The effects of age and lifestyle factors on the accumulation of cytogenetic damage as measured by chromosome painting

Ramsey, Marilyn J.; Moore, Dan H.; Briner, Jane; Lee, Denise A.; Olsen, Letha; Senft, Jami R.; Tucker, James D.

doi:10.1016/0921-8734(95)00015-x

Cited by 261 publications

(158 citation statements)

References 53 publications

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“…[4,6,10,16,17,19,38,51]. However, in the present study, we found an increase only in the exposed women, probably because the number of individuals in our sample was too small to separate them by age bracket.…”

Section: Discussioncontrasting

confidence: 55%

“…The relationship of age with a decline in the efficiency of the DNA repair processes and the accumulation of mutations due to adverse endogenous and exogenous conditions results in an increasing level of DNA damage, reflected on the cytogenetic level by an increase in the frequency of chromosome aberrations [4,38,48,51] and MN in the peripheral blood lymphocytes of elderly individuals [6,7,16,17].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown an association between chronological age and aneuploidy, and the most frequent loss of the X and Y chromosomes in females and males respectively [4,5,38].…”

Section: Discussionmentioning

confidence: 99%

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Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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Exposure to certain chemical agents causes damage to the genetic material. There is controversy about the genotoxic and/or mutagenic effect caused by acute or chronic anesthetics exposure. The aim of this research was to assess and compare the frequency of micronuclei (MN) in professionals working in operating rooms and other hospital areas. The genotoxic and cytotoxic effects were evaluated in lymphocytes using the micronucleus test and the proliferative index, respectively. We examined peripheral blood lymphocyte cultures from 30 operating room professionals (exposed group) and 30 professionals non-exposed in other hospital areas of the same hospital (control group). There was no statistically significant difference in proliferative index between the groups. Nevertheless, there was an increase in MN frequency in binucleated (BN) cells in the exposed group (p=0.0003) compared to the control group. Moreover, there was a statistically significant difference between genders (p=0.0187), and the frequency of MN in BN cells from women was higher than in men. Therefore, gender influenced the frequency of MN. The age and period of working time in an operating room influenced the MN frequency only in women professionals. Thereby, there was a genotoxic effect in occupationally exposed professionals, and the micronucleus test could be used in the biomonitoring of human populations for evaluating the risk of developing cancer.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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“…For example, an increase in chromosomal aberrations has been observed in aging mice (30,31). Additionally, aneuploidy translocations and end-to-end fusions have been shown to be higher in peripheral blood lymphocytes and fibroblasts of the elderly than in younger individuals (32)(33)(34). Furthermore, splenic and hepatic DNA from different transgenic mice showed increased spontaneous mutation with age (35) and a highly elevated mutation frequency was also observed in the lymphocytes of senescence-accelerated mice (36).…”

Section: Discussionmentioning

confidence: 99%

Senescence-Dependent MutSα Dysfunction Attenuates Mismatch Repair

Chang¹,

Jin

Yoon

et al. 2008

Molecular Cancer Research

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DNA damage and mutations in the genome increase with age. To determine the potential mechanisms of senescence-dependent increases in genomic instability, we analyzed DNA mismatch repair (MMR) efficiency in young and senescent human colonic fibroblast and human embryonic lung fibroblast. It was found that MMR activity is significantly reduced in senescent cells. Western blot and immunohistochemistry analysis revealed that hMSH2 and MSH6 protein (MutSA complex), which is a known key component in the MMR pathway, is markedly down-regulated in senescent cells. Moreover, the addition of purified MutSA to extracts from senescent cells led to the restoration of MMR activity. Semiquantitative reverse transcription-PCR analysis exhibited that MSH2 mRNA level is reduced in senescent cells. In addition, a decrease in E2F transcriptional activity in senescent cells was found to be crucial for MSH2 suppression. E2F1 small interfering RNA expression reduced hMSH2 expression and MMR activity in young human primary fibroblast cells. Importantly, expression of E2F1 in quiescent cells restored the MSH2 expression as well as MMR activity, whereas E2F1-infected senescent cells exhibited no restoration of MSH2 expression and MMR activity. These results indicate that the suppression of E2F1 transcriptional activity in senescent cells lead to stable repression of MSH2, followed by a induction of MutSA dysfunction, which results in a reduced cellular MMR capacity in senescent cells. (Mol Cancer Res 2008;6(6):978 -89)

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“…Preparation of cell cultures and slides was performed using standardised methods (28,29) . Chromosomes 1, 2 and 4 were painted red, and chromosomes 3, 5 and 6 were simultaneously painted green.…”

Section: Assay For Chromosome Translocationsmentioning

confidence: 99%

High dietary niacin intake is associated with decreased chromosome translocation frequency in airline pilots

Yong

Petersen

2010

Br J Nutr

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Experimental studies suggest that B vitamins such as niacin, folate, riboflavin, vitamin B 6 and vitamin B 12 may protect against DNA damage induced by ionising radiation (IR). However, to date, data from IR-exposed human populations are not available. We examined the intakes of these B vitamins and their food sources in relation to the frequency of chromosome translocations as a biomarker of cumulative DNA damage, in eighty-two male airline pilots. Dietary intakes were estimated by using a self-administered semi-quantitative FFQ. Translocations in peripheral blood lymphocytes were scored by using fluorescence in situ hybridisation whole-chromosome painting. Negative binomial regression was used to estimate rate ratios and 95 % CI, adjusted for age and occupational and lifestyle factors. We observed a significant inverse association between translocation frequency and dietary intake of niacin (P¼0·02): adjusted rate ratio for subjects in the highest tertile compared with the lowest tertile was 0·58 (95 % CI 0·40, 0·83). Translocation frequency was not associated with total niacin intake from food and supplements as well as dietary or total intake of folate, riboflavin or vitamin B 6 or B 12 . However, the adjusted rate ratios were significant for subjects with $median compared with ,median intake of whole grains (P¼ 0·03) and red and processed meat (P¼0·01): 0·69 (95 % CI 0·50, 0·96) and 1·56 (95 % CI 1·13, 2·16), respectively. Our data suggest that a high intake of niacin from food or a diet high in whole grains but low in red and processed meat may protect against cumulative DNA damage in IR-exposed persons.

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The effects of age and lifestyle factors on the accumulation of cytogenetic damage as measured by chromosome painting

Cited by 261 publications

References 53 publications

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Senescence-Dependent MutSα Dysfunction Attenuates Mismatch Repair

High dietary niacin intake is associated with decreased chromosome translocation frequency in airline pilots

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