The effect of VEGF on the temporal–spatial change of α-tubulin and cortical granules of ovine oocytes matured in vitro

Cao, Xiamin; Zhou, Ping; Luo, Haiwei; Zhao, Yuanyuan; Guo-qing, Shi

doi:10.1016/j.anireprosci.2008.08.013

Cited by 13 publications

(11 citation statements)

References 71 publications

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“…These results are in agreement with that reported by Luo et al () who demonstrated that VEGF enhances bovine IVM, fertilization and early embryo development. Similar effects were observed in ovine and porcine (Luo et al, ; Cao et al, ; Biswas and Hyun, ; Biswas et al, ). Relatively little is known about the biochemical pathway whereby VEGF stimulates the IVM.…”

Section: Discussionsupporting

confidence: 72%

Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

Anchordoquy

Testa

Sirini

et al. 2015

Cell Biology International

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The objective of this study was to investigate the effect of VEGF and Cysteamine during in vitro maturation (IVM) of bovine oocytes on GSH content and developmental competence. For this purpose, experiments were designed to evaluate the effect of 0, 100, 300, and 500 ng/mL VEGF in IVM medium on: GSH content in oocytes and cumulus cells (Exp. 1) and subsequent embryo development (Exp. 2). Also, influence of adding 500 ng/mL VEGF and 100 μM Cysteamine to IVM medium on GSH content in oocytes and cumulus cells (Exp. 3) and oocyte developmental capacity (Exp. 4) were evaluated. Oocytes were matured in: a) Control; b) VEGF 0-3 h; c) Cysteamine 4-24 h; d) VEGF 0-3 h + Cysteamine 4-24 h; and e) VEGF + Cysteamine 24 h. The results showed that: i) VEGF did not alter GSH content in oocytes and cumulus cells; (ii) supplementation of 300 and 500 ng/mL VEGF increased blastocyst yield; (iii) the presence of VEGF + Cysteamine simultaneously during 24 h improved GSH content but not embryo development; and (iv) the presence of VEGF during the first 3 h + Cysteamine from 4 to 24 h increased GSH concentrations and subsequent embryo development. In conclusion, the addition of VEGF and Cysteamine in two sequential steps to maturation medium result in an improvement of cytoplasmic maturation, with a positive impact on oocyte developmental capacity by increasing the efficiency of in vitro blastocyst production. However, the effect was detrimental when both VEGF and Cysteamine were present during 24 of IVM.

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Section: Discussionsupporting

confidence: 72%

Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

Anchordoquy

Testa

Sirini

et al. 2015

Cell Biology International

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“…However, we report, for the first time, that the addition of VEGF-A 165 enhances the progression of meiosis to the MII stage, this rate being 29.4% in preantral follicles grown in vitro. In sheep oocytes grown in vivo, Cao et al (2009) have observed that VEGF-A 165 significantly increases the percentage of oocytes at MII and promotes a normal distribution of α-tubulin and chromosomes in the spindle. This suggests that the exposure of oocytes to VEGF-A 165 improves the organization of the cytoskeleton and that this cellular modification is beneficial to the progression of meiosis.…”

Section: Discussionmentioning

confidence: 98%

Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

et al. 2011

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The aim of this study was to evaluate the effect of vascular endothelial growth factor-A(165) (VEGF-A(165)) on the in vitro development of goat secondary preantral follicles. Preantral follicles (≥150 μm in diameter) were isolated from the ovaries of adult mixed-breed goats and individually cultured for 18 days in αMEM in the absence (control) or presence of VEGF-A(165) at concentrations of 10 ng/ml (VEGF10) and 100 ng/ml (VEGF100). Analyses of follicular survival, diameter, antrum formation and rate of daily growth were performed every 6 days. At the end of the culture period, morphologically normal oocytes (≥110 μm in diameter) were taken for in vitro maturation (IVM). The results demonstrated that all follicles presented oocytes and granulosa cells that were morphologically normal and after labeling with calcein-AM, high rates of oocyte viability were observed in all treatments. The follicular diameter and the growth rate achieved in the presence of VEGF10 were higher than those of the control. Both treatments with VEGF-A(165) showed higher rates of oocyte recovery for IVM when compared with the control. Moreover, only the addition of VEGF-A(165) permitted oocytes grown in vitro to reach metaphase II. Thus, the addition of VEGF-A(165) to the culture medium improves the development of goat preantral follicles cultured in vitro, allowing the production of mature oocytes.

