The Effectiveness of Suffruticosol B in Treating Lung Cancer by the Laser Trapping Technique

Abstract

We used laser trapping to study the effects of suffruticosol B on lung cancer cells. Physical and mechanical changes were found to be statistically significant, with a 63.97% increase over untreated cells and a 79.57% increase over untreated cells after treatment for 3 or 6 h, respectively. The treatment affected the internal structure of the cells, with changes in their elastic properties. The cellular responses showed that treatment with suffruticosol B resulted in the decreased proliferation and invasion of cancer cells. These results suggest that the treatment may be useful in preventing or treating lung cancer.

Comparative Analysis of Radiation Response in Chemo-Treated BT20, 4T1 Breast Cancer, and Neuroblastoma Cancer Cell Lines through Single and Multiple Cell Ionization Using Infrared Laser Trapping

“…1 illustrates a schematic of the laser trapping set-up. This experimental setup replicates the recently reported studies associated with single-cell trapping for cell deformation or cell ionization [22][23][24][25][26][27][28][29]. Here we brie y describe the functions of the basic elements in the setup.…”

“…We assessed the radiosensitivity of BT20-treated cells and compared it to that of 4T1 and N2a cancer cell lines. To calculate the TIE and TRD, we used the laser trapping technique developed by Erenso and colleagues [22][23][24][25][26][27][28][29]. We demonstrated here, that the antitumor compound DMDD increased the radio-sensitivity of BT20, with the most signi cant difference being that the TRD decreased with increased cell mass.…”

“…The electrons are pulled apart from the atom to ionize the cell permanently. As a result, the gradual intense charge build-up leads to an electrostatic force that exceeds the intensity gradient trap force [22][23][24][25][26][27][28][29], and at this instant, the cell gets ejected from the trap.…”

“…Finding the TRD was computed for each of the BT20 cells using TIE is the minimum radiation energy the cell needs to experience irreversible damage as the cell becomes a signi cant charge by ionization. When the cell reaches this threshold charge, the stronger electrostatic force overcomes the trapping force causing the cell to eject from the trap [22][23][24][25][26][27][28][29].…”

“…4(d, e) shows that for the TRD. The statistical reduction method from our studies [22][23][24][25][26][27][28][29] was followed by sorting each data by TIE or TRD in ascending order. Thus, the reduced data obtained is shown in Fig.…”