The Effects of Biosynthetic Insulin-Like Growth Factor-1 Supplementation on Somatic Growth, Maturation, and Erythropoiesis on the Neonatal Rat

Philipps, Anthony F; Persson, Bengt; Hall, Kerstin; Lake, Mats; Skottner, A.; Sanengen, Truls; Sara, Vicki R.

doi:10.1203/00006450-198803000-00014

Cited by 100 publications

(40 citation statements)

References 37 publications

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“…The changes observed in IGF-I in this study are consistent with previous data evaluating changes in IGF-I in the growthretarded fetus and in early postnatal life (8)(9)(10)22). Fetal rat pups rendered growth-retarded by uterine artery ligation were found to have depressed serum and tissue levels of IGF-I when compared with controls (9).…”

Section: Resultssupporting

confidence: 81%

Insulin-Like Growth Factor I in Substrate-Deprived, Growth-Retarded Fetal Rats

1991

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ABSTRACF. Plasma, amniotic fluid, and tissue concentra-Control animals were allowed free access to both food and water. tions of IGF-I were examined in nutritionally deprived, Sampling was performed on d 2 1 in all animals, after intraperigrowth-retarded fetal rats to determine whether IGF-I toneal pentobarbital (45 mg/kg) administration. Midline lapaconcentration serves as a marker for nutritional status. rotomy incision was used to expose the uterine horns. Fetuses in Growth retardation was induced by 72 h of maternal fast-each horn were counted, and the two inferior fetuses of each ing. Twenty-three control and 17 growth-retarded fetuses horn were sampled to match the control and test group with were individually analyzed and compared. Plasma IGF-I regard to intrauterine location. A maximum of four fetuses per concentrations were significantly lower in test compared dam were analyzed to insure matching of uterine location bewith control animals (test 56.8 f 14.9, control 87.4 f 17.5 tween groups and to minimize any metabolic impact of the ng/mL, p < 0.01). Amniotic fluid IGF-I concentrations surgical procedure. Only those fetuses in whom results from all were not different (test 14.0 f 8.7, control 12.2 f 2.6 ng/ specimens were available were included in the analyses. The mL). IGF-I concentrations obtained from both placental sampling sequence was initiated by the removal of amniotic fluid and hepatic tissues were lower in test compared with with a 19-or 20-gauge 1-mL syringe. The fetus was then removed control animals [placenta: test 293 f 25 versus control 655 from its location in the uterine horn, and an axillary cutdown f 114 ng/g (p < 0.001); hepatic: test 173 k 38 versus was performed (15). Fetal blood was collected by heparinized control 230 f 51 ng/g ( p < 0.01)]. Reductions in fetal, capillary tube, placed on ice, and centrifuged for 15 min at 12 placental, and hepatic weights in test animals were more 000 X g. The fetus was separated from the placenta, and the closely related to changes in placental IGF-I concentration fetus, placenta, and then fetal liver were weighed. The placenta than to either plasma or hepatic IGF-I concentrations. We and liver were quickfrozen in liquid nitrogen. All samples were conclude that fetal plasma IGF-I is a valuable marker for stored at -80°C until analysis. After fetal sampling, a maternal intrauterine substrate deprivation and that the growth-venous plasma sample was obtained from the inferior vena cava retarded rat fetus is accurately identified and specifically just below the renal vein. Euthanasia was performed by means characterized by a low placental concentration of extract-of exsanguination after all samples had been obtained. Individual Analyses. IGF-I concentrations in maternal and fetal plasma and amniotic fluid were determined by RIA [National Institute Circulating concentrations of IGF-I are influenced by both of Diabetes and Digestive and Kidney Diseases somatomedin-C growth hormone and nutritional status (1-10). Although the antiserum (polyclonal) cod...

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Section: Resultssupporting

confidence: 81%

Insulin-Like Growth Factor I in Substrate-Deprived, Growth-Retarded Fetal Rats

1991

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“…Absorption of biologic ally active IGFs to organs distant to the gut could not be documented, and whether or not absorption and transport had already occurred before the conclusion of the experiments is unknown. However, the finding of large molecul ar weight fragments of IGF-I in neonatal rat skin is intriguing and warrants future studies, particularly in view of a previous finding of enhanced skin epithelial maturation in rat pups injected with a similar recombinant IGF-I preparation (29).…”

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confidence: 99%

Fate of Insulin-Like Growth Factors I and II Administered Orogastrically to Suckling Rats

et al. 1995

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“…A Consensus Development Conference on Treatment of Destructive Behaviors in Persons with Developmental Disabilities will be held in Masur Auditorium in the Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD, September 1 [1][2][3][4][5][6][7][8][9][10][11][12][13]1989. This open forum will focus on the various treatments and approaches used to modify or eliminate destructive behaviors in persons with developmental disabilities.…”

Section: Announcementmentioning

confidence: 99%

“…After weaning, the predominant somatomedin species is an IGF-I similar to human IGF-I (3,4,7). Unlike the adult, GH does not seem to be of importance in IGF regulation in the preweanling rat (8).…”

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The Effects of Altered Nutritional Status upon Insulin-Like Growth Factors and their Binding Proteins in Neonatal Rats

et al. 1989

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ABSTRACT. To investigate the role of nutrition in the regulation of IGFs during the perinatal period, 10-d-old rats were infused intravenously with various concentrations of nutrients for 24 h. Breast-fed litter mates served as controls. The effect of caloric intake on concentrations of IGF-I and IGF-I1 as well as IGF-binding proteins in serum, liver, and brain of neonatal rats was studied. A total of 45 rats from 10 litters was infused with solutions ranging from a caloric intake of 0 (saline) to 75% (glucose, amino acids, and lipids) of the estimated intake of control rats. In serum, both IGF-I and -11 concentrations fell markedly in response to fasting. Serum IGF-I1 levels were linearly related to caloric intake in the pooled data from all groups. Concentrations of IGF-11, but not IGF-I, in liver and brain were depressed by caloric restriction. In contrast to the fall in IGF concentrations, activity of IGF-associated binding proteins rose in serum and in liver cytosol 2-to 4-fold in response to decreased nutrient intake. In serum, but not liver, the rise in binding protein activity was inversely related to caloric intake. In liver cytosol, but not serum, the rise in binding protein activity was inversely related to total serum amino acid concentration. Thus, IGF concentrations in preweanling rats change in response to alterations of nutrient intake. The fasting induced decrements in IGF levels, as well as the elevations in IGF-associated binding protein activity, may serve as a protective mechanism to depress growth in times of caloric restriction. (Pediatr Res 26: 128-134,1989) Abbreviations CON, control SAL, normal saline GLU, glucose GA, glucose and amino acids GAL, glucose, amino acids, and lipid RRA, radioreceptor assay HSA, human serum albumin GH, growth hormone

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The Effects of Biosynthetic Insulin-Like Growth Factor-1 Supplementation on Somatic Growth, Maturation, and Erythropoiesis on the Neonatal Rat

Cited by 100 publications

References 37 publications

Insulin-Like Growth Factor I in Substrate-Deprived, Growth-Retarded Fetal Rats

Insulin-Like Growth Factor I in Substrate-Deprived, Growth-Retarded Fetal Rats

Fate of Insulin-Like Growth Factors I and II Administered Orogastrically to Suckling Rats

The Effects of Altered Nutritional Status upon Insulin-Like Growth Factors and their Binding Proteins in Neonatal Rats

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