The effects of 4-MEI on cell proliferation, DNA breaking and DNA fragmentation

Abstract

4-Methylimidazole (4-MEI) is a color widely found in cola drinks, roasted foods, grilled meats, coffee and other foods. This study was aimed to investigate the 4-MEI effects on the cell proliferation, purifi ed circular DNA and DNA from cells of rats treated with the 4-MEI. In this study, mouse 3T3-L1 cell line was treated with 4-MEI at concentrations of 300, 450, 600 and 750 μg/mL for 24 hours and 48 hours periods, after that cytotoxic effect of the 4-MEI was studied by MTT test. Also, the effect of 4-MEI on purifi ed circular DNA (pET22b) was investigated by treating of the DNA with 4-MEI concentrations of 300, 450, 600 and 750 μg/ml. DNA was extracted from liver cells of rats that have been treated with 4-MEI doses of 25 and 50 mg/kg for 10 week and it was subjected to agarose gel electrophoreses analyses.4-MEI signifi cantly inhibited cell proliferation of 3T3-L1 cell line at highest concentration for 24 h and at all concentration for 48 h treatment time. DNA fragmentation assay showed that 4-MEI at 50 mg/kg concentration clearly produced characteristic DNA smear and no DNA laddering (200bp) was observed when mouse was exposed to 4-MEI. The results obtained from plasmid DNA damaging assay showed that 4-MEI has noeffect on the DNA, because the electrophoretic pattern of DNA treated with 4-MEI showed three bands on agarose gel electrophoresis as it was for untreated control. 4-MEI showed cytotoxic effect on 3T3-L1 cells but no effect on plasmid DNA breaking. According to DNA fragmentation assay 4-MEI has necrosis effects on mouse liver cells (Tab. 1, Fig. 4, Ref. 27). Text in PDF www.elis.sk.

Protective and genotoxic effect of food coloring

“…Edenharder and Tang, who observed the antimutagenic effect of purpurin, alizarin and other 10 anthraquinone compounds using the Ames test [21]. Similar results were obtained by Rayes who found the Cola soft drink had toxic effect on the mice testicular cells [22][23][24]. According to our and other researcher's results, some of food dyes usually induce genotoxicity.…”

The effect of doxepin on Bacillus subtilis and Pseudomonas aeruginosa

“…Saito et al [24] who found that in Chinese hamster 79 cells, bupropion hydrochloride caused DNA single strand breaks and decrease in the DNA and RNA content. In addition, Jefferson et al [25] observed that bupropion produced positive response (2 to 3 times control mutation rate) in 2 of 5 strains in the Ames bacterial mutagenicity test [26,27].…”

Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

“…After incubation, the reaction mixture was analyzed by 1 % agarose gel electrophoresis for 45 min at 110 volts. DNA bands (supercoiled, linear, and open circular) were stained with ethidium bromide and were analyzed [21]. To understand the drug's ability to break DNA in the presence of CuCl2, 0.5273 µmole bp of pET22 plasmid DNA having 0.003 µmole CuCl2 2H2O treated with a different amount of Escitalopram (0.28, 0.14, 0.014, 0.0014, 0.00014 µmole) for 1 h and were subjected to 1 % agarose gel electrophoresis.…”