The effect of Vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp Litopenaeus vannamei

Chang, Chin‐Chyuan; Yeh, Maw-Sheng; Lin, Hung-Kuei; Cheng, Winton

doi:10.1016/j.fsi.2008.09.004

Cited by 61 publications

(24 citation statements)

References 37 publications

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“…The result showed that Mrcasp mRNA was widely expressed in all examined tissues (Fig. 3), similar to the expression of caspase-3 mRNA of P. vannamei which was found in all examined tissues especially in hemocytes and the lymphoid organ [31]. Lvcaspase2 to 5 transcripts were also found in all tested tissues with variable expression levels [40].…”

Section: Tissue Expression Of Mrcaspsupporting

confidence: 66%

“…The predicted molecular weight and the pI were 35.87 kDa and 5.78, respectively. A putative caspase active site, QACRG pentapeptide, found in several shrimp caspase sequences [28,30,31] located at amino acid residues 190 to 194 in the putative Mrcasp amino acid sequence. ScanProsite analysis tool revealed that the predicted large subunit domain located at amino acids from 65 to 196 and the small subunit domain located at residues 222 to 318 (Fig.…”

Section: Isolation and Sequence Analysis Of A Novel Mrcasp Cdnamentioning

confidence: 99%

“…Caspase-3 is an important terminal effector caspase in apoptosis and involved in virus-induced apoptosis [29,30]. There are several reports of caspase 3 in shrimp such as banana prawn Penaeus (Fenneropenaeus) merguiensis [28], P. monodon [30] and whiteleg shrimp Penaeus (Litopenaeus) vannamei [31]. Wongprasert et al [20] discovered that caspase-3 activity increased in WSSV-infected P. monodon.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis

Youngcharoen

Senapin

Lertwimol

et al. 2015

Fish & Shellfish Immunology

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Section: Tissue Expression Of Mrcaspsupporting

confidence: 66%

Section: Isolation and Sequence Analysis Of A Novel Mrcasp Cdnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis

Youngcharoen

Senapin

Lertwimol

et al. 2015

Fish & Shellfish Immunology

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“…In Penaeus monodon, the injection of white spot syndrome virus and yellow head virus induces severe apoptotic response in hemocytes, lymphoid organs, and gills (Sahtout et al, 2001;Khanobdee et al, 2002). In Litopenaeus vannamei, the injection of Vibrio alginolyticus leads to significant decline in the total hemocyte counts, accompanied by the apoptosis of hemocytes being detected simultaneously (Chang et al, 2008;Li and Chen, 2008). In our previous study, we demonstrated that E. sinensis experienced drastic decline and rapid recovery in circulating hemocytes after the injection of LPS (Lv et al, 2014).…”

Section: Introductionmentioning

confidence: 91%

“…This process can be triggered by various stimulants, such as virus and bacterium (Sahtout et al, 2001;Chang et al, 2008). In Penaeus monodon, the injection of white spot syndrome virus and yellow head virus induces severe apoptotic response in hemocytes, lymphoid organs, and gills (Sahtout et al, 2001;Khanobdee et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Lyu

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:In the present study, we investigated the possible toxicity mechanism of lipopolysaccharide (LPS) extracted from Gram-negative bacteria in Eriocheir sinensis hemocytes. Apoptotic hemocytes and reactive oxygen species (ROS) production induced by the LPS were monitored by the combination of flow cytometry and microscope observation. It was shown that LPS induced serious damage on the DNA and morphological changes in hemocytes, including cell shrinkage, fracture of nucleus membrane, margination, condensation and fragmentation of chromatin, and formation of apoptotic bodies indicating obvious hemocyte apoptosis. As compared with the control group, the apoptotic cell ratio increased to 30.61% and 39.01% after 1-h exposure and 57.72% and 75.01% after 2-h exposure to 1 and 10 μg/ml LPS, respectively (P<0.05). Significant outburst of ROS production was observed in LPS-treated hemocytes with approximately 176.6% of relative dichlorofluorescein mean fluorescence at 1-h exposure, followed by a drastic decline (P<0.05). These results indicated that LPS would induce oxidative stress on hemocytes from E. sinensis and cause ROS burst, DNA damage, and subsequently apoptosis. The process of ROS-mediated apoptosis might be one of the potential toxicity mechanisms of LPS on crustacean hemocytes.

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Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio alginolyticus and Vibrio fischeri

et al. 2021

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Vibrio alginolyticus and Vibrio (Aliivibrio) fischeri are Gram-negative bacteria found globally in marine environments. During the past decade, studies have shown that certain Gram-negative bacteria, including Vibrio species (cholerae, parahaemolyticus, and vulnificus) are capable of using exogenous polyunsaturated fatty acids (PUFAs) to modify the phospholipids of their membrane. Moreover, exposure to exogenous PUFAs has been shown to affect certain phenotypes that are important factors of virulence. The purpose of this study was to investigate whether V. alginolyticus and V. fischeri are capable of responding to exogenous PUFAs by remodeling their membrane phospholipids and/or altering behaviors associated with virulence. Thinlayer chromatography (TLC) analyses and ultra-performance liquid chromatographyelectrospray ionization mass spectrometry (UPLC/ESI-MS) confirmed incorporation of all PUFAs into membrane phosphatidylglycerol and phosphatidylethanolamine.Several growth phenotypes were identified when individual fatty acids were supplied in minimal media and as sole carbon sources. Interestingly, several PUFAs acids inhibited growth of V. fischeri. Significant alterations to membrane permeability were observed depending on fatty acid supplemented. Strikingly, arachidonic acid (20:4) reduced membrane permeability by approximately 35% in both V. alginolyticus and V. fischeri. Biofilm assays indicated that fatty acid influence was dependent on media composition and temperature. All fatty acids caused decreased swimming motility in V. alginolyticus, while only linoleic acid (18:2) significantly increased swimming motility in V. fischeri. In summary, exogenous fatty acids cause a variety of changes in V. alginolyticus and V. fischeri, thus adding these bacteria to a growing list of Gram-negatives that exhibit versatility in fatty acid utilization and highlighting the potential for environmental PUFAs to influence phenotypes associated with planktonic, beneficial, and pathogenic associations.

show abstract

The effect of Vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp Litopenaeus vannamei

Cited by 61 publications

References 37 publications

Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis

Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio alginolyticus and Vibrio fischeri

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