The effectiveness of calcium hydroxide paste mixed with 2% chlorhexidine solution against Candida albicans-infected human roots: An ex vivo study

Alsalleeh, Fahd; Almohaimeed, Amani; Almoqbel, Anfal; Allahem, Ziyad

doi:10.4103/endo.endo_127_18

Cited by 1 publication

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“…It has been shown that C. albicans were resistant to Ca(OH) 2 and additional medications were required to increase the effectiveness of anti-fungal activity [ 25 ]. It was reported that mixing Ca(OH) 2 with chlorhexidine was more effective in eliminating C. albicans [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans: An Ex Vivo Analysis

Alghofaily,

Alfraih,

Alsaud

et al. 2024

Microorganisms

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Introduction: The purpose of this study was to assess the antifungal activity of silver nanoparticles (AgNPs) in combination with calcium hydroxide (Ca(OH)2) against Candida albicans (C. albicans). Methods: AgNPs was mixed with pure Ca(OH)2 powder in an aqueous base. A standard suspension (1 × 108 bacterial cells/mL) of C. albicans was prepared in a 96-well plate and incubated on shaker at 37 °C in 100% humidity to allow fungal biofilm formation in infected dentin slices (n = 98). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AgNPs alone or with Ca(OH)2 were determined. The samples were separately placed in 24-well tissue culture plates and divided into three experimental groups (0.03, 0.04, and 0.06) and three control groups; negative (saline) and positive chlorhexidine gel and Ca(OH)2. Quantitative measurements of fungal activity by XTT colorimetric assay and qualitative measurements using confocal laser microscopy and scanning electron microscopy were performed. Results: The cell viability of C. albicans in the experimental groups was significantly reduced compared to the negative control group. The combination of (AgNPs (0.04%) and Ca(OH)2) was the most potent against C. albicans. Conclusions: The findings demonstrated that combining silver nanoparticles with Ca(OH)2 was more effective against C. albicans biofilm compared to Ca(OH)2 alone, suggesting a combing effect.

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Section: Introductionmentioning

confidence: 99%