The Effects of Ba-wei-wan (Hachimijiogan) on Plasma Levels of High Density Lipoprotien-cholesterol and Lipoperoxide in Aged Individuals

Yoshida, Hiroshi; Kusukawa, R; Watanabe, Nobuhisa; Ohtsuki, Gen; Sakamoto, Tatsuhiro; Haranaka, Ruriko

doi:10.1142/s0192415x85000113

Cited by 3 publications

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“…Our study of transfection into CV1 cells clearly showed that Hachimi-jio-gan activated DR1 luciferase activity and this activation is PPARα-specific. Hachimi-jio-gan was reported to have favorable effects on lipid [11] and glucose metabolism [5,13]; however, this mechanism has not been resolved. Our study suggested one possibility that the effect of Hachimi-jio-gan was induced by PPARα activation.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Hachimijio-gan was reported to suppress hyperglycemia via insulin production and secretion from the pancreas [5]. In addition, Hachimi-jio-gan has been widely used for the treatment of many chronic diseases such as renal nephritis [6], vegetative ataxia [7], major depressive disorder [8,9], hyperprolactinemia [10], and hyperlipidemia [11]; however, the mechanisms of Hachimijio-gan remain unclear. The objective of this study was to investigate whether Hachimi-jio-gan has functions related to hormonal effects using transfection assays with thyroid hormone receptor, androgen receptor, estrogen receptor, estrogen receptor, RXR, PPARγ, and PPARα into several cell lines.…”

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confidence: 99%

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Herbal Medicine, Hachimi-jio-gan, and Its Component Cinnamomi Cortex Activate the Peroxisome Proliferator-activated Receptor Alpha in Renal Cells

et al. 2008

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Abstract. Hachimi-jio-gan is widely used to improve several disorders associated with diabetes, but its mechanism remains poorly understood. In an attempt to clarify the mechanism of Hachimi-jio-gan, we investigated the effects of this herbal medicine and its components in transfection studies of CV1 cells, especially nuclear receptor-mediated actions. One half (0.5) mg/ml of Hachimi-jio-gan activated peroxisome proliferator-activated receptor (PPARα), mediating the activation by 3.1-fold on DR1 response elements; however, it did not affect PPARγ, thyroid hormone receptor, androgen receptor, estrogen receptor or RXR. In addition, this activation was observed in a dose-dependent manner. Next, to determine which components of Hachimi-jio-gan activate PPARα-mediated transcription, 8 of its components (rehmanniae radix, orni fructus, dioscoreae rhizoma, alismatis rhizoma, hoelen, moutan cortex, cinnamomi cortex, aconiti) were tested. Only cinnamomi cortex (1.0 mg/ml) increased PPARα-mediated transcription by 4.1-fold, and this activation was specific for PPARα, and not for other nuclear receptors. Moreover, this PPARα-related activation by cinnamomi cortex is specifically observed in renal cells. Taken together, these findings indicate that Hachimi-jio-gan and cinnamomi cortex may have a pharmacological effect through the target site for PPARα. IN Japan and China, traditional herbal medicines (Kampo) have often been used for diabetes mellitus to treat symptoms such as fever, numbness, and edema. In particular, Hachimi-jio-gan is used clinically to improve diabetic nephropathy [1][2][3][4]. Recently, Hachimijio-gan was reported to suppress hyperglycemia via insulin production and secretion from the pancreas [5]. In addition, Hachimi-jio-gan has been widely used for the treatment of many chronic diseases such as renal nephritis [6], vegetative ataxia [7], major depressive disorder [8,9], hyperprolactinemia [10], and hyperlipidemia [11]; however, the mechanisms of Hachimijio-gan remain unclear. The objective of this study was to investigate whether Hachimi-jio-gan has functions related to hormonal effects using transfection assays with thyroid hormone receptor, androgen receptor, estrogen receptor, estrogen receptor, RXR, PPARγ, and PPARα into several cell lines. Moreover, Hachimi-jio-gan is composed of eight ingredients, rehmanniae radix, orni fructus, dioscoreae rhizoma, alismatis rhizoma, hoelen, moutan cortex, cinnamomi cortex and aconiti; therefore, we identified which components of Hachimi-jio-gan are responsible for pharmacological effects such as hormonal ligands for nuclear receptors.

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Section: Discussionmentioning

confidence: 99%