The effects of ammonia-N stress on immune parameters, antioxidant capacity, digestive function, and intestinal microflora of Chinese mitten crab, Eriocheir sinensis, and the protective effect of dietary supplement of melatonin

Yang, Xiaozhen; Shi, Aoya; Song, Yameng; Niu, Chao; Yu, Xiaowen; Shi, Xingliang; Pang, Yangyang; Ma, Xueli; Cheng, Yongxu

doi:10.1016/j.cbpc.2021.109127

Cited by 12 publications

(10 citation statements)

References 59 publications

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“…There is no doubt that the feeds have a positive effect on the growth and immunity of crabs. Our previous work also found that feeds (Melatonin added) have a strong immune regulation and anti-stress effect on crabs (Song et al, 2021;Yang et al, 2021;Zhang et al, 2019b). However, it is not clear whether single factor feeds have an impact on E. nuttallii and water quality.…”

Section: Discussionmentioning

confidence: 93%

“…The E. nuttallii was collected from the pond at Chongming Research Base of Shanghai Ocean University (Shanghai, China). Before the experiment, the E. nuttallii was cleaned gently by hand to remove epiphytic algae (Luo et al, 2020), and then all the plants were adapted to the laboratory conditions in the plastic water tank (56 × 42 × 31 cm) for one week (Yang et al, 2021). During the adaptation period, kept the water depth at 16 cm, pH at 7.8 ~ 8.2, the temperature at 24°C ~ 25°C, and kept it for 16‐h/8‐h bright and dark times.…”

Section: Methodsmentioning

confidence: 99%

“…At present, many studies have confirmed that melatonin (MT) can improve plant resistance and regulate seed germination, seedling growth, flowering and senescence in many plants (Wang, Sun, Chang, et al, 2013;Zhang et al, 2014Zhang et al, , 2015. It has been also shown that MT supplementation in feeds causes a wide range of positive effects in E. sinensis, including survival, immune response, digestive function and gut microbiota in E. sinensis (Song et al, 2021;Yang et al, 2021;Zhang et al, 2019b). However, there is a lack of research on the effects of MT feeds on physiological indexes of aquatic plants.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we studied whether feeds directly affected E. nuttallii and water quality in laboratory conditions. In addition, our previous studies have shown that melatonin supplementation in feeds protects E. sinensis from oxidative damage and immune system caused by acute hypoxia, ammonia‐N toxicity and deltamethrin (Song et al, 2021; Yang et al, 2021; Zhang et al, 2019b). Therefore, we also studied the effect of feed type (whether MT was added to the feed) on E. nuttallii and water quality in this experiment.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin feeds promoted the growth of Elodea nuttallii by chlorophyll, devoid of the adverse effects on water quality

Yang

Niu

et al. 2022

Aquaculture Research

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In the ecological culture of the Eriocheir sinensis in China, the coexistence of feeds fed for crabs and Elodea nuttallii is a common pattern in ponds. The objective of this study, for the first time, was to evaluate the effects of feeds (Melatonin added) on E. nuttallii growth and water quality under the artificial cultivation condition and avoid the influence of complex ecological factors. According to the characteristics of E. nuttallii growth, the optimum culture conditions (light intensity, branch type and culture time) were firstly explored in the laboratory and then chosen as culture conditions for feed experiment. The results showed that the 4500lux, Branch I (with about 1 cm visible branch), and 14-day culture were selected as the optimum E. nuttallii culture conditions in the laboratory. Fresh weight, weight growth rate, relative growth rate and height in MT feeds group were significantly higher than those in the basic feeds and no feeds groups (p < 0.05). Chlorophyll b contents in the MT feeds group were significantly higher than that in the basic feeds group (p < 0.05). Total nitrogen, total phosphorus, ammonia nitrogen and nitrite nitrogen contents in MT feeds group were not significantly higher than that in the basic feeds group (p > 0.05). The results indicated that residual feeds containing melatonin in crab ponds boost the growth of E. nuttallii to some extent and without negative effects on water quality. The results will help to lay a foundation for clarifying the relationship between feeds and aquatic plants and water quality in E. sinensis aquaculture.

