The Effects of a Single Cervical Inoculation of Chlamydia trachomatis on the Female Reproductive Tract of the Baboon (Papio anubis)

Bell, Jason D.; Bergin, Ingrid L.; Harris, Lisa H.; Chai, Daniel; Mullei, Isaac; Mwenda, Jason M.; Dalton, Vanessa K.; Vahratian, Anjel; LeBar, William; Zochowski, Melissa K.; Kiulia, Nicholas M.; Aronoff, David M.; Patton, Dorothy L.

doi:10.1093/infdis/jir541

Cited by 13 publications

(24 citation statements)

References 24 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 Animals were captured using baited traps and transported to the Institute for Primate Research (IPR) in Nairobi, Kenya. Animals were trapped as entire troops from areas in which they had been reported as a nuisance.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Novel Genital Alphapapillomaviruses in Baboons (Papio hamadryas Anubis) With Cervical Dysplasia

et al. 2012

View full text Add to dashboard Cite

Genital Alphapapillomavirus (αPV) infections are one of the most common sexually transmitted human infections worldwide. Women infected with the highly oncogenic genital human papillomavirus (HPV) types 16 and 18 are at high risk for development of cervical cancer. Related oncogenic αPVs exist in rhesus and cynomolgus macaques. Here the authors identified 3 novel genital αPV types (PhPV1, PhPV2, PhPV3) by PCR in cervical samples from 6 of 15 (40%) wild-caught female Kenyan olive baboons ( Papio hamadryas anubis). Eleven baboons had koilocytes in the cervix and vagina. Three baboons had dysplastic proliferative changes consistent with cervical squamous intraepithelial neoplasia (CIN). In 2 baboons with PCR-confirmed PhPV1, 1 had moderate (CIN2, n = 1) and 1 had low-grade (CIN1, n = 1) dysplasia. In 2 baboons with PCR-confirmed PhPV2, 1 had low-grade (CIN1, n = 1) dysplasia and the other had only koilocytes. Two baboons with PCR-confirmed PhPV3 had koilocytes only. PhPV1 and PhPV2 were closely related to oncogenic macaque and human αPVs. These findings suggest that αPV-infected baboons may be useful animal models for the pathogenesis, treatment, and prophylaxis of genital αPV neoplasia. Additionally, this discovery suggests that genital αPVs with oncogenic potential may infect a wider spectrum of non-human primate species than previously thought.

show abstract

Section: Methodsmentioning

confidence: 99%

“…This was an unexpected and potentially confounding finding emerging from an experimental study of Chlamydia trachomatis –induced pelvic inflammatory disease. 2 To our knowledge, genital PV infection or CIN-type changes have not been described in the baboon.…”

mentioning

confidence: 88%

Novel Genital Alphapapillomaviruses in Baboons (Papio hamadryas Anubis) With Cervical Dysplasia

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Shortly thereafter, Reacher et al described CD4 and CD8 infiltrates in chronic trachoma conjunctival biopsy specimens (13), and compelling trachoma follicle immunohistochemistry done by Abu el-Asrar et al showed conjunctival follicle architecture consisting of a prominent B cell component with addition of CD4/CD8 T cells and macrophages (14). Similarly, baboons transcervically infected with C. trachomatis serovar E and macaque salpingeal pockets repeatedly infected with C. trachomatis serovar D showed germinal center formation (follicles) that included plasma cells on routine histology of the cervix and salpingeal tissue, respectively (15,16). An immunohistochemistry study in the C. muridarum mouse model by Morrison and Morrison is a virtual case study in Trm immunobiology (1).…”

