The effectiveness of passive ultrasonic irrigation on intraradicular <i>Enterococcus faecalis</i> biofilms in extracted single‐rooted human teeth

Bhuva, Bhavin; Patel, Shanon; Wilson, Ron; Niazi, Sadia; Beighton, David; Mannocci, Francesco

doi:10.1111/j.1365-2591.2009.01672.x

Cited by 85 publications

(106 citation statements)

References 47 publications

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“…Propolis has been studied due several properties, as antimicrobial, anti-inflammatory, antioxidant, and others [11][12][13][14][15][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The chemical compounds found in propolis showed antimicrobial and tissue tolerance characteristics.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial action of red and green propolis extract in infected root canal

Endo

Estrela

Alencar

et al. 2017

Rev. Odonto Ciênc. (Online)

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OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals. METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols -passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium. RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective. CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti-inflammatory, antioxidant and low toxicity properties. Key words: Endodontic; Propolis; Sodium hypochlorite Ação antimicrobiana do extrato de própolis vermelho e verde em canais radiculares infectados RESUMO OBJETIVO: Investigar a ação antibacteriana do própolis vermelhos 30%, do própolis verde 40% e do hipoclorito de sódio 2,5% com protocolos de irrigação em canais radiculares infectados. MÉTODOS: Durante 60 dias, vinte e quatro canais radiculares foram inoculados com E. faecalis. Em todos os grupos experimentais foi realizado o preparo do canal radicular e dois protocolos de irrigação -irrigação ultra-sônica passiva e irrigação convencional. Nos grupos 1, 3, 5 e 7 foi feito o preparo do canal radicular associado à irrigação convencional com própolis 30%, própolis 40%, hipoclorito de sódio 2,5% e água destilada, respectivamente. Nos grupos 2, 4, 6 e 8 foi feito o preparo do canal radicular associado à irrigação ultra-sônica passiva com as soluções descritas acima. Os grupos 9 e 10 foram os controles (negativos e positivos). Amostras dos canais radiculares foram coletadas e imersas em 7 mL de BHI, durante 48 horas, a 37ºC. O crescimento bacteriano foi analisado por turvação do meio de cultura. RESULTADOS: Ação antibacteriana foi observada com própolis vermelho 30% e hipoclorito de sódio 2,5% quando utilizado irrigação ultra-sônica passiva e irrigação convencional após 20 minutos, porém, após 72 horas as substâncias não foram efetivas. CONCLUSÃO: Os agentes de irrigação e os protocolos não foram efetivos para eliminar os microorganismos dos canais radiculares infectados.

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Section: Discussionmentioning

confidence: 99%

“…In this regard, propolis has been investigated for presenting antimicrobial properties, anti-inflammatory, antioxidant, and others [11][12][13][14][15][16][17][18][19][20][21][22][23][24]. Because of its low toxicity, it has been studied as possible intracanal medication [11,12].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial action of red and green propolis extract in infected root canal

Endo

Estrela

Alencar

et al. 2017

Rev. Odonto Ciênc. (Online)

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“…Biofilm found in vivo in teeth with apical periodontitis is mature, strongly adhered to the substrate and penetrating into dentinal tubules, which make it more resistant to cleaning and shaping [14]. To obtain consistent and organized biofilm samples, E. faecalis was incubated for 60 days.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Zechin

Steier²,

Rossi‐Fedele

et al. 2016

Rev. Odonto Ciênc. (Online)

