The effect of various morphine weaning regimens on the sequelae of opioid tolerance involving physical dependency, anxiety and hippocampus cell neurodegeneration in rats

Motaghinejad, Majid; Karimian, Seyed Morteza; Motaghinejad, Ozra; Shabab, Behnaz; Asadighaleni, Majid; Fatima, Sulail

doi:10.1111/fcp.12121

Cited by 39 publications

(62 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The homogenized cell solutions were analyzed for measurement of oxidative stress and apoptosis factors (Motaghinejad et al, 2015a, Motaghinejad et al, 2015b, Motaghinejad and Motevalian, 2016.…”

Section: 5 Mitochondrial Preparationmentioning

confidence: 99%

“…The quantity of this compound was calculated using an extension coefficient of 165 mM -1 cm -1 at 530 nm. Results were expressed as nmol/mg protein (Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b, Serteser et al, 2002.…”

Section: 51 Determination Of Lipid Peroxidationmentioning

confidence: 99%

“…The reduced and oxidized forms of glutathione (GSH and GSSG, respectively) were measured using two different kits: Bioxytech GPx-340 and Bioxytech GR-340 (Oxis Research, USA) and the results were reported as nmol/mg protein (Capela et al, 2007, Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b was added to each well. The further incubation was carried out for 1 h at room temperature.…”

Section: 52 Determination Of Gsh and Gssg Contentmentioning

confidence: 99%

“…The detection limit of the assay was determined to be 4.3 pg/ml. Results were expressed as ng IL-1β/ml or TNF-α/ml (Ji et al , 2015, Kuloglu et al , 2002, Motaghinejad, Karimian, 2015b. …”

Section: 52 Determination Of Gsh and Gssg Contentmentioning

confidence: 99%

“…The absorbance was detected at 450 nm and the findings were expressed as U/ml/mg protein(Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b.<H3>2.5.4 Determination of glutathione peroxidase (GPx) activityThe activity of GPx was measured by Bioxytec GPx-340 kits (Oxis Research, USA). Results were reported as mU/mg protein(Hsieh et al, 2015, Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b.<H3>2.5.5 IL-1β and TNF-α measurementConcentrations of IL-1β and TNF-α in supernatant of hippocampal cells were measured using a commercially available ELISA kit (Genzyme Diagnostics, Cambridge, U.S.A.). Briefly, 96 well microtitre plates (Nunc) were coated with sheep anti-rat IL-1β and TNF-α polyclonal antibodies (2 mg/ml in bicarbonate coating buffer; 0.1 M NaHCO3, 0.1 M NaCl, pH 8.2, for 20 h at 48 °C), then washed three times with washing buffer (0.5 M NaCl, 2.5 mM NaH2PO4, 7.5 mM Na2HPO4, 0.1% Tween 20, pH 7.2).…”

mentioning

confidence: 99%

See 4 more Smart Citations

The neuroprotective effect of lithium against high dose methylphenidate: Possible role of BDNF

et al. 2016

Self Cite

View full text Add to dashboard Cite

Section: 5 Mitochondrial Preparationmentioning

confidence: 99%

Section: 51 Determination Of Lipid Peroxidationmentioning

confidence: 99%

Section: 52 Determination Of Gsh and Gssg Contentmentioning

confidence: 99%

Section: 52 Determination Of Gsh and Gssg Contentmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The neuroprotective effect of lithium against high dose methylphenidate: Possible role of BDNF

et al. 2016

Self Cite

View full text Add to dashboard Cite

Curcumin‐ZnO conjugated nanoparticles confer neuroprotection against ketamine‐induced neurotoxicity

