The Effects of Acidosis on eNOS in the Systemic Vasculature: A Focus on Early Postnatal Ontogenesis

Gaynullina, Dina; Tarasova, O. S.; Shvetsova, Anastasia A.; Борзых, А. А.; Schubert, Rudolf

doi:10.3390/ijms23115987

Cited by 6 publications

(3 citation statements)

References 118 publications

(181 reference statements)

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“…In addition, high glucose causes damage to endothelial cells by increasing TOS concentrations and impairs vascular responses ( Stocker and Keaney, 2004 ; Tota et al, 2021 ). This deterioration in vascular responses has also been attributed to NO-dependent mechanisms ( Alp et al, 2003 ; Cai et al, 2005 ; Gaynullina et al, 2022 ). Therefore, we measured eNOS levels in the high glucose group and found a significantly decreased eNOS, parallel with other studies ( Han et al, 2014 ; Kassan et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of sulfasalazine on endothelium-dependent vascular response by the activation of Nrf2 signalling pathway

Sonmez

Shahzadi²,

Kose³

et al. 2022

Front. Pharmacol.

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Background: Diabetes mellitus leads to endothelial dysfunction and accumulation of oxygen radicals. Sulfasalazine-induced Nrf2 activation reduces oxidative stress in vessels. Thus, in the present study, we investigated the effects of sulfasalazine on endothelial dysfunction induced by high glucose. We also ascribed the underlying mechanism involved in glucose-induced endothelial dysfunction.Methods: For this experiment we used 80 Wistar Albino rats thoracic aorta to calculate the dose response curve of noradrenaline and acetylcholine. Vessels were incubated in normal and high glucose for 2 h. To investigate glucose and sulfasalazine effects the vessels of the high glucose group were pre-treated with sulfasalazine (300 mM), JNK inhibitor (SP600125), and ERK inhibitor (U0126) for 30 min. The dose response curve was calculated through organ bath. The eNOS, TAS, TOS, and HO-1 levels were estimated by commercially available ELISA kits.Results: In the high glucose group, the Emax for contraction was significantly higher (p < 0.001), and Emax for relaxation was lower than that of control. These functional changes were parallel with the low levels of eNOS (p < 0.05). High glucose vessel treated with sulfasalazine showed low Emax value for contraction (p < 0.001) however, the Emax for relaxation was significantly high (p < 0.001) when compared to high glucose group. In the JNK group, Emax for contraction and relaxation was inhibited (p < 0.001) compared to sulfasalazine treated vessels. HO—1 enzyme levels were significantly low (p < 0.01) with sulfasalazine but higher with ERK inhibitor (p < 0.05).Conclusion: High glucose induced endothelial dysfunction and sulfasalazine reduced damage in high glucose vessels by activating eNOS, antioxidant effect through HO-1 enzymes and particularly inducing Nrf2 via the ERK and JNK pathways.

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Section: Discussionmentioning

confidence: 99%

Effect of sulfasalazine on endothelium-dependent vascular response by the activation of Nrf2 signalling pathway

Sonmez

Shahzadi²,

Kose³

et al. 2022

Front. Pharmacol.

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“…The body's response to the hypoxaemic state either acute or chronic, is linked to an increase in minute ventilation leading to a steady increase of hypocapnia, as the CO2 diffuses through tissues faster than O2. Though the clinical significance of this loop still remains to clarify, the whole scenario suggests that this condition may generate a steady immune dysfunction due to a subtle inflammatory process that progress over the years almost unnoticed, typical patterns that vividly recall the the ABDs [22][23][24].…”

Section: Extracellular Acidosis and The Immunity Clinical And Physiol...mentioning

confidence: 99%

The Importance of Arterial Blood Gas Analysis as a Systemic Diagnosis Approach in Assessing and Preventing Chronic Diseases, From Emergency Medicine to the Daily Practice

Balzanelli¹,

DISTRATIS²,

LAZZARO³

et al. 2023

Preprint

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Blood gas analysis is a diagnostic tool to evaluate the partial pressures of gas in blood and acid-base content. The use of blood gas analysis enables to clear understanding the respiratory, circulatory, and metabolic disorders. The arterial blood gas (ABG) explicitly analyzes blood taken from an artery, assessing the patient's partial pressure of oxygen (PaO2), carbon dioxide (PaCO2) pH (acid/base). PaO2 indicates the oxygenation status, and PaCO2 indicates the ventilation status (chronic or acute respiratory failure). PaCO2 is affected by hyperventilation characterized by rapid or deep breathing, and hypoventilation characterized by slow or shallow breathing. The acid-base balance tested by the ABG procedure measures the pH and PaCO2 directly while the use of the Hasselbach equation gives the serum bicarbonate (HCO3) and base deficit or excess. The measured HCO3is based on strong alkali that frees all CO2 in serum, including dissolved CO2, carbamino compounds, and carbonic acid the calculation uses a standard chemistry analysis giving the amount of "total CO2", the difference will amount to around 1.2 mmol/L. Though ABG is frequently ordered in emergency medicine contests for acute conditions it may also be needed in other clinical settings. The ABG analysis shows to be an exceptional diagnostic tool including the group of those diseases known as acid-base diseases (ABDs) which include a great variety of conditions as such severe sepsis, septic shock, hypovolemic shock, diabetic ketoacidosis, renal tubular acidosis, chronic respiratory failure, chronic heart failure, and diverse metabolic diseases.

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“…Interleukin (IL)-6, IL-8, angiotensin (Ang) II, and tumor necrosis factor (TNF)-α are important inflammatory factors and are abundantly expressed in the process of vascular injury. [8][9][10] Ang II, thromboxane (TX)A 2 , and ET-1 are vasoconstriction regulators, 11,12 while prostaglandin (PG)I 2 , atrial natriuretic peptide (ANP), and nitric oxide (NO) are vasodilation regulators, [13][14][15] which collectively play an important role in maintaining normal vascular ecology.…”

mentioning

confidence: 99%

A Study on Vascular Regulators as Early Biomarkers of Hand Arm Vibration Syndrome

Yang,

Chen,

et al. 2023

J Occup Environ Med

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Objectives Purpose of this study was to study the changes in expression of vascular regulators after vibration exposure and during the onset of vibration-induced white finger (VWF) and to screen for Vascular Regulatory Factors that could be used as early biomarkers of HAVS. Methods Using judgmental sampling from a Chinese factory, workers with VWF and hand-transmitted vibration exposure but without VWF were selected for research. Blood samples were taken from all subjects, and the levels of nine of the vascular regulators were measured using ELISA. Results Receiver Operating Characteristic (ROC) curve analysis was performed on nine vascular regulators to assess their diagnostic sensitivity for VWF with the following area under the curve (AUC) results: PGI2 = 0.861, ANP = 0.840. Conclusion Collectively, PGI2 and ANP exhibited the most potential for the early diagnosis of HAVS.

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The Effects of Acidosis on eNOS in the Systemic Vasculature: A Focus on Early Postnatal Ontogenesis

Cited by 6 publications

References 118 publications

Effect of sulfasalazine on endothelium-dependent vascular response by the activation of Nrf2 signalling pathway

Effect of sulfasalazine on endothelium-dependent vascular response by the activation of Nrf2 signalling pathway

The Importance of Arterial Blood Gas Analysis as a Systemic Diagnosis Approach in Assessing and Preventing Chronic Diseases, From Emergency Medicine to the Daily Practice

A Study on Vascular Regulators as Early Biomarkers of Hand Arm Vibration Syndrome

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