The effects of 2ip and 2,4-D on rice calli differentiation

Abstract

The role of isopentenyl adenine (2ip) in cereal tissue and plant regeneration was analyzed by addition of this compound into various cultural media. Preculture of rice calli in the presence of certain levels of 2ip before transferring onto a normal tissue culture regeneration medium increased significantly the rate of embryogenesis as well as plant regeneration. Different combinations of 2ip and 2,4-D in preculture media had profound effects on cell differentiation. The procedures of embryogenesis and plant regeneration can be separated by manipulating the ratio of 2,4-D to 2ip in the culture media. Various possible functions of 2ip, 2,4-D and KT in cereal regeneration are also suggested.Abbreviations: 2ip = isopentenyl adenine; 2,4D = 2,4-dichlorophenoxyacetic acid; KT = kinetin; IAA = indole acetic acid

Re-evaluation of the effects of growth regulators on callus induction and shoot regeneration in Agrobacterium-mediated transformation of lettuce

“…In addition, new growth regulator combinations that included 2ip were tested for this study. The 2ip has been known to be a highly effective cytokinin source for regeneration of monocots and dicots (Zhu et al 1996;Ganesan and Jayabalan 2006). However, no studies have been reported the role of 2ip as a cytokinin source for regeneration of lettuce.…”

Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati

“…Partial physical desiccation treatments have been reported to be beneficial for embryogenesis and plant regeneration in several plant species (De Gloria et al, 2000;Tyagi et al, 2007;Corredoira et al, 2008;Mingozzi et al, 2009). The dehydration of cell suspension derived from the embryogenic calluses (Tsukahara and Hirosawa, 1992;Jain et al, 1996;Zhu et al, 1996;Chand and Sahrawat, 2001) can increase plant regeneration efficiency. On the basis of this idea, we aimed to observe the effect of chemical desiccation through maltose and sorbitol supplements (to increase osmotic pressure) in place of sucrose in the plant regeneration medium (PRM), with an additional dehydration desiccation treatment on proliferating calluses prior to their regeneration.…”

The Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) Genotypes

“…2,4-D is one of the important synthetic auxins which is extensively used in tissue culture for callus induction (Zhu et al, 1996;Sankepally and Singh, 2016). Many reports suggest that media supplemented 2,4-D along with BA (Sahoo et al, 2011) or NAA (Bano et al, 2005) give better callus induction rate.…”