The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching

Anderson, Seth C.; Siegman, Shayne N.; Segura, Tatiana

doi:10.1016/j.biomaterials.2011.06.027

Cited by 77 publications

(67 citation statements)

References 36 publications

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“…The enhanced HUVEC activities and angiogenic effects of the RGD, which was derived from laminin in the native ECM, and VEGF, which is a potent known angiogenic factor, have been previously reported. 4,12 In the present study, in situ RGD conjugated GPT (RGD−GPT) hydrogels and VEGF loaded GPT (GPT/VEGF) hydrogels were used as positive control groups to confirm the superior effect of SV−Y peptide conjugation on the stimulation of HUVEC activities. In these experiments, RGD−GPT hydrogels was prepared by in situ conjugation of the RGD−Y peptide to the GPT hydrogels via the enzyme-mediated reaction and the same concentration of RGD−Y (2 mM) was used.…”

Section: Scheme 2 Schematic Representation Of the In Situ Sv−y Conjumentioning

confidence: 99%

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

et al. 2012

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Tissue engineering therapies require biocompatible and bioactive biomaterials that are capable of encouraging an angiogenic response for effective tissue regeneration. In this study, a SVVYGLR peptide, which functions as a potent angiogenic factor, was conjugated into injectable gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogels in situ to enhance endothelial cell activities and neo-vascularization. SVVYGLRGGY (SV-Y) conjugated GPT (SV-GPT) hydrogels were formed in situ via enzyme-mediated reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). The physico-chemical properties were characterized and could be controlled depending on the feed peptide and H(2)O(2) concentration. The concentration of conjugated peptide ranged from 0.37 to 0.81 μmol/mL, and the elastic moduli (G') of the hydrogels were 600-4900 Pa. In vitro cell studies using human umbilical vein endothelial cells (HUVECs) and in vivo subcutaneous injection studies were performed to confirm the effect of the SVVYGLR peptide on HUVEC activity and neo-vascularization. Obtained results demonstrated that the in situ conjugation of SVVYGLR sequences into phenol residues of GPT hydrogels enhanced the activity of HUVECs in vitro and stimulated the formation of new blood vessels in the hydrogel matrices in vivo. From the results, we suggest that in situ conjugation of SV-Y to GPT hydrogels via the enzymatic reaction may be an efficient tool to prepare injectable bioactive hydrogels that can enhance endothelial cell activities and promoting angiogenesis for tissue regeneration.

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Section: Scheme 2 Schematic Representation Of the In Situ Sv−y Conjumentioning

confidence: 99%

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

et al. 2012

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“…3 Briefly, HUVECs were starved in serum-free media for 6 h before exposure to n(VEGF) or VEGF for different amounts of time. Treated cells were lysed in mRIPA lysis buffer (supplemented with fresh aprotinin, leupeptin and PMSF) and collected for supernatants.…”

Section: Methodsmentioning

confidence: 99%

Cell-Demanded VEGF Release via Nanocapsules Elicits Different Receptor Activation Dynamics and Enhanced Angiogenesis

Zhu

Segura

2016

Ann Biomed Eng

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Although the delivery of vascular endothelial growth factor (VEGF) with extended release profiles has consistently shown beneficial therapeutic effects compared with bolus delivery, it remains unclear if the reason is solely due to the physical availability and the reduced degradation of the protein. Here we studied the activation of VEGF receptor 2 (VR-2) by sustained released VEGF compared with bolus delivered VEGF to unveil that sustained delivery system alters the dynamics of receptor activation and affects the actions of cells between sprouting and proliferation. We utilized a protein nanocapsule delivery strategy that releases VEGF as mediated by extracellular proteases. These protein nanocapsules were synthesized through an aqueous assembly of a nanogel-peptide shell around the protein, leading to one to two proteins encapsulated per nanocapsule. Receptor activation studies revealed differential dynamics of receptor activation for slowly released VEGF compared with bolus delivered VEGF. As expected sustained released VEGF via nanocapsules resulted in enhanced vascular sprouting in vitro and in vivo. These studies demonstrate the physical presentation of VEGF, in this case of a slow release with time, can affect its molecular mechanism of actions and cause alterations in cellular responses and therapeutic outcomes.

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“…Many angiogenic factors such as vascular endothelial growth factor (VEGF) (des Anderson et al 2011), fibroblast growth factor (FGF) Zhu et al 2008), TGF-β ) and angiopoietin 1 (Ang1) (Chiu and Radisic 2010) have been used for promoting the vascularization of scaffolds. VEGF has gained considerable attention due to its central role in physiology and neovascularization of endothelial cells.…”

Section: Delivery Of Angiogenic Factorsmentioning

confidence: 99%

Vascularization in the Bone Repair

Zhou¹,

Dong²

2012

Osteogenesis

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The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching

Cited by 77 publications

References 36 publications

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

Cell-Demanded VEGF Release via Nanocapsules Elicits Different Receptor Activation Dynamics and Enhanced Angiogenesis

Vascularization in the Bone Repair

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