The effect of X-rays and ultraviolet light on the uptake in vitro of [8-14C]adenine and [2-14C]-phenylalanine by isolated nuclei

The effects of x-irradiation on the incorporation of amino acids into proteins of nuclei from rat thymus gland

“…The results are given as means+ S.D., with the numbers of estimations in parentheses. 100-0 0-6+0-6 (3) 0-4±0-2 (2) 8-1±0-4 (3) 7-5+1-1 (2) 30-0±6-1 (2) 2-1±1-2 (2) 91-0±3-0 (4) 111-0±6-0 (4) 100- 5+1-5 (2) 113-0±8-0 (4) 95-0±2-0 (3) Errera & Ficq, 1959;Faures & Errera, 1962) in their radioautographic studies. The similarity of our observations indicates that comparable results are obtained by grain counting of radioautographic films and by direct assay of the incorporated radioactivity in a counter.…”

Phosphate-transfer reactions by nuclei from rat thymus gland

“…The reaction was stopped by the addition of perchloric acid and the inorganic phosphate (Pi) and acid-labile phosphate (P15) were determined on the deproteinized extract. Nuclear enzymes and substrates Insight into the mechanism whereby ATP disappeared from nuclei having an (acid-labile phosphate): (inorganic phosphate) ratio > 2 came from the observation that nuclei isolated in sucrose-calcium chloride solution did not show the reaction but would do so if supplemented with sodium chloride, magnesium chloride and glucose (Logan, Errera & Ficq, 1959). This suggested that a Mg2+ ion-dependent kinase might be involved.…”