The effect of weak-inertia on droplet formation phenomena in T-junction microchannel

Azarmanesh, Milad; Farhadi, Mousa

doi:10.1007/s11012-015-0245-6

Cited by 21 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The validation of the simulation results is disseminated into the dripping instability at T-junction and Rayleigh-Plateau instability of the Droplet phase. The dripping instability at the T-junction for the formation of the Sheath is validated against the experimental data of Yeom & Lee 50 and reported in our previous work 9 . The Rayleigh-Plateau instability of the Droplet phase arising at the expansion section is validated against the Gerris code 45 .…”

Section: Methodssupporting

confidence: 69%

“…The injection process will not be successful if the flow patterns of the pulsating flows are not regulated properly. The value π × 555 is set for γ after selecting the velocities of the main channel and the T-junction based on the needs of dripping instability 9 . Thus, it is crucial to precisely measure the period of droplet formation because the performance is highly dependent on the exact timing.…”

Section: Resultsmentioning

confidence: 99%

“…Encapsulation of drug molecules and species like viruses inside a single cell, or delivery of molecules and cells into droplets has led to the creation of compound structures 1–4 , controlled chemical reactions 1,5 , development of drugs for evolving cells and enzymes 6 , and regulation of cell fates. The delivery of particles into droplets have been established using double emulsion techniques such as hierarchical T-junction 7–11 , flow-focusing 2,8,9,12,13 , co-flowing 14,15 , K-channel 16 and cross-flowing 4,17 . Droplet microfluidics is used for capturing and indexing thousands of individual cells in nano-liter droplets 18 , suitable for small in vivo clinical samples like tumors and tissue micro biopsies 19–21 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells

Azarmanesh

Dejam

Azizian

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Microinjection is an effective actuation technique used for precise delivery of molecules and cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into single cells. Several microinjection techniques have been developed for actuating droplets and cells. However, they are still time-consuming, have shown limited success, and are not compatible with the needs of high-throughput (HT) serial microinjection. We present a new passive microinjection technique relying on pressure-driven fluid flow and pulsative flow patterns within an HT droplet microfluidic system to produce serial droplets and manage rapid and highly controlled microinjection into droplets. A microneedle is secured within the injection station to confine droplets during the microinjection. The confinement of droplets on the injection station prevents their movement or deformation during the injection process. Three-dimensional (3D) computational analysis is developed and validated to model the dynamics of multiphase flows during the emulsion generation. We investigate the influence of pulsative flows, microneedle parameters and synchronization on the efficacy of microinjection. Finally, the feasibility of implementing our microinjection model is examined experimentally. This technique can be used for tissue engineering, cells actuation and drug discovery as well as developing new strategies for drug delivery.

show abstract

Section: Methodssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells

Azarmanesh

Dejam

Azizian

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The dynamics of droplet formation in a second fluid inside microchannels have important applications in drug delivery (Richter et al, 1997), food industries (Cal, 2005), mixing in microfluidic multiphase systems (Losey et al, 2002), and a wide range of applications in biology and chemistry. Monodisperse droplets can be generated via different methods in microfluidic devices, including cross-junction devices Brazilian Journal of Chemical Engineering (Yasuno et al, 2004;Sugiura et al, 2004;Elzanfaly et al, 2015) , T-junctions (Cheng et al, 2016;Liu et al, 2015;Sbragaglia et al, 2016;Thorsen et al, 2001;Nisisako et al, 2002;Xu et al, 2006;Garstecki et al, 2006;Van der Graaf et al, 2006;Christopher et al, 2008;Azarmanesh and Farhadi, 2015;Malekzadeh and Roohi, 2015), flow-focusing devices (Anna et al, 2003;Cubaud et al, 2005;Garstecki et al, 2005;Fu et al, 2009) and co-flowing devices (Umbanhowar et al, 2000;Hua et al, 2007;Alizadeh et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Different Stages of Liquid Film Growth in a Microchannel: Two-Phase Lattice Boltzmann Study

Nazari

Sani

Kayhani

et al. 2018

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

A free energy model is used to describe the droplet formation and break-up process in a T-junction bio-microchannel. Droplets are created as a result of interaction of two immiscible liquids. Different stages for the droplet formation process are analyzed which are: a) growing in the x and y directions, b) growing in the x direction and c) detachment process. The effects of capillary number and flow rate ratio on the droplet formation stages are also studied. The influences of Capillary number, flow rate ratio, viscosity ratio and geometrical parameters on droplet break up, droplet size and detachment time are systematically studied. By increasing the flow rate ratio; the duration of droplet formation and the length of the x-growth stage are decreased for small capillary numbers. For larger capillary numbers; the droplet penetrates toward the downstream; therefore, the length of the x-growth stage is increased. The start of detachment process in the microchannel is also reported, which is related to narrowing of the neck of the liquid film. The results show that the detachment time is increased by decreasing the Capillary number. For Ca>0.02, the detachment time is independent of the flow rate ratios. Moreover; the effects of viscosity ratios on detachment time are not significant in comparison to the effects of capillary number. For Ca<0.04, the size of the droplet is independent of the viscosity ratio, but after the critical Capillary number (i.e., Ca=0.04), the size of the droplet is varied by the viscosity ratio. The time between two consecutive drops is also decreased by increasing the Capillary number. Moreover, this time is decreased by increasing the flow rate ratio until Ca=0.04. After this Capillary number, the flow rate ratios have no significant effect on the time between two consecutive droplets. An exhaustive validation study is performed including (a) the Laplace equation in the stationary droplet; (b) a contact angle test; (c) Taylor deformation test in shear flow and (d) comparison of the droplet length as a function of flow rate ratio between the present work and other studies.

show abstract

“…This behavior is due to the interface instability caused by the exerted shear force of air on the water phase. 65 In other cases ( Fig. 5b and d), the water stream extends to the outlet without droplet generation and breakup may occur either close to or aer the outlet (parallel ow regime).…”

Section: Resultsmentioning

confidence: 94%

Numerical simulation of high inertial liquid-in-gas droplet in a T-junction microchannel

2017

View full text Add to dashboard Cite

show abstract

The effect of weak-inertia on droplet formation phenomena in T-junction microchannel

Cited by 21 publications

References 19 publications

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells

Different Stages of Liquid Film Growth in a Microchannel: Two-Phase Lattice Boltzmann Study

Numerical simulation of high inertial liquid-in-gas droplet in a T-junction microchannel

Contact Info

Product

Resources

About