The Effects of Amoxicillin, Cefazolin, and Gentamicin Antibiotics on the Antioxidant System in Mouse Heart Tissues

Abstract

Background: Free radicals lead to destruction in various organs of the organism. The improper use of antibiotics increases the formation of free radicals and causes oxidative stress. Objective: In this study, it was aimed to determine the effects of gentamicin, amoxicillin, and cefazolin antibiotics on the mouse heart. Methods: 20 male mice were divided into 4 groups (1st control, 2nd amoxicillin, 3rd cefazolin, and 4th gentamicin groups). The mice in the experimental groups were administered antibiotics intraperitoneally at a dose of 100 mg / kg for 6 days. The control group received normal saline in the same way. The gene expression levels and enzyme activities of SOD, CAT, GPx, GR, GST, and G6PD antioxidant enzymes were investigated. Results : GSH levels decreased in both the amoxicillin and cefazolin groups, while GR, CAT, and SOD enzyme activities increased. In the amoxicillin group, Gr, Gst, Cat, and Sod gene expression levels increased. Conclusion: As a result, it was concluded that amoxicillin and cefazolin caused oxidative stress in the heart, however, gentamicin did not cause any effects.

Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower

“…Regardless of the concentration, the extract could not prevent the reactivity of H2O2 and could not fix the damage caused by H2O2 (Figure 2, wells: 8, 9, 10). Although this result is quite thought-provoking, it is well known that the excessive amount of H2O2 in a cell is eliminated by the activities of glutathione peroxidase (GPx) and catalase (CAT) [6] however, these reactions may terminate in the herbarium material. This information may explain the reason why the extract does not show DNA protective activity and suggests data that, independent of enzymatic catalysis, H2O2 cannot be effectively eliminated in a cell.…”

“…As a summary of the DNA protective activity result that supports the antioxidant activity result, there are some phenolics in the herbarium material, however it is understood that the DNA protective activity against H2O2 damage is more closely related to the enzymatic activities that continue effectively in the cell. As a matter of fact, it is known that hydrogen peroxide is converted to water by the enzymatic activities of GPx and CAT in a cell [6].…”

“…Stress or environmental pollution components such as smoke and heavy metals are known to cause oxidative stress associated with cancer or neurodegenerative diseases by increasing the concentration of the reactive oxygen species (ROS) in a cell [6][7][8]. To protect from the aforementioned diseases, the reduction of heavy metals or the scavenging of free radicals are important for the continuation of life and plants are one of the most reliable sources for this.…”

Some biological properties of ethanol extract prepared from the aerial parts of Scutellaria albida L. subsp. condensata (Rech.f.) J.R. Edm.

“…It is clear that in this study, the antimicrobial effect at 150 µL extract volume was similar to ampicillin/sulbactam and amikacin or higher than them. Some factors such as adverse environmental conditions and the misuse of medications cause oxidative stress and various metabolic diseases by increasing cellular radical concentration [31,32]. Moreover, the radicals can transform the form I structure of DNA into form II and form III by disrupting the double helix of DNA [33].…”

WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy

“…The staining of the intra-cellular lipid structures (lipid drops) remains intense. This effect may be associated with the influence of the cefazolin on the antioxidant system of a cells, which leads to an increase in reactive oxygen species inside the cells, which in turn causes lipid peroxidation in membranes [70]. However, when incubated with free cefazolin at a concentration of 10 mg/mL, all cells in the view field become rounded, detaching from the substrate, which indirectly indicates the negative effect of the antibiotic.…”