The effects of BMPs on early chick embryos suggest a conserved signaling mechanism for epithelial and neural induction among vertebrates

Gallego-Dı́az, Victoria; Schoenwolf, Gary C.; Álvarez, Ignacio S.

doi:10.1016/s0361-9230(01)00716-x

Cited by 5 publications

(5 citation statements)

References 13 publications

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“…Daoud et al found that expression of ectopic GATA4 or GATA5 can mimic the effects of BMP administration on paraxial mesoderm and similarly block the induction of hedgehog-responsive genes [54]. Gallego-Dı´az reported that the overexpression of several members of the BMP family of growth factors changes the normal development of the epithelial and neural tissues in early chick embryos [55]. Increased BMP signaling from the border between the areas of pellucida and opaca increases the epithelial territory as denoted by the expression of Dlx5.…”

Section: Discussionmentioning

confidence: 99%

One-stop assembly of adherent 3D retinal organoids from hiPSCs based on 3D-printed derived PDMS microwell platform

et al. 2023

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The three-dimensional (3D) retinal organoids (ROs) derived from human induced pluripotent stem cells (hiPSCs), mimicking the growth and development of the human retina, is a promising model for investigating inherited retinal diseases (IRD) in vitro. However, the efficient generation of homogenous ROs remains a challenge. Here we introduce a novel Polydimethylsiloxane (PDMS) microwell platform containing 62 V-bottom micro-cavities for the ROs differentiation from hiPSCs. The uniform adherent 3D ROs could spontaneously form using neural retina (NR) induction Our results showed that the complex of NR (expressing VSX2), ciliary margin (CM) (expressing RDH10), and retinal pigment epithelium (RPE) (expressing ZO-1, MITF, and RPE65) developed in the PDMS microwell after the differentiation. It is important to note that ROs in PDMS microwell platforms not only enable one-stop assembly but also maintain homogeneity and mature differentiation over a period of more than 25 weeks without the use of BMP4 and Matrigel. Retinal ganglion cells (expressing BRN3a), amacrine cells (expressing AP2a), horizontal cells (expressing PROX1 and AP2α), photoreceptor cells for cone (expressing S-opsin L/M-opsin) and rod (expressing Rod opsin), bipolar cells (expressing VSX2 and PKCα), and Müller glial cells (expressing GS and Sox9) gradually emerged. Furthermore, we replaced fetal bovine serum (FBS) with human platelet lysate (HPL) and established a xeno-free culture workflow that facilitates clinical application. Thus, our PDMS microwell platform for one-stop assembly and long-term culture of ROs using a xeno-free workflow is favorable for retinal disease modeling, drug screening, and manufacturing ROs for clinical translation.

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Section: Discussionmentioning

confidence: 99%

One-stop assembly of adherent 3D retinal organoids from hiPSCs based on 3D-printed derived PDMS microwell platform

et al. 2023

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“…Blastulationstage embryos lost their pluripotency state during the diapause extension phase; however, activation of the BMP4 pathway in these embryos restored the lost pluripotent state, thus highlighting the plasticity of blastodermal cells to regain pluripotency. The induced pluripotency in vivo in blastulating embryos in diapause represents a molecular event that is rarely attainable in vivo in humans, but is widely studied in vitro in mammalian cells (Gallego-Díaz, Schoenwolf, and Alvarez 2002;Tapia et al 2012) this phenomenon is key to development and regeneration in non-mammalian vertebrates, including teleost fish, urodele amphibians, and lizards (reviewed in Zahumenska et al 2020).…”

Section: Roles Of the Bmp4 Pathway In Maintaining Embryos' Ability To Successfully Resume Developmentmentioning

confidence: 99%

How do avian embryos resume development following diapause? A new role for TGF-β in regulating pluripotency-related genes

Pokhrel

Genin

Sela-Donenfeld

et al. 2021

Preprint

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Avian embryos can halt their development for long periods at low temperature in a process called diapause and successfully resume development when reincubated at maternal body temperature. Successful resumption of development depends on different factors, including temperature. We have recently shown that embryos that enter diapause at 18 °C present a significant reduction in their ability to develop normally when put back into incubation, compared to embryos entering diapause at 12 °C. However, the mechanisms underlying these differences are unknown. To address this question, transcriptome analysis was performed to compare the effect of diapause temperature on gene expression, and to identify pathways involved in the process. Genetic comparison and pathway-enrichment analysis revealed that TGF-β and pluripotency-related pathways are differentially regulated at the two temperatures, with higher expression at 12 °C compared to 18 °C. Investigating the involvement of the TGF-β pathway revealed an essential role for BMP4 in regulating the expression of the transcription factors Nanog and Id2, which are known to regulate pluripotency and self-renewal in embryonic stem cells. BMP4 gain- and loss-of-function experiments in embryos in diapause at the different temperatures revealed the main role of BMP4 in enabling resumption of normal development following diapause. Collectively, these findings identify molecular regulators that facilitate embryos’ ability to undergo diapause at different temperatures and resume a normal developmental program.

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“…It has been shown that BMP2 and BMP4 are highly expressed in the germinal crescent region of the chicken embryo (Gallego-Díaz et al, 2002;Puelles et al, 2005). Thus, early germ cell development in both mice and chickens occurs in a BMP-positive extracellular environment.…”

Section: Germ Cell-specific Genes and Determinants Identified In Chickenmentioning

confidence: 99%

“…BMP2 increases cell reactivity to SSEA1. (Gallego-Díaz et al, 2002;Puelles et al, 2005;Kim et al, 2006).…”

Section: Cnanogmentioning

confidence: 99%

Primordial Germ Cell Technologies for Avian Germplasm Cryopreservation and Investigating Germ Cell Development

Glover

McGrew

2012

J. Poult. Sci.

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The germ cell lineage is unique as it is the cell lineage that ensures the continuation and the variation of genetic information from one generation to the next. Primordial germ cells are the stem cell precursors of this lineage differentiating into mature spermatozoa and oocytes in the adult. The recent development of cell culture conditions for the extended propagation of chicken primordial germ cells isolated from embryonic blood heralds the emergence of a technology that will have a considerable impact on the management of avian resources, efforts in avian species preservation and transgenic technologies. This review will address what is known of the formation of avian germ cells, the current state of the field, and discuss the prospects that this technology offers for both developmental biology and the preservation of avian germplasm.

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The effects of BMPs on early chick embryos suggest a conserved signaling mechanism for epithelial and neural induction among vertebrates

Cited by 5 publications

References 13 publications

One-stop assembly of adherent 3D retinal organoids from hiPSCs based on 3D-printed derived PDMS microwell platform

One-stop assembly of adherent 3D retinal organoids from hiPSCs based on 3D-printed derived PDMS microwell platform

How do avian embryos resume development following diapause? A new role for TGF-β in regulating pluripotency-related genes

Primordial Germ Cell Technologies for Avian Germplasm Cryopreservation and Investigating Germ Cell Development

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