The effects of burial on drug detection in skeletal tissues

Desrosiers, Nathalie A.; Watterson, James H.

doi:10.1002/dta.144

Cited by 6 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This fatty texture favors diffusion of the more lipophilic J o u r n a l P r e -p r o o f substances from the blood to the bone marrow [12] . Also for bone marrow, most studies that have been undertaken, use an animal model to investigate the influence of dosing and other parameters [6,[13][14][15] . Different categories of drugs are detected in bone marrow.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Skeletal tissue, a viable option in forensic toxicology? A view into post mortem cases

Vandenbosch

Rooseleers

Bogaert

et al. 2020

Forensic Science International

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Skeletal tissue, a viable option in forensic toxicology? A view into post mortem cases

Vandenbosch

Rooseleers

Bogaert

et al. 2020

Forensic Science International

View full text Add to dashboard Cite

show abstract

Comparison of Relative Distribution of Ketamine and Norketamine in Decomposed Skeletal Tissues Following Single and Repeated Exposures

Watterson

Donohue

Betit

2012

Journal of Analytical Toxicology

View full text Add to dashboard Cite

Bone was analyzed for ketamine and norketamine to examine whether different patterns of drug exposure could be discriminated. Rats received (intraperitoneally) one 75 mg/kg dose (Acute-1 and Acute-2 groups), three 25-mg/kg doses 1 hour apart (Repeated group), or nine single daily ketamine doses of 75 mg/kg followed by a 24-h washout period (Chronic group). Following euthanasia, all animals decomposed to skeleton outdoors. Ground samples of recovered bone underwent methanolic extraction and analysis by gas chromatography-mass spectrometry after solid-phase extraction. Drug levels (mass normalized response ratios) were compared across bone types and exposure pattern. Bone type significantly influenced drug level for the Acute-1 and Repeated dose groups, and the drug/metabolite level ratio (DMLR) for the Acute-1 group. Mean ketamine and norketamine level and DMLR varied by up to 8-fold, 7-fold and 3-fold, respectively, in the Acute-1 group, and by up to 24-fold, 5-fold and 10-fold, respectively, in the Repeated group. Drug level and DMLR differed significantly between the Acute-1 and Repeated groups for most bone types. In the Chronic group, only 1/16 and 4/16 samples were positive for ketamine and norketamine, respectively. All Acute-2 samples were positive for ketamine and norketamine. The Acute-2 and Chronic groups differed significantly in ketamine and norketamine levels, and DMLR.

show abstract

Analysis of Dextromethorphan and Dextrorphan in Skeletal Remains Following Decomposition in Different Microclimate Conditions

Unger

Watterson

2016

J Anal Toxicol

View full text Add to dashboard Cite

The effects of decomposition microclimate on the distribution of dextromethorphan (DXM) and dextrorphan (DXT) in skeletonized remains of rats acutely exposed to DXM were examined. Animals (n = 10) received DXM (75 mg/kg, i.p.), were euthanized 30 min post-dose and immediately allowed to decompose at either Site A (shaded forest microenvironment on a grass-covered soil substrate) or Site B (rocky substrate exposed to direct sunlight, 600 m from Site A). Ambient temperature and relative humidity were automatically recorded 3 cm above rats at each site. Skeletal elements (vertebral columns, ribs, pelvic girdles, femora, tibiae, humeri and scapulae) were harvested, and analyzed using microwave assisted extraction, microplate solid phase extraction, and GC/MS. Drug levels, expressed as mass-normalized response ratios, and the ratios of DXT and DXM levels were compared across bones and between microclimate sites. No significant differences in DXT levels or metabolite/parent ratios were observed between sites or across bones. Only femoral DXM levels differed significantly between microclimate sites. For pooled data, microclimate was not observed to significantly affect analyte levels, nor the ratio of levels of DXT and DXM. These data suggest that microclimate conditions do not influence DXM and metabolite distribution in skeletal remains.

show abstract

The effects of burial on drug detection in skeletal tissues

Cited by 6 publications

References 23 publications

Skeletal tissue, a viable option in forensic toxicology? A view into post mortem cases

Skeletal tissue, a viable option in forensic toxicology? A view into post mortem cases

Comparison of Relative Distribution of Ketamine and Norketamine in Decomposed Skeletal Tissues Following Single and Repeated Exposures

Analysis of Dextromethorphan and Dextrorphan in Skeletal Remains Following Decomposition in Different Microclimate Conditions

Contact Info

Product

Resources

About