The effect of ventilation with different concentrations of oxygen upon the synaptic vesicle density in nerve endings of the cat carotid body

S, Morgan; Pallot, D. J.; Willshaw, Peter

doi:10.1016/0306-4522(81)90201-3

Cited by 11 publications

(9 citation statements)

References 16 publications

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“…according to Mills and Slotkin [1975], the release of catecholamines from the cat carotid body during hypoxia is at least partially dependent upon an intact sinus nerve. There is also the question of the function of the efferent pathway de scribed by Morgan et al [ 1981 ], which, in view of the present results, appears redundant.…”

Section: Discussionmentioning

confidence: 70%

Quantitative Studies of the Cat Carotid Body

Pallot¹,

Al-Neamy²,

Blakeman³

1985

Cells Tissues Organs

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The work reported here examines the quantitative ultrastructure of cat carotid body type I cells, and the effects of hypoxia on the cells. On the basis of size and density of electron-dense cored vesicle (EDCV) we were unable to identify more than one major population of type I cells. Prior ventilation of the animals with 10% O₂resulted in an increase in the volume percentage (Vv%) of mitochondria and a decrease in the Vv% of EDCV as compared to the values after ventilation with 100% O₂. The lack of effect of prior denervation of the carotid body on the hypoxic changes suggests that the effects are not mediated via an efferent pathway.

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Section: Discussionmentioning

confidence: 70%

Quantitative Studies of the Cat Carotid Body

Pallot¹,

Al-Neamy²,

Blakeman³

1985

Cells Tissues Organs

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“…Following perfusion fixation the carotid bodies were removed and embedded in Araldite. Ultrathin sections were prepared as previously described [Morgan et al, 1981]. All of the type 1 cell nerve endings which could be positively identitied in single sections were then photographed at an initial magnification of 21 K in either a JEOL 100S or JEOL 100CX electron microscope.…”

Section: Methodsmentioning

confidence: 99%

“…The detailed methodology concerning animal prep aration has been published elsewhere [Morgan et al, 1981). Briefly: anaesthetised animals were prepared with one carotid body with an intact innervation whilst in the contralateral organ the sinus nerve was sectioned.…”

Section: Methodsmentioning

confidence: 99%

“…As most phy siological studies have been performed in the cat, we have examined the nerve endings using morphometric and stereological techniques. In particular, in view of the studies of Morgan et al [1981] demonstrat ing the existence of an efferent effect on type I cell nerve endings, we were particularly interested in whether more than one variety of nerve ending could be distinguished in this species on the basis of membrane specialisation and of differences in synaptic vesicle size and density.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Ultrastructural Studies of the Cat Carotid Body

Dj¹,

Blakeman²

1982

Cells Tissues Organs

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The results of a stereological analysis of cat carotid body type I cells are described. General ultrastructure is described as well as quantitative analysis of the cell organelles. Four types of nerve endings are identified on the basis of their synaptic membrane specialisations. 92.99% symmetrical (unclassifiable), 4.9% pre-synaptic, 1.5% post-synaptic, 0.6% reciprocal. The species difference in comparison to the rat is noted and locations of afferent and efferent terminations are discussed.

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“…After removal and post-fixation in 2% phosphate-buf fered osmium tetroxide, the whole carotid bodies were embedded in Araldite. Sections prepared at 10-to 15-pm intervals were mounted on single slot formvar/carbon-coated grids, stained with methanolic uranyl ace tate and lead citrate and examined in either a JEOL 100S or JEOL 100CX electron microscope [for addi tional details see Morgan et al, 1981].…”

Section: Methodsmentioning

confidence: 99%