The effects of Biodentine/polycaprolactone three‐dimensional‐scaffold with odontogenesis properties on human dental pulp cells

Ho, Cheng-Yuan; Fang, Hsin Yuan; Wang, B.; Huang, Tsui‐Hsien; Shie, Ming‐You

doi:10.1111/iej.12799

Cited by 39 publications

(42 citation statements)

References 39 publications

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“…) and Biodentine (Ho et al . ) enhanced the differentiation of human dental pulp cells in favour of osteogenesis in in vitro models. Additional studies evaluating new materials may expand the utility of 3D printing to regenerative endodontics, guided tissue regeneration and wound healing.…”

Section: Discussionmentioning

confidence: 98%

“…After nearly two decades of 3D printing in dentistry, the first certified biocompatible resin, NextDent SG (3D Systems, Soesterberg, Netherlands) was introduced in 2016. 3D printed bioscaffolds containing Mineral Trioxide Aggregate (Chiu et al 2017) and Biodentine (Ho et al 2018) enhanced the differentiation of human dental pulp cells in favour of osteogenesis in in vitro models. Additional studies evaluating new materials may expand the utility of 3D printing to regenerative endodontics, guided tissue regeneration and wound healing.…”

Section: Discussionmentioning

confidence: 99%

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Endodontic applications of 3D printing

Anderson¹,

Wealleans²,

Ray³

2018

Int Endodontic J

139

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Anderson J, Wealleans J, Ray J. Endodontic applications of 3D printing. International Endodontic Journal.Computer-aided design (CAD) and computer-aided manufacturing (CAM) technologies can leverage cone beam computed tomography data for production of objects used in surgical and nonsurgical endodontics and in educational settings. The aim of this article was to review all current applications of 3D printing in endodontics and to speculate upon future directions for research and clinical use within the specialty. A literature search of PubMed, Ovid and Scopus was conducted using the following terms: stereolithography, 3D printing, computer aided rapid prototyping, surgical guide, guided endodontic surgery, guided endodontic access, additive manufacturing, rapid prototyping, autotransplantation rapid prototyping, CAD, CAM. Inclusion criteria were articles in the English language documenting endodontic applications of 3D printing. Fifty-one articles met inclusion criteria and were utilized. The endodontic literature on 3D printing is generally limited to case reports and pre-clinical studies. Documented solutions to endodontic challenges include: guided access with pulp canal obliteration, applications in autotransplantation, pre-surgical planning and educational modelling and accurate location of osteotomy perforation sites. Acquisition of technical expertise and equipment within endodontic practices present formidable obstacles to widespread deployment within the endodontic specialty. As knowledge advances, endodontic postgraduate programmes should consider implementing 3D printing into their curriculums. Future research directions should include clinical outcomes assessments of treatments employing 3D printed objects.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Endodontic applications of 3D printing

Anderson¹,

Wealleans²,

Ray³

2018

Int Endodontic J

139

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“…Highly porous poly-lactic acid scaffolds doped with bioactive calcium silicates and calcium phosphates have been recently designed [28,29] and demonstrated the ability to be colonized by oral derived mesenchymal stem cells and to stimulate their shift through osteogenic lineage [27,36]. Calcium silicates/poly-e-caprolactone 3D printed scaffolds designed for dental pulp tissues revascularization purposes showed good dental pulp stem cell proliferation and osteogenic differentiation [37].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, Si may be localized in young bone active calcification sites [39]. Si from CaSi (such as bioglasses, ackermanite, wollanstonite) is able to increase gene expression of VEGF and FGF, two important pro-angiogenic cytokines [20,37] and upregulate the downstream signalling of nitric oxide synthesis, genes and activity [19,40,41]. Several CaSi (ackermanite and wollanstonite) were able to trigger the secretion of angiogenic growth factors from fibroblasts, with the result of a higher number of blood vessel infiltration intro tissue-engineered scaffolds [41].…”