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“…The addition of oestrus serum also supported high maturation rates in IVM of ewe oocytes (Rao et al, 2002;Succu et al, 2007). In addition, the research into supplementing recombinant hormones such as leptin, insulin-like growth factor, and vascular endothelial growth factor in IVM system has served to improve development of in vitro maturation in several species (Moreira et al, 2002;Boelhauve et al, 2005;Cao et al, 2009;Kim et al, 2010). However, developmental competence of in vitro matured oocytes from prepubertal animals has been reported to be lower than oocytes derived from adult animals (Pinyopummintr & Bavister, 1995;O'Brien et al, 1996;Ledda et al, 1997;Gandolfi et al, 2000;Leoni et al, 2007).…”

Section: Discussionmentioning

confidence: 98%

“…However, developmental competence of in vitro matured oocytes from prepubertal animals has been reported to be lower than oocytes derived from adult animals (Pinyopummintr & Bavister, 1995;O'Brien et al, 1996;Ledda et al, 1997;Gandolfi et al, 2000;Leoni et al, 2007). In addition, the researches supplementing recombinant hormones such as leptin, insulin-like growth factor, and vascular endothelial growth factor in IVM system have been used to improve development of in vitro maturation in several species (Moreira et al, 2002;Boelhauve et al, 2005;Cao et al, 2009;Kim et al, 2010). In addition, the research into supplementing recombinant hormones such as leptin, insulin-like growth factor, and vascular endothelial growth factor in IVM system has served to improve development of in vitro maturation in several species (Moreira et al, 2002;Boelhauve et al, 2005;Cao et al, 2009;Kim et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effect of lanosterol on thein vitromaturation in semi-defined culture system of prepubertal ewe oocytes

Marco‐Jiménez

Vicente

Viudes-de-Castro

2011

Zygote

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The choice of medium and supplements can affect meiotic regulation and may have an impact on the regulation of mammalian oocyte growth and embryonic cell function. The aim of the present study was to assess the effects of oxygen concentration and endogenous lanosterol on the in vitro maturation (IVM) media without serum and based on recombinant human chorionic gonadotrophin in prepubertal ewe oocytes. Firstly, the effect of varying oxygen concentrations (5% and 20%) during IVM in TCM-199 supplemented (4 mg/ml bovine serum albumin (BSA), 100 μM cysteamine, 0.3 mM sodium pyruvate, 0.1 UI/ml recombinant follicle-stimulating hormone (r-FSH; Gonal-F® 75 UI, Serono, Italy), 0.1 UI/ml recombinant leuteinizing hormone (r-LH; Lhadi® 75 UI, Serono, Italy) and 1 μg/ml estradiol-17β) on subsequent nuclear maturation of oocytes examined under ultraviolet light following staining with bisbenzimide (Hoechst 33342) was investigated. Secondly, two concentrations of lanosterol (0, 10 and 50 μM) were added to the IVM medium. Nuclear maturation of oocytes was examined as previously. Lipid content in oocytes, an important indicator of cytoplasmic maturity, was also measured using Nile red fluorescent stain. The results showed that low oxygen concentration affected the nuclear maturation. Similarly, a significantly higher rate of meiosis resumption was observed with 10 μM (72.3%) of lanosterol compared with the control (51.8%) or 50 μM of lanosterol (59.4%). A significantly higher content of lipids was also observed with 10 and 50 μM of lanosterol (7.3 ± 0.2 × 10(6) and 7.4 ± 0.2 × 10(6) arbitrary units of fluorescence) compared with the control (6.7 ± 0.2 × 10(6) arbitrary units of fluorescence). The results indicate that 10 μM lanosterol during IVM in medium without serum and based on recombinant human chorionic gonadotrophin has a positive effect on maturation of prepubertal ewe oocytes.

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The effect of VEGF on the temporal–spatial change of α-tubulin and cortical granules of ovine oocytes matured in vitro

Cited by 13 publications

References 71 publications

Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development

Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

Effect of lanosterol on thein vitromaturation in semi-defined culture system of prepubertal ewe oocytes

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