The Effects of Branched Chain Amino Acid Supplement on Kidney Tissue of Exercising Rats

Abstract

One of the supplements used in with exercise programs is branched chain amino acids (BCAAs) which preferred because of their effect on novation of muscle protein synthesis. However, BCAAs increase their amount in the blood in a short time due to their properties. In this case, the result can increase the workload of the kidneys. Based on the information, this study investigated the effects of resistance exercise and BCAA supplements on kidney tissue. A total of 24 Wistar Albino male rats were divided equally into 4 groups: Control, BCAA, Exercise and Exercise+BCAA. In six-week study, resistance swimming exercise was applied to exercise groups. BCAA groups were given BCAA supplements at doses of 2.5 mg/kg before exercise. End of the study, histological, immunochemical, and RT-PCR analyses were performed. As a result of the findings obtained, it was found that use of BCAA supplements together with exercise caused tubular necrosis (p=0.002). A significant increase was found in caspase 3 IHC staining findings in BCAA and Exercise+BCAA groups compared to Control group (p=0.011; p=0.02). Also, KIM-1 expression levels were higher in Exercise group compared to all other groups (p=0.004; p=0.003; p=0.008). As a result, BCAA consumption in combination with resistant exercise caused damage to kidney tissue.