The effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen

Hernández‐Avilés, Camilo; Serafini, R.; Love, Charles C.; Teague, S.R.; LaCaze, K.A.; Lawhon, Sara D.; Wu, Jing; Blanchard, T.L.; Varner, D.D.

doi:10.1016/j.theriogenology.2018.08.022

Cited by 22 publications

(13 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, it was reported that the addition of clavulanic acid-associated ticarcillin (Timentin ® ) would be more effective for the bacterial control during equine semen preservation than the isolate use of INRA 96 ® diluent (Olivieri et al,2011). In addition, the use of the potassium penicillin-amikacin combination has also been evidenced for providing efficient antibacterial action and maintaining sperm parameters during equine semen storage (Varner et al, 1998;Hernández-Avilés et al, 2018;Hernández-Avilés et al, 2019).…”

Section: Use Of Antibacterial Substances In Semen Extendersmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

View full text Add to dashboard Cite

How to cite: Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology. AbstractThe use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Among the various mammalian species, there is a large variation regarding the antimicrobial types added to semen extenders as cephalosporins (ceftiofur, cefdinir, eg) and quinolones (ofloxacin, ciprofloxacin), alone or in combination with large action spectra substances as penicillin-streptomycin and gentamicin-tylosin-lincomycin-spectinomycin. To combat problems related to bacterial resistance to these drugs, the emergence of alternatives is increasingly evident. Among these alternatives, use of physical methods as centrifugation and filtration, as well as the use of antimicrobial peptides and other substances from different origins have been highlighted for presenting antimicrobial potential.

show abstract

Section: Use Of Antibacterial Substances In Semen Extendersmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

View full text Add to dashboard Cite

show abstract

“…aeruginosa inoculated into semen. However, these antimicrobials did not inhibit growth when higher numbers of the bacteria were inoculated [ 63 ]. The presence of biofilms, such as in some cases of chronic endometritis, may render antibiotics ineffective.…”

Section: Bacteria In Semenmentioning

confidence: 99%

Antimicrobial Resistance in Equine Reproduction

Malaluang

Wilén

Lindahl

et al. 2021

Animals

View full text Add to dashboard Cite

Bacteria develop resistance to antibiotics following low-level “background” exposure to antimicrobial agents as well as from exposure at therapeutic levels during treatment for bacterial infections. In this review, we look specifically at antimicrobial resistance (AMR) in the equine reproductive tract and its possible origin, focusing particularly on antibiotics in semen extenders used in preparing semen doses for artificial insemination. Our review of the literature indicated that AMR in the equine uterus and vagina were reported worldwide in the last 20 years, in locations as diverse as Europe, India, and the United States. Bacteria colonizing the mucosa of the reproductive tract are transferred to semen during collection; further contamination of the semen may occur during processing, despite strict attention to hygiene at critical control points. These bacteria compete with spermatozoa for nutrients in the semen extender, producing metabolic byproducts and toxins that have a detrimental effect on sperm quality. Potential pathogens such as Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa may occasionally cause fertility issues in inseminated mares. Antibiotics are added during semen processing, according to legislation, to impede the growth of these microorganisms but may have a detrimental effect on sperm quality, depending on the antimicrobial agent and concentration used. However, this addition of antibiotics is counter to current recommendations on the prudent use of antibiotics, which recommend that antibiotics should be used only for therapeutic purposes and after establishing bacterial sensitivity. There is some evidence of resistance among bacteria found in semen samples. Potential alternatives to the addition of antibiotics are considered, especially physical removal separation of spermatozoa from bacteria. Suggestions for further research with colloid centrifugation are provided.

show abstract

“…Semen plasma contains antimicrobial properties that can reduce bacterial growth. Proteins and peptides contained in semen plasma are active against antimicrobials, including E. coli and Pseudomonas spp [17]. However, it still needs to add antibacterial ingredients to minimize bacterial growth further.…”

Section: Total Bacteriamentioning

confidence: 99%

Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Sitepu

Udin

Jaswandi

et al. 2020

AJBGMB

View full text Add to dashboard Cite

Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing). Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P <0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4). Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.

show abstract

The effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen

Cited by 22 publications

References 27 publications

Current and alternative trends in antibacterial agents used in mammalian semen technology

Current and alternative trends in antibacterial agents used in mammalian semen technology

Antimicrobial Resistance in Equine Reproduction

Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

Contact Info

Product

Resources

About