THE EFFECT OF ω-FATTY ACIDS ON mRNA EXPRESSION LEVEL OF PPARγ IN PATIENTS WITH GASTRIC ADENOCARCINOMA

Abstract

Background: The antineoplastic role of peroxisome proliferator-activated receptor gamma (PPARγ) ligandshas previously been demonstrated in several gastric cancer cell lines. Activation of PPARγ by polyunsaturated fatty acids (PUFAs) inhibits growth and proliferationof tumor cells. In this double-blind clinical study, we evaluate the effect of PUFAs on PPARγ mRNA expression in patients with gastric adenocarcinoma. Materials and Methods: A total of 34 chemotherapy-naive patients diagnosed with gastric adenocarcinoma were enrolled in the present study. According to treatment strategies, all subjects were divided into two groups, the first group (17 individuals) received cisplatin without supplements and the second group (17 individuals) received cisplatin plus orally administered PUFAs supplements for 3 weeks. The gastric biopsy samples were obtained from all participants before and after treatment, and PPARγ mRNA expression levels were evaluated by quantitative real-time polymerase chain reaction using validated reference genes. Results: Our findings revealed that PPARγ mRNA expression is significantly upregulated in group II afterreceiving cisplatin plus orally administered PUFAs supplements for three weeks (p < 0.0001), whereas PPARγ mRNA expression did not show significant alteration in group I after receiving cisplatin alone. Conclusion: The results of the study evidence that PPARγ may act as a potential target for the therapy of human gastric adenocarcinoma.

PHTF2 regulates lipids metabolism in gastric cancer

“…In order to better understand the functions of DEGs in gastric cancer cells, GO and KEGG pathway enrichment analysis revealed that DEGs were mainly involved in intracellular signal transduction, energy metabolism, and fatty acid metabolism. This was consistent with the idea that the pathological process of gastric cancer was usually accompany with abnormalities in fatty acid metabolism [9,25]. Through computing the relevance of the constructed PPI network, 20 highly central genes were found to be the key nodes.…”

“…Interestingly, studies have revealed that more lipid metabolism-related proteins, identified as oncogenes in eukaryotic cells, were highly expressed in many human cancers [6,7]. A high prevalence of gastric cancer was found in overweight individuals by epidemiological studies, and the significance of fatty acid metabolism in the development and progression of GC was increasingly recognized by scholars [8,9]. Moreover, lipids accumulation was also observed in the pathological tissues in GC [10].…”

Lipid Metabolic-Related Signature CYP19A1 is a Potential Biomarker for Prognosis and Immune Cell Infiltration in Gastric Cancer

“…This was consistent with the previous research that lipogenesis is important for various tumor cell growth. 35,36 The above data implied the molecular mechanism underlying lipid metabolism in GC.…”

Marcadores inflamatórios em pacientes com neoplasia gástrica: níveis sanguíneos e expressão gênica

“…O aumento da expressão do RNAm de TNF- em pacientes com neoplasia gástrica foi associado à presença do vírus H. pilory (Zabaglia et al, 2017;Hosseinzadeh et al, 2016 (Liu et al, 2008). Não houve diferença na expressão de RNAm de TNF-α em tecido neoplásico gástrico antes e após 3 semanas do emprego do quimioterápico cisplatina (Hosseinzadeh et al, 2016), assim como entre indivíduos que receberam ou não quimioterapia neoadjuvante para câncer gastroesofágico (Deans et al, 2006). O emprego das drogas 5-fluoracil e cisplatina associadas à radioterapia resultou em maior expressão de RNAm de TNF-α no sangue total de pacientes com tumor de esôfago (Bowen et al, 2015).…”

“…Em estudo in vitro e in vivo demonstrou que a expressão do RNAm do PPAR-γ no tecido tumoral gástrico está associado com doença maligna em estágio menos avançado e com uma melhor sobrevida, sugerindo o uso do PPARγ como marcador de menor agressividade do carcinoma gástrico . Na literatura, a expressão de RNAm do PPAR-γ em tecido tumoral gástrico foi semelhante quando avaliado antes e após quimioterapia com cisplatina (Hosseinzadeh et al, 2016). No presente estudo, a expressão de RNAm da ZAG em sangue total foi semelhante entre todos os grupos de estudo, independente de apresentarem ou não neoplasia gástrica.…”