The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza

Chen, Hui; Chena, Feng; Chiu, F.C.K.; Lo, Cindy M. Y.

doi:10.1016/s0141-0229(00)00284-2

Cited by 115 publications

(58 citation statements)

References 20 publications

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“…The increase in intracellular colchicine content may be attributed to the fact that jasmonates are signal compounds in the elicitation process leading ultimately to the biosynthesis of secondary metabolites in plant cell cultures (Furmanowa et al 1997). This result was similar to those observed in several elicitation experiments where growth was inversely related to secondary metabolite accumulation in cell and organ cultures (Furmanowa et al 1997, Chen et al 2001.…”

Section: Effect Of Biotic Elicitors On Growth and Colchicine Content supporting

confidence: 90%

“…The application of elicitors to plant cell and organ cultures is useful for enhancing the biotechnological productivity of valuable secondary metabolites in vitro (Buitelaar et al 1992, Vazquez et al 1994, Chen et al 2001). An added biotechnological benefit is that they also promote release of the metabolites into the medium (PittaAlvarez et al 2000a).…”

Section: Introductionmentioning

confidence: 99%

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Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Ghosh

Ghosh²,

Jha

2006

Biotechnol Lett

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Root cultures of Gloriosa superba were treated with 5 mM: methyl jasmonate and 125 microM: AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mM: CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mM: CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.

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Section: Effect Of Biotic Elicitors On Growth and Colchicine Content supporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Ghosh

Ghosh²,

Jha

2006

Biotechnol Lett

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“…The hairy roots were harvested for analyses at 0, 12, 24, 36 and 48 h after elicitation. Dry weight determination was carried out as previously described (Chen et al 2001).…”

Section: Elicitation Of Tomato Hairy Root Culturesmentioning

confidence: 99%

Accumulation of cell wall-bound phenolic metabolites and their upliftment in hairy root cultures of tomato (Lycopersicon esculentum Mill.)

Mandal

Mitra

2008

Biotechnol Lett

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Alkaline hydrolysis of cell wall material of tomato hairy roots yielded ferulic acid as the major phenolic compound. Other phenolics were 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin and 4-coumaric acid. The content of phenolics was much higher at the early stage of hairy root growth. The ferulic acid content decreased up to 30 days and then sharply increased to 360 microg/g at 60 days of growth. Elicitation of hairy root cultures with Fusarium mat extract (FME) increased ferulic acid content 4-fold after 24 h. As the pathogen-derived elicitors have specific receptors in plants, FME may thus be used for inducing resistance against Fusarium oxysporum f. sp. lycopersici.

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“…Methyl jasmonate (MeJA) is a signal molecule applied for enhancing the production of secondary metabolites in several hairy root cultures (Aftab et al 2011;Shukla et al 2010). Yeast extract elicitor (YE) is mainly composed of polysaccharide obtained by steps of water extraction and alcohol precipitation, increases both intracellular and extracellular CT content, and improves the growth of S. miltiorrhiza hairy root (Chen et al 2001). It is affirmed that both tanshinone content and related enzyme activity are enhanced by Ag + and YE in hairy root cultures of S. miltiorrhiza, respectively (Ge and Wu 2005).…”

Section: Introductionmentioning

confidence: 99%

Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

Wang

et al. 2015

Protoplasma

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Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes in the tanshinone biosynthetic pathway were remarkably upregulated with elicitation. Yeast extract elicitor heightened 13.9-fold of isopentenyl diphosphate isomerase expression level at 12 h, while it increased 16.7-fold of geranylgeranyl diphosphate synthase transcript at 24 h compared with that of the control, which was more effective than Ag(+) and methyl jasmonate. This study provided a convenient hairy root culture system of S. castanea Diels f. tomentosa Stib. for tanshinone production for the first time.

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The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza

Cited by 115 publications

References 20 publications

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Accumulation of cell wall-bound phenolic metabolites and their upliftment in hairy root cultures of tomato (Lycopersicon esculentum Mill.)

Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

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