The effects of aging, diabetes mellitus, and antiplatelet drugs on growth factors and anti-aging proteins in platelet-rich plasma

Tian, Ju; Lei, Xiao; Li, Xuan; Tang, Jian Bing; Cheng, Biao

doi:10.1080/09537104.2018.1514110

Cited by 33 publications

(28 citation statements)

References 50 publications

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“…These molecules modulate angiogenesis, remodel the extracellular matrix, and affect the recruitment, proliferation, and differentiation of stem cells (Etulain et al, 2018). For this reason, use of PRP to promote tissue growth and repair has found application in several fields of regenerative medicine including orthopedics, dental and plastic surgery, ophthalmology, and dermatology (Tian et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman’s Syndrome

et al. 2020

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Asherman's syndrome (AS) is characterized by intrauterine adhesion or fibrosis resulting from damage to the endometrium, often leading to amenorrhea, infertility, or recurrent pregnancy loss. Although various therapeutic strategies for AS have been proposed, the options remain limited. New strategies such as bone marrow-derived mesenchymal stem cell (BM-MSC) therapy aim to potentiate the intrinsic capacity of endometrial regeneration. However, BM-MSC therapy has not been widely adopted mainly because it involves invasive and expensive procedures such as bone marrow biopsy and cell storing. On the other hand, platelet-rich plasma (PRP) is considered safe and affordable because it involves the less invasive procedure of blood collection from peripheral veins to produce PRP. To assess the effectiveness of human PRP infusion for endometrial regeneration, we established a murine model of injury-induced AS and evaluated endometrial morphology, expression of fibrosis-related factors, implantation sites (IS), and pregnancy outcomes associated with human PRP treatment. We found that treatment with human PRP was associated with improved endometrial morphology, reduced degree of fibrosis, and down-regulated expression of fibrosis-related factors in murine model of AS. Furthermore, human PRP treatment was associated with a higher number of IS and live-births. Our results suggest that human PRP treatment may become a valuable strategy to promote the regeneration of damaged endometrium and thus improve fertility and pregnancy outcomes in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman’s Syndrome

et al. 2020

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“…Overall, 8 12,14,16,26,27,30,32,36 of 15 studies found a decrease in growth factors or mitogenesis. Two studies 1,2 using CaCl 2 alone and 4 18,23,29,34 of 5 studies investigating TBN alone for PRP activation found no effect on growth factor production or mitogenesis with antiplatelet or anti-inflammatory medications.…”

Section: Discussionmentioning

confidence: 99%

“…26,36 Of the studies that analyzed PRP, 2 used calcium chloride (CaCl 2 ), 1,2 5 used TBN, 12,18,23,29,34 3 used collagen, 14,16,30 2 used adenosine diphosphate (ADP), 14,32 1 used AA, 12 and 1 assessed both TBN and calcium gluconate as activating agents. 27 All studies were evaluated using a modified 27-item Downs and Black checklist. 7 We elected to use a simple 0 or 1 score for the power analysis, with papers receiving 1 point if they included power calculations showing sufficient sample size.…”

Section: Methodsmentioning

confidence: 99%

“…In their 2014 publication, Anitua and colleagues 2 measured platelet-derived growth factor (PDFG) AB, vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), clotting time, and fibroblast proliferation in elderly (>60 years old) controls (no medications) against patients using chronic 100 mg/d aspirin (acetylsalicylic acid [ASA]); varying doses of acenocoumarol; a vitamin K antagonist; or 1500 mg/d glucosamine sulfate, a cartilage precursor often taken as an over-the-counter supplement. 2 13 Takehara et al 26 14 Anitua et al 1 13 Tian et al 27 20 Jagroop et al 11 19…”

Section: Effects Of Antiplatelet Medications With Cacl 2 -Dependent Amentioning

confidence: 99%

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Effects of Antiplatelet and Nonsteroidal Anti-inflammatory Medications on Platelet-Rich Plasma: A Systematic Review

Frey

Yeh

Jayaram

2020

Orthopaedic Journal of Sports Medicine

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Background: Platelet-rich plasma (PRP) has wide applications in orthopaedic care. Its beneficial effects are attributed to the growth factor profile from the platelet secretome. In theory, these effects would be diminished by medications that inhibit platelet activation and/or the subsequent release of growth factors. Purpose: To determine whether commonly used antiplatelets, nonsteroidal anti-inflammatory drugs (NSAIDs), or anticoagulant medications affect platelet growth factor release in PRP. Study Design: Systematic review; Level of evidence, 2. Method: A systematic review of the literature related to antiplatelet, anti-inflammatory, and anticoagulant drugs was performed following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. We used the Downs and Black objective quality scoring system. The literature search consisted of PubMed and Cochrane Library databases. Search terms consisted of 1 item selected from “platelet-rich plasma,” “platelet-derived growth factor,” and “platelet-rich plasma AND growth factor” combined with 1 item from “antiplatelet,” “aspirin,” “anticoagulant,” and “NSAID.” Only studies published within the past 25 years were included. Results: A total of 15 studies met the inclusion criteria: 7 studies detected no significant decrease in growth factors or mitogenesis, whereas 6 detected a decrease with antiplatelet agents, 1 detected mixed results with an antiplatelet agent, and 1 had mixed results with an antiplatelet agent/vasodilator. In terms of PRP activation, all 3 studies assessing collagen, the 2 studies analyzing adenosine diphosphate alone, and the 1 study investigating arachidonic acid found a decrease in growth factor concentration. Conclusion: Antiplatelet medications may decrease the growth factor release profile in a cyclooxygenase 1– and cyclooxygenase 2–dependent manner. Eight of 15 studies found a decrease in growth factors or mitogenesis. However, more studies are needed to comprehensively understand antiplatelet effects on the PRP secretome.

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“…Clearly, a detailed study of the structure and function of platelets in the elderly is necessary in order to fully understand the effects of using autologous PCs for their treatment. Our previous research showed that the concentrations of several bioactive substances in PRP decrease with age …”

Section: Discussionmentioning

confidence: 99%

Investigating the effect of age on platelet ultrastructure using transmission electron microscopy

Tian

Cheng

Chen

et al. 2019

International Wound Journal

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In the present study, the age‐ and sex‐related differences in platelet ultrastructure were investigated using transmission electron microscopy (TEM). A total of 15 healthy volunteers were grouped according to age, with 5 people in each of the following groups: young group (25‐45 years), middle‐aged group (46‐65 years), and old‐aged group (> 65 years). In the TEM micrographs, the internal components, specifically the α‐granules, dense granules, and lysosomal granules, of 20 platelets were counted for each group. Two‐way analysis of variance of age and sex variance was used to compare the results. The ultrastructure of the platelets in the old‐aged group was observed to be quite different from those of the young and middle‐aged groups. Specifically, with ageing, the platelet membrane becomes more irregular in shape and non‐smooth, and multiple platelet membrane ruptures are observed. Furthermore, the pseudopodia and protuberances become more numerous and slender, and the number of α‐granules is significantly reduced. These morphological changes indicate that ageing may affect the function of platelets, which in turn affects the efficacy of platelet concentrates. Thus, the effects of age should be considered when using platelet concentrates prepared from elderly autologous blood.

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The effects of aging, diabetes mellitus, and antiplatelet drugs on growth factors and anti-aging proteins in platelet-rich plasma

Cited by 33 publications

References 50 publications

Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman’s Syndrome

Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman’s Syndrome

Effects of Antiplatelet and Nonsteroidal Anti-inflammatory Medications on Platelet-Rich Plasma: A Systematic Review

Investigating the effect of age on platelet ultrastructure using transmission electron microscopy

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