The effects of additive solution pH and metabolic rejuvenation on anaerobic storage of red cells

Abstract

Extended RBC storage may be achieved using anaerobic conditions combined with low-pH AS and rejuvenation during storage.

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

“…This is supported by a previous report indicating that 2,3-BPG is increased but ATP is decreased in erythrocytes stored in an alkaline additive solution at pH 8.7 . Though pH has been studied as a target for developing novel blood storage solutions (de Korte et al, 2008;Yoshida et al, 2008), it is difficult to overcome the trade-off between maintaining 2,3-BPG or ATP levels.…”

Association between red blood cell transfusions and development of non-Hodgkin lymphoma: a meta-analysis of observational studies

“…32 RBC components are stored with a variety of solutions, which can potentially affect the aging process of the RBCs. 33 Among the most common solutions are CPD (trisodium citrate, dextrose, and disodium phosphate) and acidic additive solutions. However, most of the studies did not specify the type of solution the RBC transfusions contained.…”

Blood Aging, Safety, and Transfusion: Capturing the “Radical” Menace

“…Second, cell senescence and changes in RBC reducing power (i.e., depletion of endogenous antioxidants) can accelerate the rate of Hb auto-oxidation and elevate the concentration of intracellular ROS. Third, the level of molecular O 2 within the RBC unit that is available for redox reactions can also be reduced (113) [For recent discussion of auto-oxidation reactions of Hb and its relationship to oxygen, see (91)]. …”

“…Finally, the storing vehicle, including the blood bag and the additive solutions, may also alter the Hb oxidative state (113).…”