The effects of aerobic exercise frequencies on liver fibrosis, α-fetoprotein and cytokeratin 19 in experimental type 2 diabetes-induced rats: an immunohistochemistry study

Uslu, Serap; Alaca, Nuray; Kılıç, Kubilay Doğan; Uysal, Ayşegül; Kurtel, Hızır

doi:10.1080/10520295.2018.1517898

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…FBG was high at the end of 8th week in the SD group while decreases were observed in all exercise groups. These results were consistent with previous studies on the effects of exercise on FBG 8,13 . In addition, exercise is known to increase insulin sensitivity 8 .…”

Section: Discussionsupporting

confidence: 94%

“…We found that, all frequencies of exercise have beneficial effects on these parameters and none one of the exercises is superior to the others 8 . Our observations on muscle and liver tissue 13 lead us to hypothesize that intermittent exercise approach can also be benefial on the outcome of heart and kidney injury induced by T2DM. Furthermore, for the abovementioned reasons there may be a link between VEGF metabolism and experimental T2DM model and if so, benefits of intermittent exercise may be related with the altered VEGFstate.…”

Section: Introductionmentioning

confidence: 81%

“…All histomorphometric analysis were performed after the images were transferred by light microscope (Olympus BX53, bright-field microscope, America Inc., USA) into a computer. Serial sections in each rats were quantified at a magnification of X 40 for each section after processing by a semiautomatic digitizer The University of Texas Health Science Center At Santorino (UTHSCSA Image Tool for Windows Version 1.28) 13 .…”

Section: Thickness Of Basement Membranementioning

confidence: 99%

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Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Alaca¹,

Uslu²,

Başdemir³

et al. 2019

MMJ

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Objective: Exercise has many beneficial effects in the treatment and prevention of Type 2 Diabetes Mellitus (T2DM). The aim of this study was to evaluate the effect of physical activities with different frequencies performed within a total total duration of one week on the heart and kidney tissues and vascular endothelial growth factor (VEGF) expressions in experimental T2DM model. Method: Rats (n: 30) were divided into sedentary control (SC), sedentary T2DM (SD), T2DM and continuous exercise (DEc, 30 min/day, 5 days/week), T2DM and short bouts exercise (DEsb, 3x10 min/day, 5 days/week), T2DM and weekend warrior exercise (DEww, 35+40 min/day, 2 days/week) groups. Rats were administered streptozotocin (65 mg/kg) and nicotinamide (110 mg/kg) through intraperitoneal route. After 6-weeks of swimming exercise (total duration 150 min/week), biochemical analyzes were performed to measure oral glucose tolerance test, insulin sensitivity and cytokines. Histopathological and immunohistochemical analyses [VEGF, capillary density, Transforming growth factor beta (TGF-β)] were performed in heart and kidney tissues. Results: Compared with sedentary T2DM rats, significant improvements were observed in all exercise groups in terms of blood glucose level, insulin sensitivity, capillary density in heart tissue, VEGF expressions in tissues, TGF-β expressions in kidney tissue and all histopathological analysis (p<0.05). Conclusion: This study shows that physical activity at various frequencies may significantly ameliorate harmful effects of T2DM on heart and kidney tissue without significant differences between exercise frequencies, provided that the total duration of aerobic exercise remains the same (150 min/week).

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 81%

Section: Thickness Of Basement Membranementioning

confidence: 99%

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Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Alaca¹,

Uslu²,

Başdemir³

et al. 2019

MMJ

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“…Many studies have shown that in addition to pancreatic β-cell damage, STZ can also cause increased serum AFP and liver a-SMA, fibrosis, and necrosis in rats and mice [ 30 , 31 ]. The present data demonstrate that (1) specifically inhibiting the activity of DPP4 in hepatocytes by sitagliptin contributes to decreased activation of NFκB pathway and oxidative stress, as well as cell apoptosis under diabetic conditions, and (2) the ROS cleaning function of sitagliptin promotes the deactivation of NFκB pathway; moreover, (3) sitagliptin can attenuate STZ-induced chronic liver injury and oxidative stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

Dipeptidyl peptidase-4 (DPP4) inhibitor sitagliptin alleviates liver inflammation of diabetic mice by acting as a ROS scavenger and inhibiting the NFκB pathway

Wang

Zhu

et al. 2021

Cell Death Discov.

