The effects of 30 days resveratrol supplementation on adipose tissue morphology and gene expression patterns in obese men

Abstract

Polyphenolic compounds, such as resveratrol, have recently received widespread interest because of their ability to mimic effects of calorie restriction. The objective of the present study was to gain more insight into the effects of 30 days resveratrol supplementation on adipose tissue morphology and underlying processes. Eleven healthy obese men were supplemented with placebo and resveratrol for 30 days (150 mg per day), separated by a 4-week washout period in a double-blind randomized crossover design. A postprandial abdominal subcutaneous adipose tissue biopsy was collected to assess adipose tissue morphology and gene expression using microarray analysis. Resveratrol significantly decreased adipocyte size, with a shift toward a reduction in the proportion of large and very-large adipocytes and an increase in small adipocytes. Microarray analysis revealed downregulation of Wnt and Notch signaling pathways and upregulation of pathways involved in cell cycle regulation after resveratrol supplementation, suggesting enhanced adipogenesis. Furthermore, lysosomal/phagosomal pathway and transcription factor EB were upregulated reflecting an alternative pathway of lipid breakdown by autophagy. Further research is necessary to investigate whether resveratrol improves adipose tissue function.

Resveratrol and SRT1720 Elicit Differential Effects in Metabolic Organs and Modulate Systemic Parameters Independently of Skeletal Muscle Peroxisome Proliferator-activated Receptor γ Co-activator 1α (PGC-1α)

“…Interestingly, mice expressing a dominant negative form of CREB in WAT (24) display similar phenotypic changes as seen with RSV treatment in our study, including reduced inflammation, improved GLUT4 expression, and improved whole body glucose tolerance. Moreover, a transcription factor analysis performed in WAT from humans receiving RSV treatment predicted reduced CREB1 activity with RSV administration (41). These studies are in agreement with our findings and give credence to the theory that the repression of CREB-ATF3 axis by RSV treatment in WAT might also be relevant in humans.…”

Supplementation with high-dose trans-resveratrol improves ultrafiltration in peritoneal dialysis patients: a prospective, randomized, double-blind study

“…[15][16][17][18] In the present study, 150 and 450 mg/d transresveratrol were chosen as the two doses were suggested to be safe yet effective and tolerant enough. 19,20 We did not find any resveratrol-related adverse events, and the patients were well-tolerated at both doses. The placebo was indistinguishable by color, form, or taste from the active drug.…”

Metabolic effects of resveratrol: addressing the controversies

“…The muscle biopsies also showed that there was an increase in AMPK activity, Sirt1 level, and PGC-1a level in tissues from the resveratrol-treated group. A follow-up from this study examining adipose tissues from the subjects was recently reported [209]. This study found that the resveratrol treated subjects had lower mean adipocyte size and upregulation of expression of genes involved in adipogenesis [209].…”

“…A follow-up from this study examining adipose tissues from the subjects was recently reported [209]. This study found that the resveratrol treated subjects had lower mean adipocyte size and upregulation of expression of genes involved in adipogenesis [209]. Two subsequent studies found no effect of resveratrol on metabolic parameters.…”