The effect of γ-radiation on induction of the hobo element transposition in Drosophila melanogaster

Zakharenko, L. P.; Kovalenko, L. V.; Perepelkina, M. P.; Захаров, И. К.

doi:10.1134/s1022795406060056

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…For example, X-radiation was associated to P element transposition activation (Eeken and Sobels, 1986;Margulies and Griffith, 1991). However, g-radiation induces transposition of 412 retrotransposon (Zabanov et al, 1995), but not of hobo (Zakharenko et al, 2006) in D. melanogaster. It is known that radiations can induce double-strand DNA breakage, leading to a reorganization of the genetic material.…”

Section: Abiotic Stressesmentioning

confidence: 99%

“…It is known that radiations can induce double-strand DNA breakage, leading to a reorganization of the genetic material. On the other hand, it was suggested that ionizing effects of radiations were associated to increases of recombination frequencies (Zakharenko et al, 2006). TE insertion location can be affected by these phenomena, and new positions detected could result from the chromosomal changes and not from new transpositions.…”

Section: Abiotic Stressesmentioning

confidence: 99%

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What makes transposable elements move in the Drosophila genome?

Guerreiro

2011

Heredity

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Transposable elements (TEs), by their capacity of moving and inducing mutations in the genome, are considered important drivers of species evolution. The successful invasions of TEs in genomes, despite their mutational properties, are an apparent paradox. TEs' transposition is usually strongly regulated to low value, but in some cases these elements can also show high transposition rates, which has been associated sometimes to changes in environmental conditions. It is evident that factors susceptible to induce transpositions in natural populations contribute to TE perpetuation. Different factors were proposed as causative agents of TE mobilization in a wide range of organisms: biotic and abiotic stresses, inter-and intraspecific crosses and populational factors. However, there is no clear evidence of the factors capable of inducing TE mobilization in Drosophila, and data on laboratory stocks show contradictory results. The aim of this review is to have an update critical revision about mechanisms promoting transposition of TEs in Drosophila, and to provide to the readers a global vision of the dynamics of these genomic elements in the Drosophila genome.

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Section: Abiotic Stressesmentioning

confidence: 99%

Section: Abiotic Stressesmentioning

confidence: 99%

What makes transposable elements move in the Drosophila genome?

Guerreiro

2011

Heredity

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“…The strains were obtained from the Bloomington Drosophila Stock Center and characterized using molecular (PCR analysis) and genetic (instability of the mini-white locus) methods, which made it possible to determine the content of full-length hobo copies in their genomes (Galindo et al, 1995;Bazin and Higuet, 1996;Zakharenko et al, 2006;Yushkova and Zainullin, 2014).…”

Section: Fly Strainsmentioning

confidence: 99%

“…Recently, there has been a growing interest in studies of the behavior of hobo elements which not only can be massively activated under conditions of classical dysgenic crossing [Hmales (with hobo transposons) × E-females (without hobo)] (Blackman et al, 1987) but they can also be active in nongenetic systems, i.e., under conditions of interlinear (between H-males and H-females) and reciprocal (between H-females and E-males) crosses (Bazin and Higuet, 1996;O'Hare et al, 1998;Zakharenko et al, 2006;Yushkova, 2019). The hobo transposon of D. melanogaster belongs to the Ac elements of the hAT superfamily (Loreto et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The hobo transposon of D. melanogaster belongs to the Ac elements of the hAT superfamily (Loreto et al, 2018). It is active in the germ line and somatic tissue cells (Calvi and Gelbart, 1994;O'Hare et al, 1998;Zakharenko et al, 2006) and also is sensitive to radiation exposure (Ivashenko and Grishaeva, 2002;Yushkova and Zainullin, 2014). The full-length hobo element (∼ 3 kb) has an open reading frame (ORF) encoding transposase and short terminal inverted repeats (TIRs) 12 bp in size, forms duplications at the insertion site of 8 bp (Loreto et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Yushkova

2020

Front. Genet.

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The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.

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