Mobinhosseini,

Salehirad,

Wallace Hayes

et al. 2023

J Biochem & Molecular Tox

Self Cite

View full text Add to dashboard Cite

BackgroundNanotechnology and its application to manipulate herbal compounds to design new neuroprotective agents to manage neurotoxicity has recently increased. Cur‐ZnO conjugated nanoparticles were synthesized and used in an experimental model of ketamine–induced neurotoxicity.MethodsCur‐ZnO conjugated nanoparticles were chemically characterized, and the average crystalline size was determined. Forty‐nine adult mice were divided into seven groups of seven animals each. Normal saline was given to control mice (group 1). Ketamine (25 mg/kg) was given to a second group. A third group of mice was given ketamine (25 mg/kg) in combination with curcumin (40 mg/kg), while mice in groups 4, 5, and 6 received ketamine (25 mg/kg) plus Cur‐ZnO nanoparticles (10, 20, and 40 mg/kg). Group 7 received only ZnO (5 mg/kg). All doses were ip for 14 days. Hippocampal mitochondrial quadruple complex enzymes, oxidative stress, inflammation, and apoptotic characteristics were assessed.ResultsCur‐ZnO nanoparticles and curcumin decreased lipid peroxidation, GSSG content, IL‐1β, TNF‐α, and Bax levels while increasing GSH and antioxidant enzymes like GPx, GR, and SOD while increasing Bcl‐2 level and mitochondrial quadruple complex enzymes in ketamine treatment groups.ConclusionThe neuroprotective properties of Cur‐ZnO nanoparticles were efficient in preventing ketamine‐induced neurotoxicity in the mouse brain. The nanoparticle form of curcumin (Cur‐ZnO) required lower doses to produce neuroprotective effects against ketamine‐induced toxicity than conventional curcumin.

show abstract

Neuroprotective effects of various doses of topiramate against methylphenidate-induced oxidative stress and inflammation in isolated rat amygdala: the possible role of CREB/BDNF signaling pathway

et al. 2016

View full text Add to dashboard Cite

Methylphenidate (MPH) abuse damages brain cells. The neuroprotective effects of topiramate (TPM) have been reported previously, but its exact mechanism of action still remains unclear. This study investigated the in vivo role of various doses of TPM in the protection of rat amygdala cells against methylphenidate-induced oxidative stress and inflammation. Seventy adult male rats were divided into seven groups. Groups 1 and 2 received normal saline (0.7 ml/rat) and MPH (10 mg/kg), respectively, for 21 days. Groups 3, 4, 5, 6, and 7 were concurrently treated with MPH (10 mg/kg) and TPM (10, 30, 50, 70, and 100 mg/kg), respectively, for 21 days. elevated plus maze (EPM) was used to assess motor activity disturbances. In addition, oxidative, antioxidantand inflammatory factors and CREB, Ak1, CAMK4, MAPK3, PKA, BDNF, and c FOS gene levels were measured by RT-PCR, and also, CREB and BDNF protein levels were measured by WB in isolated amygdalae. MPH significantly disturbed motor activity and TPM (70 and 100 mg/kg) neutralized its effects. MPH significantly increased lipid peroxidation, mitochondrial GSSG levels and IL-1β and TNF-α level and CAMK4 gene expression in isolated amygdala cells. In contrast, superoxide dismutase, glutathione peroxidase, and glutathione reductase activities and CREB, BDNF Ak1, MAPK3, PKA, BDNF, and c FOS expression significantly decreased. The various doses of TPM attenuated these effects of MPH. It seems that TPM can be used as a neuroprotective agent and is a good candidate against MPH-induced neurodegeneration.

show abstract

The effect of various morphine weaning regimens on the sequelae of opioid tolerance involving physical dependency, anxiety and hippocampus cell neurodegeneration in rats

Cited by 39 publications

References 45 publications

The neuroprotective effect of lithium against high dose methylphenidate: Possible role of BDNF

The neuroprotective effect of lithium against high dose methylphenidate: Possible role of BDNF

Curcumin‐ZnO conjugated nanoparticles confer neuroprotection against ketamine‐induced neurotoxicity

Neuroprotective effects of various doses of topiramate against methylphenidate-induced oxidative stress and inflammation in isolated rat amygdala: the possible role of CREB/BDNF signaling pathway

Contact Info

Product

Resources

About