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Melatonin feeds promoted the growth of Elodea nuttallii by chlorophyll, devoid of the adverse effects on water quality

Yang

Niu

et al. 2022

Aquaculture Research

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“…By comparing the results of intestinal microflora under different conditions, significant increments in the abundance of Proteobacteria and a significant decrease in the abundance of Fusobacteria and the ratio of Firmicutes/Bacteroidetes were caused by excessively high carbonate alkalinity (15 mmol/L) and excessively low dietary phosphorus supply (3.6 g/kg). Generally, Proteobacteria occurs an abnormal surplus in certain conditions, such as hypoxia stress for cobia ( Rachycentron canadum ) ( 57 ), ammonia-N stress for Chinese mitten crab ( Eriocheir sinensis ) ( 58 ), excessively high salinity for yellow drum ( Nibea albiflora ) ( 59 ), excessive dietary protein for Songpu mirror carp ( 5 ), and excessive dietary fish oil for rainbow trout ( O. mykiss ) ( 55 ), thereby resulting in inflammatory intestinal disease. Hence, the variation tendency of the Proteobacteria abundance should need to be focused on when environmental conditions and feed nutrition have been sharply changed.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus Nutrition in Songpu Mirror Carp (Cyprinus carpio Songpu) During Chronic Carbonate Alkalinity Stress: Effects on Growth, Intestinal Immunity, Physical Barrier Function, and Intestinal Microflora

Fan

et al. 2022

Front. Immunol.

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Carbonate alkalinity is a major environmental stress factor affecting aquatic feed configuration, which easily causes oxidative stress and hypoimmunity for fish. Hence, the purpose of the study is to assess the potential effect of phosphorus on growth, intestinal oxidation resistance, physical barrier function, and microflora for Songpu mirror carp (Cyprinus carpio Songpu) (initial average weight of 2.95 ± 0.21 g) reared at the high-concentration carbonate alkalinity environment. A two-factor, three-level (2 × 3) design was applied, in which diets with three different phosphorus levels (3.6, 7.0, and 10.5 g/kg dry matter) were randomly assigned to 0 and 15 mmol/L carbonate alkalinity groups with three replicate aquariums. After the 8-week trial, we found that weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), and lipase and amylase activities in the intestine significantly (p < 0.05) declined with increasing carbonate alkalinity. Carbonate alkalinity of 15 mmol/L significantly reduced glutathione peroxidase (GSHPx) activities in the intestine (p < 0.05). The relative expressions of nuclear factor (erythroid-derived 2)-like 2 (Nrf2), glutathione peroxidase 1a (GPX1a), Clautin3, Clautin11, and tumor necrosis factor β (TNF-β) in the intestine were markedly downregulated by increasing carbonate alkalinity levels (p < 0.05), whilst the relative expressions of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in the intestine were markedly upregulated (p < 0.05). At the 15 mmol/L carbonate alkalinity treatment, Songpu mirror carp suffer from hypoimmunity status with failed digestion, antioxidant, inflammation, and immune response, thereby inducing impaired growth. Additionally, significant increments in the abundance of Proteobacteria and a significant decrease in the abundance of Fusobacteria and the Firmicutes/Bacteroidetes ratio were caused due to excessively high carbonate alkalinity (15 mmol/L) and excessively low dietary phosphorus supply (3.6 g/kg). Collectively, 7.0 g/kg dietary phosphorus supplementation was effective in promoting intestinal antioxidant enzyme activities and the corresponding gene expression via the Keap1-Nrf2 signaling pathway and in enhancing intestinal immunity by upregulating anti-inflammatory and downregulating pro-inflammatory genes. Appropriate dietary phosphorus supply could promote the formation of beneficial microflora in freshwater, and it has the potential ability to transfer the adverse effect of carbonate alkalinity stress to the structural composition of intestinal microflora. Hence, consideration should be given to suitable phosphorus supply for fish under the chronic carbonate alkalinity stress.

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Application of physicochemical techniques to the removal of ammonia nitrogen from water: a systematic review

Zhou,

Wang,

Zeng

et al. 2024

Environ Geochem Health

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The effects of ammonia-N stress on immune parameters, antioxidant capacity, digestive function, and intestinal microflora of Chinese mitten crab, Eriocheir sinensis, and the protective effect of dietary supplement of melatonin

Cited by 12 publications

References 59 publications

Melatonin feeds promoted the growth of Elodea nuttallii by chlorophyll, devoid of the adverse effects on water quality

Melatonin feeds promoted the growth of Elodea nuttallii by chlorophyll, devoid of the adverse effects on water quality

Phosphorus Nutrition in Songpu Mirror Carp (Cyprinus carpio Songpu) During Chronic Carbonate Alkalinity Stress: Effects on Growth, Intestinal Immunity, Physical Barrier Function, and Intestinal Microflora

Application of physicochemical techniques to the removal of ammonia nitrogen from water: a systematic review

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