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As a common chronic metabolic disease, the development of diabetes mellitus (DM) may also be accompanied by liver damage and inflammatory disorders. Sitagliptin is an inhibitor of dipeptidyl peptidase-4 (DPP4, also known as CD26), which is clinically used for DM treatment. However, the mechanism of sitagliptin’s efficiency in liver diseases is largely unknown. In this study, mice suffering from streptozotocin (STZ) exhibit elevated liver DPP4 expression and activity, as well as inflammatory and chronic liver injury phenotype, whereas specifically inhibiting the activity of DPP4 in mouse liver tissues and hepatocytes by sitagliptin contributes to decreased cytokines, oxidative stress, cell apoptosis, and inflammation in STZ-induced diabetic mice. Moreover, sitagliptin reduced TNFα or LPS-induced cellular reactive oxygen species (ROS) level, cell apoptosis, and protein expression in the NFκB signaling pathway in HepG2 cells or primary mouse hepatocytes. Altogether, our study confirms that sitagliptin may protect liver tissue by alleviating ROS production and NFκB signaling activation, providing a putative mechanism for preventing the development of diabetic liver disease.

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“…A avaliação do perfil lipídico foi realizada uma semana antes do início do treino, na 8ª semana de treino e na 13ª semana, uma semana após o término do treino. Os valores de colesterol total foram categorizados como: maiores do que 149 mg/dL e menores do que 149 mg/dL e os valores dos triglicerídeos foram apresentados em números absolutos (USLU et al, 2018).…”

Section: Grupos Experimentaisunclassified

Efeito protetor do treino aeróbico em ratos submetidos à dieta hiperlipídica e normolipídica mensurados pela análise do perfil lipídico e do processo inflamatório tecidual / Protective effect of aerobic training in rats submitted to hyperlipidic and normolipidic diets measured by the analysis of lipid profile and tissue inflammatory process

Magalhães¹,

Filho²,

Mariolani³

et al. 2021

Braz. J. Hea. Rev.

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Avaliar o efeito protetor do treino aeróbico em ratos submetidos a dieta hiperlipídica e padrão levando-se em consideração o ganho da massa corporal, o acúmulo do tecido adiposo visceral e o perfil lipídico sérico. Métodos: Trinta e seis ratos machos Wistar foram divididos nos grupos Dieta Padrão e Dieta Hiperlipídica, cada grupo com subgrupos sedentário e treinados com o mesmo protocolo aeróbico. O consumo calórico foi medido semanalmente. O colesterol total e os triglicerídeos séricos foram medidos na fase pré-treino, 8ª e 13ª semana. Logo após a eutanásia foi mensurada a massa corporal, dosada a lipoproteína de alta densidade (HDL), coletado o tecido adiposo para cálculo do percentual relativo à massa corporal e o músculo sóleo para análise morfométrica. Na análise estatística foram utilizados os Teste Kruskall-Wallis e Wilcoxon, adotando p<0,05. Resultados: Sem gerar diferenças nas massas corporal e do sóleo entre os subgrupos (p>0,05), o treino aeróbico reduziu o colesterol total em ambos os grupos (p<0,05). No grupo Hiperlipídico, o treino aeróbico aumentou o trofismo muscular (p<0,05). No grupo Padrão, o treino aeróbico aumentou o HDL, diminuiu o tecido adiposo relativo e a inflamação muscular (p<0,05). O grupo Hiperlipídico apresentou menor consumo calórico, maior nível de triglicerídeos séricos e maior quantidade de tecido adiposo total e relativo comparado ao grupo Padrão (p<0,05). Conclusão: O treino aeróbico reduziu o colesterol total em ambos os grupos, gerou hipertrofia muscular no grupo Hiperlipídico e reduziu o tecido adiposo relativo, a inflamação muscular e aumentou o HDL no grupo Padrão.

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The effects of aerobic exercise frequencies on liver fibrosis, α-fetoprotein and cytokeratin 19 in experimental type 2 diabetes-induced rats: an immunohistochemistry study

Cited by 9 publications

References 34 publications

Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Dipeptidyl peptidase-4 (DPP4) inhibitor sitagliptin alleviates liver inflammation of diabetic mice by acting as a ROS scavenger and inhibiting the NFκB pathway

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