The effect of vaginal lubricants on Neisseria gonorrhoeae

Singh, Balwant; Cutler, John C.

doi:10.1016/0002-9378(76)90551-2

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…A previous study by Singh et al in 1976 5 showed considerable inhibition when using a lubricant that is no longer available and therefore recommended that lubricants should be avoided. However, they did not state whether the lubricant contained any bactericidal agent, and their results from the same study but using K-Y jelly, which does not contain a bactericide, were very encouraging.…”

Section: Discussionmentioning

confidence: 99%

Plastic specula: can we ease the passage?

Kozakis

Vuddamalay²,

Munday³

2006

Sexually Transmitted Infections

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Background: For many years, genitourinary physicians have taught that specula should be used without lubrication other than water, as it was assumed that gel would interfere with the processing of samples, but there seems little evidence to support this. Many clinics are now using plastic specula to avoid reusable instruments, and one of the commonest problems with such specula is increased friction. Methods: We looked at the effect of Aquagel on the culture of different dilutions of Neisseria gonorrhoeae on three standard laboratory media. The effect of Aquagel on the chlamydial strand displacement assay (SDA) test was also assessed by mixing different amounts of Aquagel with the positive and negative control and processing in the usual way.Results: There was found to be no inhibition of culture following emulsification of N gonorrhoeae in Aquagel at any concentration. All the results for the chlamydial SDA test were satisfactory following mixing with Aquagel. Conclusion: We think that the clinician should now be more confident that if a difficult examination requires the use of a lubricant, the test results will not be compromised.

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Section: Discussionmentioning

confidence: 99%

Plastic specula: can we ease the passage?

Kozakis

Vuddamalay²,

Munday³

2006

Sexually Transmitted Infections

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“…Samples were then diluted in 10-fold dilutions in sterile physiological saline and the number of surviving bacteria determined on chocolate agar plates by the time exposure method described by Singh and Cutler. 11 Control samples without gel formulations were tested in the same way. The titres (log 10 CFU) of gel-bacteria mixtures were subtracted from the titre of the control mixture and the diVerence used as a measure of the microbicidal activities of the gels.…”

Section: Assay Of Microbicidal Activity Of Hydrogels Against N Gonorrmentioning

confidence: 99%

Hydrogels containing monocaprin have potent microbicidal activities against sexually transmitted viruses and bacteria in vitro

Thormar

Bergsson

Gunnarsson

et al. 1999

Sexually Transmitted Infections

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Objective: To investigate the in vitro microbicidal and cytocidal potency of monocaprin dissolved in pharmaceutical hydrogel formulations and to evaluate their potential use as vaginal microbicides against sexually transmitted pathogens such as herpes simplex virus type 2 (HSV-2), human immunodeficiency virus type 1 (HIV-1), Chlamydia trachomatis, and Neisseria gonorrhoeae. Methods: Gel formulations were mixed with equal volumes of virus/bacteria suspensions in culture medium and incubated for 1 and 5 minutes. The reduction in virus/bacteria titre was used as a measure of microbicidal activity. Similarly, gels were mixed with human semen to study their eVect on leucocytes. The toxicity of the gels was tested in rabbits by the standard vaginal irritation test. Results: Gels containing 20 mM of monocaprin caused a greater than 100 000-fold inactivation of HSV-2 and Neisseria in 1 minute and of Chlamydia in 5 minutes. Similarly, the gels caused a greater than 10 000-fold inactivation of HIV-1 in semen in 1 minute. They caused more than a 10 000-fold reduction in the number of viable leucocytes in semen in 1 minute. No toxic eVect on the vaginal mucosa of rabbits was observed after daily exposure for 10 days. Conclusions: Hydrogels containing monocaprin are potent inactivators of sexually transmitted viruses and bacteria in vitro. This simple lipid seems to be a feasible choice as a mucosal microbicide for prevention of sexually transmitted infections. It is a natural compound found in certain foodstuVs such as milk and is therefore unlikely to cause harmful side eVects in the concentrations used. (Sex Transm Inf 1999;75:181-185)

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The sexually transmissible diseases

Fumara¹

1978

Disease-a-Month

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The effect of vaginal lubricants on Neisseria gonorrhoeae

Cited by 5 publications

References 4 publications

Plastic specula: can we ease the passage?

Plastic specula: can we ease the passage?

Hydrogels containing monocaprin have potent microbicidal activities against sexually transmitted viruses and bacteria in vitro

The sexually transmissible diseases

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