Section: Tissue-resident Memory T Cellsmentioning

confidence: 99%

Tissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity

Johnson

Brunham

2016

Infect Immun

View full text Add to dashboard Cite

bFor almost 2 decades, results from Chlamydia pathogenesis investigations have been conceptualized using a cytokine polarization narrative. Recent viral immunity studies identifying protective tissue-resident memory T cells (Trm) suggest an alternative paradigm based on localized immune networks. As Chlamydia vaccines enter the preclinical pipeline and, in the case of an attenuated trachoma vaccine, are given to human subjects, it may be useful to ask whether cytokine polarization is the appropriate framework for understanding and evaluating vaccine efficacy. In this review, we revisit C. trachomatis pathogenesis data from mice and humans using a Trm narrative and note a comfortable concordance with the Chlamydia pathogenesis literature. Chlamydia trachomatis infections of ocular and genital tract mucosal epithelia elicit host responses that include formation of submucosal lymphoid aggregates that persist at the site of infection after infectious bacteria are no longer present. In trachoma, these aggregates, called follicles, are easily visible without magnification on routine physical examination and are used to make the clinical diagnosis. Lymphoid aggregates have also been documented in uterine and cervical samples of women with C. trachomatis genital tract infections and are seen in the C. muridarum mouse model of genital tract infections (detailed below).Investigators have speculated about a role for local lymphoid aggregates in protective immunity to Chlamydia. Most presciently, Morrison and Morrison concluded that the "persistence of CD4ϩ -T-cell clusters long after infection had resolved (day 70) may provide for a readily mobilizable T-cell response by which previously infected animals can quickly respond to and control a secondary infectious challenge" (1). Strikingly, this concept is not featured in working models of Chlamydia immunopathogenesis. Instead, CD4 T cell cytokine polarization (Th1, Th2, Th17, . . .) has served as theoretical scaffolding for describing immunoprotection and immunopathology associated with infection. It is unclear whether cytokine polarization is the appropriate framework for understanding Chlamydia pathogenesis. Recent work in viral pathogenesis has identified semiautonomous tissue-localized lymphoid structures containing memory T cells as being critical for protective antiviral immunity (2, 3). The available data suggest that the tissue-resident memory T cells (Trm) in lymphoid aggregates formed in response to C. trachomatis infection play a central role in protective and pathological immune responses in the eye and genital tract. TISSUE-RESIDENT MEMORY T CELLSTrm were originally described for CD8 T cells in the setting of recurrent herpes simplex virus (HSV) reactivations. HSV infects peripheral sensory nerves at the site of infection and travels in a retrograde manner up nerve axons to set up a latent infection in the associated sensory ganglion. When HSV reactivates due to UV exposure or stress, it travels back down the nerve axon and infects the stromal cells adjacent to...

show abstract

“…Although several nonhuman primate models have been used in Chlamydia research, including the grivet monkey (126), marmoset (127), and baboon (128), the pigtailed macaque is utilized most frequently for genital research. Indeed, it is naturally infected with C. trachomatis human biovars, and the female anatomy, menstrual cycle, and vaginal microflora are akin to those in humans (129).…”

Section: Pigtailed Macaquementioning

confidence: 99%

Genital Chlamydia trachomatis: Understanding the Roles of Innate and Adaptive Immunity in Vaccine Research

Vasilevsky¹,

Greub

Nardelli-Haefliger

et al. 2014

Clin Microbiol Rev

View full text Add to dashboard Cite

SUMMARY Chlamydia trachomatis is the leading cause of bacterial sexually transmitted disease worldwide, and despite significant advances in chlamydial research, a prophylactic vaccine has yet to be developed. This Gram-negative obligate intracellular bacterium, which often causes asymptomatic infection, may cause pelvic inflammatory disease (PID), ectopic pregnancies, scarring of the fallopian tubes, miscarriage, and infertility when left untreated. In the genital tract, Chlamydia trachomatis infects primarily epithelial cells and requires Th1 immunity for optimal clearance. This review first focuses on the immune cells important in a chlamydial infection. Second, we summarize the research and challenges associated with developing a chlamydial vaccine that elicits a protective Th1-mediated immune response without inducing adverse immunopathologies.

show abstract

The Effects of a Single Cervical Inoculation of Chlamydia trachomatis on the Female Reproductive Tract of the Baboon (Papio anubis)

Cited by 13 publications

References 24 publications

Novel Genital Alphapapillomaviruses in Baboons (Papio hamadryas Anubis) With Cervical Dysplasia

Novel Genital Alphapapillomaviruses in Baboons (Papio hamadryas Anubis) With Cervical Dysplasia

Tissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity

Genital Chlamydia trachomatis: Understanding the Roles of Innate and Adaptive Immunity in Vaccine Research

Contact Info

Product

Resources

About