple functions and has been applied as natural seaweed extracts in the food and pharmaceutical industries. This study revealed for the first time that CME treatments inhibited the expression of the anti‐apoptotic protein and promoted the expression of pro‐apoptotic proteins. The results suggest that CME could be functional in food and pharmaceutical industries and could be an alternative anticancer medicine in the future.

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The effects of Caulerpa microphysa enzyme-digested extracts on ACE-inhibitory activity and in vitro anti-tumour properties

Cited by 23 publications

References 27 publications

Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases

Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases

In Silico Analysis of Bioactive Peptides in Invasive Sea Grass Halophila stipulacea

Treatment with C aulerpa Microphysa Pepsin-Digested Extract Induces Apoptosis in Murine Leukemia WEHI-3 Cells

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