Section: Discussionmentioning

confidence: 99%

Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

et al. 2020

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Vascularization is a crucial factor when approaching any engineered tissue. Vascular wall-mesenchymal stem cells are an excellent in vitro model to study vascular remodeling due to their strong angiogenic attitude. This study aimed to demonstrate the angiogenic potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dihydrate (DCPD), namely PLA-10CaSi-10DCPD and PCL-10CaSi-10DCPD, designed for the regeneration of bone defects. Vascular wall-mesenchymal stem cells (VW-MSCs) derived from pig thoracic aorta were seeded on the scaffolds and the expression of angiogenic markers, i.e. CD90 (mesenchymal stem/stromal cell surface marker), pericyte genes α-SMA (alpha smooth muscle actin), PDGFR-β (platelet-derived growth factor receptor-β), and NG2 (neuron-glial antigen 2) was evaluated. Pure PLA and pure PCL scaffolds and cell culture plastic were used as controls (3D in vitro model vs. 2D in vitro model). The results clearly demonstrated that the vascular wall mesenchymal cells colonized the scaffolds and were metabolically active. Cells, grown in these 3D systems, showed the typical gene expression profile they have in control 2D culture, although with some main quantitative differences. DNA staining and immunofluorescence assay for alpha-tubulin confirmed a cellular presence on both scaffolds. However, VW-MSCs cultured on PLA-10CaSi-10DCPD showed an individual cells growth, whilst on PCL-10CaSi-10DCPD scaffolds VW-MSCs grew in spherical clusters. In conclusion, vascular wall mesenchymal stem cells demonstrated the ability to colonize PLA and PCL scaffolds doped with CaSi-DCPD for new vessels formation and a potential for tissue regeneration.

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“…MTA is a popular endodontic material, which is commonly used as a filling material for root endings, pulp capping material, and also for perforation repair [ 14 , 39 , 40 ]. Despite having many advantages, its long setting timings had severely limited its use for clinical applications [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Mineral Trioxide Aggregate Mixed with 5-Aminolevulinic Acid for the Photodynamic Antimicrobial Strategy in Hard Tissue Regeneration

Huang

et al. 2018

Materials

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Aminolevulinic acid (ALA) based photodynamic antimicrobial strategy can provide good antimicrobial effects and be used for medical applications. The aim of this study was to apply this strategy to Mineral Trioxide Aggregate (MTA), which is commonly used as a filling material for root endings and by doing so, to increase the bactericidal capability of MTA, as well as to investigate its characterization, cytocompatibility, and odontogenic differentiation potential. MTA is known to be a derivative of calcium silicate (CS). In this study, MTA specimens with or without ALA and light treatment were prepared. Diametral tensile strength values (DTS), setting durations, X-ray diffraction (XRD) spectra, apatite-mineralization, and antimicrobial abilities of the MTA, were also analyzed. Human dental pulp cells (hDPCs) can proliferate into the newly formed matrix and differentiate into odontoblasts to reinforce and strengthen the root. Levels of hDPCs proliferation and its odontogenic capabilities when cultured on MTA with ALA and light treatment, and the percentages of cells existing in the various cell cycle stages, were further evaluated in this study. The results indicated that MTA added ALA with light treatment had greater antibacterial ability and cytocompatibility, compared to MTA alone. A higher percentage S phase of the cells cultured on MTA added ALA with light treatment was observed. Furthermore, hDPCs cultured on MTA added ALA with light treatment had the highest expression levels of the odontoblastic differentiation markers. ALA has great antimicrobial efficiency and is a potential material for future medical applications. ALA-based photodynamic antibacterial strategy applied in the MTA has great antibacterial ability, cytocompatibility, and odontoblastic differentiation potential, and can facilitate the development of root canal treatment.

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The effects of Biodentine/polycaprolactone three‐dimensional‐scaffold with odontogenesis properties on human dental pulp cells

Cited by 39 publications

References 39 publications

Endodontic applications of 3D printing

Endodontic applications of 3D printing

Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

Mineral Trioxide Aggregate Mixed with 5-Aminolevulinic Acid for the Photodynamic Antimicrobial Strategy in Hard Tissue Regeneration

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