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Objective: To evaluate the use of optic fiber and different pre-irradiation times (PIT) in phototherapy with LED for the root canal disinfection of bovine teeth infected in vitro with Enterococcus faecalis. Methodology: The teeth were incubated for 60 days and divided into six groups according to disinfection protocol: Group 1 -distilled water; Group 2 -1 minute PIT and no optic fiber; Group 3 -1 minute PIT and optic fiber; Group 4 -5 minute PIT and no optic fiber; Group 5 -5 minute PIT and optic fiber; Group 6 -2% sodium hypochlorite. Microbiological test (CFUs counting) and scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments. Results: SEM analysis of canal wall, in the three thirds, revealed group 6 had the best results, whereas microbiological results revealed that group 6 had the best results. No differences in disinfection of root canals were found when optic fiber was used for photodynamic therapy. Conclusion: Photodynamic therapy should not be used alone in the disinfection of root canals, but it may be valuable as a complement for disinfection performed using different cleaning methods. Análise comparativa de redução microbial usando terapia fotodinâmica em dentes bovinos infectados com Enterococcus faecalis -um estudo in vitro RESUMO Objetivo: Avaliar, in vitro, o uso da fibra óptica e diferentes tempos de pré-irradiação (PIT) na terapia fotodinâmica para a descontaminação do canal radicular de dentes bovinos infectados com Enterococcus faecalis. Metodologia: Os dentes foram incubados por 60 dias e divididos em 6 grupos de acordo com o protocolo de descontaminação: Grupo 1 -água destilada; Grupo 2 -1 minuto de pré-irradiação sem fibra óptica; Grupo 3 -1 minuto de pré irradiação com fibra óptica; Grupo 4 -5 minutos de pré-irradiação sem fibra óptica; Grupo 5 -5 minutos de pré-irradiação com fibra óptica; Grupo 6 -hipoclorito de sódio 2%. Teste microbiológico (contagem de UFCs) e microscopia eletrônica de varredura (MEV) foram realizados para avaliar a eficácia dos tratamentos propostos. Resultados: A análise em microscopia electronica de varredura das paredes do canal radicular, nos três terçoes, bem como a análise microbiológica, revelou que o grupo 6 obteve o melhor resultado no processo de descontaminação. Não foram encontradas diferenças estatisticamente significantes na desinfecção do canal radicular comparando o uso ou não da fibra óptica. Conclusão: Terapia fotodinâmica isoladamente não deveria ser utilizada na desinfecção do canal radicular, mas pode ser uma alternative viável como complemento a partir da utilização de diferentes métodos de limpeza.

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“…Specimens were placed in a small flask containing silicone-based impression material (Zetaplus, Zhermack, Italy) and embedded up to 1 mm from the coronal surface of the sectioned root in order to simulate the periodontal ligament, in accordance with Wilcox et al [25] and Bhuva et al [26], during instrumentation and obturation of the root canal.…”

Section: Matrix Constructionmentioning

confidence: 99%

Incomplete vertical root fracture associated with lateral compaction technique: a microscopic analysis

Chávez-Andrade¹

2013

BDS

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Objective:This study evaluated the effects of root canal obturation employing lateral compaction technique and spreader load of 1.5 kg on the incidence of complete (CVRF) or incomplete vertical root fractures (IVRF). Material and Methods: Twenty-seven distal roots of extracted human mandibular molars were used. All root canals were prepared by biomechanical step-back technique and obturated by lateral compaction technique. The prepared roots were distributed into two groups: G1- experimental (n=17) and G2- control (n=10). During obturation, load of 1.5 kg was applied to a size 30 finger spreader. Pre- and post-obturation images of the coronal portion of the roots were captured by inverted digital microscopy and analyzed by one trained examiner. Data were evaluated by Fisher’s test (p < 0.05) using GrapH Pad Prism 5.0. Results: No roots exhibited CVRF. All fractures observed before and after obturation were IVRF or “other defects”. In G2 (control group), there was no increase of IVRF number. Interestingly, G1 presented an increase in the IVRF number to 70,59% in the 12 teeth out of 17 teeth studied. The statistical analysis showed that the mean of IVRF increased significantly in G1 when compared to G2 (p < 0.05). Conclusions: The application of a 1.5 kg spreader load during lateral compaction technique does not produce complete vertical root fractures, but may produce incomplete fractures or “other defects”.

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The effectiveness of passive ultrasonic irrigation on intraradicular Enterococcus faecalis biofilms in extracted single‐rooted human teeth

Abstract: Both conventional syringe irrigation and passive ultrasonic irrigation with 1% sodium hypochlorite were effective at completely removing intraradicular E. faecalis biofilms. Conventional syringe irrigation with sterile saline solution was only partially effective at removing the biofilms.

Cited by 85 publications

References 47 publications

Antibacterial action of red and green propolis extract in infected root canal

Antibacterial action of red and green propolis extract in infected root canal

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Incomplete vertical root fracture associated with lateral compaction technique: a microscopic analysis

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