The Effectiveness of Poly-(4-vinyl-N-hexylpyridiniumbromide) as an Antibacterial Implant Coating: An<i>In Vitro</i>Study

Ringenberg, L.; Winkel, Andreas; Kufelt, Olga; Behrens, Peter; Stiesch, Meike; Heuer, Wieland

doi:10.1155/2011/859140

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…In this study, coating with VP:DMMEP 30:70 led to a 90% decrease in the bacterial load of S. aureus and S. epidermidis after 5 h of incubation in comparison to uncoated samples. This confirmed results from a previous study with only 1 h incubation [ 14 ]. Similar or even better results could be achieved for S. sanguinis , a primary colonizer of human teeth and dental implants.…”

Section: Resultssupporting

confidence: 92%

Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces

Winkel

Dempwolf

Gellermann

et al. 2015

IJMS

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Peri-implant infections from bacterial biofilms on artificial surfaces are a common threat to all medical implants. They are a handicap for the patient and can lead to implant failure or even life-threatening complications. New implant surfaces have to be developed to reduce biofilm formation and to improve the long-term prognosis of medical implants. The aim of this study was (1) to develop a new method to test the antibacterial efficacy of implant surfaces by direct surface contact and (2) to elucidate whether an innovative antimicrobial copolymer coating of 4-vinyl-N-hexylpyridinium bromide and dimethyl(2-methacryloyloxyethyl) phosphonate (VP:DMMEP 30:70) on titanium is able to reduce the attachment of bacteria prevalent in peri-implant infections. With a new in vitro model with semi-coated titanium discs, we were able to show a dramatic reduction in the adhesion of various pathogenic bacteria (Streptococcus sanguinis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis), completely independently of effects caused by soluble materials. In contrast, soft tissue cells (human gingival or dermis fibroblasts) were less affected by the same coating, despite a moderate reduction in initial adhesion of gingival fibroblasts. These data confirm the hypothesis that VP:DMMEP 30:70 is a promising antibacterial copolymer that may be of use in several clinical applications.

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Section: Resultssupporting

confidence: 92%

Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces

Winkel

Dempwolf

Gellermann

et al. 2015

IJMS

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“…In contrast, for initial adhesion experiments, bacteria were suspended in buffer solution without nutrients, and samples were incubated under agitation for 5 h only. This leads to the formation of a homogeneous single layer of adhering bacterial cells on the surface, whereas biofilm outgrowth is inhibited. − The number of adhering bacteria and the live/dead distribution was determined for each spike/lubricant combination. As depicted in Figure C, only the lubricants 143 AZ, GPL 104, and GPL 105 gave a statistically significant reduction in adhering cells of ∼10-fold compared to that of the unstructured, uncoated control.…”

Section: Resultsmentioning

confidence: 99%

Development of Laser-Structured Liquid-Infused Titanium with Strong Biofilm-Repellent Properties

Döll

Fadeeva

Schaeske

et al. 2017

ACS Appl. Mater. Interfaces

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Medical implants are commonly used in modern medicine but still harbor the risk of microbial infections caused by bacterial biofilms. As their retrospective treatment is difficult, there is a need for biomedical materials that inhibit bacterial colonization from the start without using antibacterial agents, as these can promote resistance development. The promising concept of slippery liquid-infused porous surfaces (SLIPS) possesses enormous potential for this purpose. In the present study, this principle was applied to titanium, a common material in implantology, and its biofilm-repellent properties were demonstrated. To simplify prospective approval of the medical device and to avoid chemical contamination, surface structuring was performed by ultrashort pulsed laser ablation. Four different structures (hierarchical micro- and nanosized spikes, microsized grooves, nanosized ripples, and unstructured surfaces) and five infusing perfluoropolyethers of different viscosities were screened; the best results were obtained with the biomimetic, hierarchical spike structure combined with lubricants of medium viscosities (20-60 cSt at 37 °C, 143 AZ, and GPL 104). The surfaces exhibited extremely low contact angle hysteresis, as is typical for liquid-infused materials and a reliable 100-fold reduction of human oral pathogen Streptococcus oralis biofilms. This characteristic was maintained after exposure to shear forces and gravity. The titanium SLIPS also inhibited adherence of human fibroblasts and osteoblasts. Toxicity tests supported the explanation that solely the surface's repellent properties are responsible for the vigorous prevention of the adhesion of bacteria and cells. This use of physically structured and liquid-infused titanium to avoid bioadhesion should support the prevention of bacterial implant-associated infections without the use of antibacterial agents.

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“…Although not cytotoxic for human gingival fibroblasts, compounds containing quaternary ammonium, phosphonium, or pyridinium ions, like poly-(4-vinyl-N-hexylpyridinium bromide) appear to be weaker disinfectants and implant coatings have only moderate antimicrobial activity for oral microorganisms like Streptococcus mutans or Streptococcus sanguinis [44].…”

Section: Antiseptics and Disinfectants In Bioactive Coatingsmentioning

confidence: 99%

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

et al. 2020

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Replacement of missing teeth is an essential component of comprehensive dental care for patients suffering of edentulism. A popular option is implant-supported restorations. However, implant surfaces can become colonized with polymicrobial biofilms containing Candida species that may compromise peri-implant health. To prevent this, implant components may be treated with a variety of coatings to create surfaces that either repel the attachment of viable microorganisms or kill microorganisms on contact. These coatings may consist of nanoparticles of pure elements (more commonly silver, copper, and zinc), sanitizing agents and disinfectants (quaternary ammonium ions and chlorhexidine), antibiotics (cefalotin, vancomycin, and gentamicin), or antimicrobial peptides (AMPs). AMPs in bioactive coatings have a number of advantages. They elicit a protective action against pathogens, inhibit the formation of biofilms, are less toxic to host tissues, and do not prompt inflammatory responses. Furthermore, many of these coatings may involve unique delivery systems to direct their antimicrobial capacity against pathogens, but not commensals. Coatings may also contain multiple antimicrobial substances to widen antimicrobial activity across multiple microbial species. Here, we compiled relevant information about a variety of creative approaches used to generate antimicrobial prosthetic surfaces in the oral cavity with the purpose of facilitating implant integration and peri-implant tissue health.

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The Effectiveness of Poly-(4-vinyl-N-hexylpyridiniumbromide) as an Antibacterial Implant Coating: AnIn VitroStudy

Cited by 6 publications

References 29 publications

Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces

Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces

Development of Laser-Structured Liquid-Infused Titanium with Strong Biofilm-Repellent Properties

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

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