The Effect of Vitamin D and Its Metabolites on Fracture Repair in Chicks

Abstract

One-day-old chicks were depleted of vitamin D. At 3 weeks their right tibiae, and those of a control group given vitamin D3, were fractured and pinned. After fracture the controls were kept on vitamin D3. Another group was left vitamin D-deficient. The remaining depleted chicks, divided into four groups, were given vitamin D3, 24,25-dihydroxyvitamin D3 [24,25(OH)2D3], 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] or a combination of 24,25(OH)2D3 and 1,25(OH)2D3. The callus obtained after 9 and 14 days was subjected to torsional stress. The callus of chicks given vitamin D continuously showed the greatest resistance, whereas that of vitamin D-deficient chicks showed the smallest resistance. Repletion with either vitamin D3 or its metabolites increased the strength of the callus. Repletion with the combination of 24,25(OH)2D3 and 1,25(OH)2D3 produced the most marked results, in that the callus was even stronger than that of chicks replete with vitamin D3. It is concluded that 24,25(OH)2D3 is essential for bone formation in addition to the known active vitamin D metabolite 1,25(OH)2D3, and the possible clinical implications of these findings are discussed.

The potential effects of cholecalciferol on bone regeneration in dogs

“…Most animal models demonstrated a positive correlation of bone fracture healing and sys-temic supplement of vitamin D although an early report claimed an irrelevant association between vitamin D metabolites and bone formation in rats (Thorngren et al 1983). These pertinent evidence support that vitamin D, including 1,25(OH) 2 D 3 and its metabolites, might promote fracture bone repair and increase the strength of the callus in chicks, rats, and rabbits (Brumbaugh et al 1982;Dekel et al 1983;Omeroglu et al 1997;Delgado-Martinez et al 1998). On the other hand, to our knowledge, this is the first report to mention that taking calcium supplementation and cholecalciferol combination (Vit.D/ Ca) may stimulate early bone healing of alveolar sockets in dogs.…”

Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats

“…The internal fixation allowed the rats to use their legs soon after surgery. The extent of initial trauma and the shape and site of fracture were well controlled, and the type of fixation we used enabled the micromovements needed for good fracture healing [2,22].…”

“…The tibia was then gently broken by light manual bending while the Kirschner wire was held in place in the medullary canal. The incision was closed with interrupted silk sutures [2]. The fifty rats were divided in a randomized block design in a blinded fashion into two equal groups.…”

“…Fracture healing may be modified by several factors such as hormones, vitamins, minerals, local vascularity, weight bearing, protein diet, ultrasound, and electrical stimuli [1][2][3][4][5]. Recently, some attention has been paid to another kind of potential stimulating factor, low-power laser irradiation (LPLI).…”

Vitamin D in Foot and Ankle Fracture Healing

“…31 Parathyroid hormone has been given with success in healing pelvic fractures in humans, with or without calcium and vitamin D supplementation. And finally, Holstein et al 41 have shown that folate and B12 do not affect fracture healing.…”