The Effect of Vitamin C Administration on Interleukin-6 in Male Rats of Wistar Strain Infected with Pseudomomonas Aeruginosa Bacteria

Abstract

Pseudomonas aeruginosa is the main pathogen that causes nosocomial infections in hospitals, and can infect open wounds, burns to necrosis pneumonia. Research shows vitamin C contains antioxidants and antimicrobials. To determine the effect of vitamin C administration on reducing interleukin-6 (IL-6) in male rats of wistar strains infected with Pseudomonas aeruginosa bacteria. This study used an experimental research design with a Post Test Only Control Group Design research design. The subjects of the study amounted to 30 wistar rats which were randomly divided into 5 groups. K(-) group of rats without P. aeruginosa infection. K(+) group of rats infected with P. aeruginosa 108 CFU per head without vitamin C. P1, P2 and P3 in P. aeruginosa infection and given vitamin C at doses of 9 mg, 18 mg and 36 mg/mL/day for 7 days. Day 8 examination of IL-6 levels using the ELISA method. The results of IL-6 levels the One Way Anova test showed significant differences between groups (p = 0.028). The decrease in IL-6 levels in the K3 group infected with Pseudomonas aeruginosa bacteria and vitamin C at a dose of 9 mg/ml/ day experienced insignificant differences with the control group (K1), the K4 group given vitamin C at a dose of 18 mg/ml/day and the K5 group given vitamin C at a dose of 36 mg/ml/day.