The effect of VEGF functionalization of titanium on endothelial cells in vitro

Poh, Chye Khoon; Shi, Zhijun; Lim, Tze-Peng; Neoh, K. G.; Wang, Wilson

doi:10.1016/j.biomaterials.2009.11.042

Cited by 214 publications

(168 citation statements)

References 54 publications

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“…27 Briefly, ice-cold matrigel (BD Biosciences) was pipetted into a 96-well plate and allowed to solidify at 37°C for 1 h. HUVECs were then plated onto the matrigel and incubated with 50 μg/mL HAPs for 8 h. The capillary-like tube formation was viewed with …”

Section: Tube Formation Assaymentioning

confidence: 99%

Interaction of hydroxyapatite nanoparticles with endothelial cells: internalization and inhibition of angiogenesis in vitro through the PI3K/Akt pathway

Shi¹,

Zhou²,

Huang³

et al. 2017

IJN

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Nano-hydroxyapatite (nano-HAP) has been proposed as a better candidate for bone tissue engineering; however, the interactions of nano-HAP with endothelial cells are currently unclear. In this study, HAP nanoparticles (HANPs; 20 nm np20 and 80 nm np80) and micro-sized HAP particles (m-HAP; 12 μm) were employed to explore and characterize cellular internalization, subcellular distribution, effects of HANPs on endothelial cell function and underlying mechanisms using human umbilical vein endothelial cells (HUVECs) as an in vitro model. It was found that HANPs were able to accumulate in the cytoplasm, and both adhesion and uptake of the HANPs followed a function of time; compared to np80, more np20 had been uptaken at the end of the observation period. HANPs were mainly uptaken via clathrin- and caveolin-mediated endocytosis, while macropinocytosis was the main pathway for m-HAP uptake. Unexpectedly, exposure to HANPs suppressed the angiogenic ability of HUVECs in terms of cell viability, cell cycle, apoptosis response, migration and capillary-like tube formation. Strikingly, HANPs reduced the synthesis of nitric oxide (NO) in HUVECs, which was associated with the inhibition of phosphatidylinositol 3-kinase (PI3K) and phosphorylation of eNOS. These findings provide additional insights into specific biological responses as HANPs interface with endothelial cells.

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Section: Tube Formation Assaymentioning

confidence: 99%

Interaction of hydroxyapatite nanoparticles with endothelial cells: internalization and inhibition of angiogenesis in vitro through the PI3K/Akt pathway

Shi¹,

Zhou²,

Huang³

et al. 2017

IJN

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“…Dopamine, a mussel-inspired molecule, can undergo self-polymerization and adhere onto almost any solid surface without surface pre-treatments. More importantly, polydopamine ( pDA) coating can function as an anchor to graft secondary functional biopolymers by thiols and amines via Michael addition or Schiff base reactions [27][28][29]. In this study, we present a novel CS-decorated Ti implant with antibacterial activity through adhesive dopamine for the first time.…”

Section: Introductionmentioning

confidence: 99%

“…These substrates were polished with a series of SiC abrasive papers (400, 1000, 1500, 2000 grit), cleaned ultrasonically for 20 min in baths of acetone, anhydrous ethanol and DI water, respectively, and then dried at 508C overnight. pDA was anchored to the surface of Ti discs as described elsewhere [27,28], via immersion of the substrates in a 2 mg ml 21 solution of dopamine (10 mM Tris-HCl buffer, pH ¼ 8.5), and gently shaken overnight in the dark. After this process, these Ti substrates were then rinsed with DI water to remove the unattached dopamine molecules and dried in a stream of nitrogen.…”

Section: Immobilization Of Cefotaxime Sodium On the Surface Of Titaniummentioning

confidence: 99%

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Zhou

Wang

et al. 2014

J. R. Soc. Interface.

114

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Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.

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“…Nerve growth factor (NGF) immobilised onto nanofibrous polycaprolactone-polyethylene glycol (PCL/PCL-PEG) scaffolds also induced the neural differentiation of MSCs [76] . Vascular endothelial growth factor (VEGF) has also demonstrated to induce differentiation of mouse ESCs and human MSCs into endothelial cells [77][78][79] . The modification of materials with specific chemical functional groups is a common strategy to induce specific stem cell behaviour [93] .…”

Section: A B Cmentioning

confidence: 99%

“…Curran et al [66] Peptide sequence Alginate Osteopontin peptide Increase in hMSC osteogenic markers Lee et al [67] HA-PLG Osteocalcin peptide Increase hMSC osteogenic markers Lee et al [68] PLGA BMP-2 peptide Increase rMSC ALP expression in osteogenic medium and promotion of ectopic bone formation in vivo Lin et al [15] RGD BCP/PLA hMSC osteogenic differentiation Shin et al [70] Molecule PLGA BMP-2 hMSC osteogenic differentiation Ko et al [71] PLLA BMP-2 hMSC osteogenic differentiation Beazley et al [16] Collagen-PLGA hybrid Collagen-binding domain derived from fibronectin (CBD-BMP4) hMSC osteogenic differentiation Lu et al [72] Methacrylamide chitosan hydrogel coated glass substrates Laminin and collagen Supported hNSC differentiation Wilkinson et al [73] PMMA-g-PEG EGF MSC osteogenic differentiation Platt et al [74] Agarose PDGF-AA MSC neural differentiation Aizawa et al [75] PCL/PCL-PEG NGF MSC neural differentiation Cho et al [76] Chitosan/collagen Ⅳ VEGF Endothelial differentiation Chiang et al [77] , Poh et al [78] , Rahman et al [79] hESC: Human embryonic stem cell; hMSC: Human mesenchymal stem cell; rMSCs: Rat mesenchymal stem cell; hNSC: Human neural stem cell; PEG: Polyethylene glycol; HA-PLG: Hydroxyapatite (HA)/poly(lactic-co-glycolic acid); BMP: Bone morphogenetic protein; PLGA: Poly(lactic-co -g lycolic acid); PLA: Poly(lactic acid); PLLA: Poly(L-lactide); PCL: Polycaprolactone; PMMA-g-PEG: Poly(methyl methacrylate)-graft-poly(ethylene glycol; BCP: Biphasic calcium phosphate; EGF: Epidermal growth factor; NGF: Nerve growth factor; PDGF-AA: Platelet-derived Growth Factor AA; VEGF: Vascular endothelial growth factor.…”

Section: Neuronalmentioning

confidence: 99%

Control of stem cell fate by engineering their micro and nanoenvironment

Griffin

Butler

Seifalian

et al. 2015

WJSC

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Stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix (ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine.

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The effect of VEGF functionalization of titanium on endothelial cells in vitro

Cited by 214 publications

References 54 publications

Interaction of hydroxyapatite nanoparticles with endothelial cells: internalization and inhibition of angiogenesis in vitro through the PI3K/Akt pathway

Interaction of hydroxyapatite nanoparticles with endothelial cells: internalization and inhibition of angiogenesis in vitro through the PI3K/Akt pathway

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Control of stem cell fate by engineering their micro and nanoenvironment

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