The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment

Sztatelman, Olga; Grzyb, Joanna; Gabryś, Halina; Banaś, Agnieszka Katarzyna

doi:10.1186/s12870-015-0667-2

Cited by 59 publications

(48 citation statements)

References 78 publications

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“…66 Furthermore, the UV-B-dependent formation of dimers between adjacent pyrimidines in DNA strands may be both mutagenic and genotoxic due to blocking the progress of DNA polymerase. 67 As a consequence, the exposure of plants to high levels of UV can lead to cell death dependent on ROS signaling. 68 Suchar & Robberecht 69 found out that repair mechanisms could not solely prevent the UV-B radiation interference with the cell division; resulting in significant reductions in leaf growth and development.…”

Section: -63mentioning

confidence: 99%

Interaction of cytokinins with UV-B (280 -315nm) on the expansion growth of cucumber cotyledons

Kataria¹,

Kn²

2018

HIJ

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The negative effect on plant responses are expected due to increased amounts of ultraviolet-B (UV-B) radiation reaching on the Earth's surface by stratospheric ozone depletion. Cytokinins (CKs) are essential phytohormones in plant growth and development. Perusal of relevant literature reveals that CKs mitigate the oxidative damage caused by abiotic stresses like salt, drought, high temperature and heavy metal due to their antioxidant effects. However, very few reports are available on the interaction of CKs with UV-B stress on the expansion growth of the cucumber cotyledons. CKs induced the expansion growth of the excised cucumber cotyledons in the darkness. CKs induced expansion growth of cucumber cotyledons in darkness was inhibited by supplemental UV-B. UV-B radiation enhanced the level of oxyradicals like superoxide and hydroxide radicals in the excised cucumber cotyledons, which was evident by EPR spectroscopy. CKs like Zeatin, TDZ, BAP and FAP reduced the level of oxyradicals produced in the dark grown cucumber cotyledons, while promoting the expansion growth of the cotyledons. Production of oxyradical in UV-B exposed cotyledons showed that these oxyradicals might partially account for inhibition of expansion growth. Since considerable amount of oxyradicals were quenched by CKs at higher concentrations (10 and 20 µg/ml) it partially restores the inhibition of expansion growth caused by UV-B stress. It indicates that overproduction of oxyradicals by UV-B could not be entirely responsible for the inhibition of CKs induced expansion growth of cotyledons; which might be caused by some other physiological changes caused by UV-B irradiation in addition to the production of oxyradicals. UV-B is also known to inactivate protein inhibitor of peroxidase in cucumber cotyledons. This may reduce the antioxidants defence and enhance the damaging effects of oxyradicals for the growth of cotyledons.

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Section: -63mentioning

confidence: 99%

Interaction of cytokinins with UV-B (280 -315nm) on the expansion growth of cucumber cotyledons

Kataria¹,

Kn²

2018

HIJ

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“…Supernatants from both extractions were pooled and adjusted to known volume with methanol. Pigment separation, detection and quantitative analysis were performed according to (Sztatelman et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…Real-time PCR was performed using SYBR Green JumpStart Taq ReadyMix (Sigma Aldrich) and a Rotor-Gene 6000 thermal cycler (Corbett Research, Australia) according to (Łabuz et al, 2012), with the following modifications: reactions were run in triplicates, and UBC, UBQ10 and SAND were used as the reference genes. Primer sequences and annealing temperatures were based on (Sztatelman et al, 2015) and are listed in Table S1 (at https://ojs.ptbioch.edu. pl/index.php/abp/).…”

Section: Methodsmentioning

confidence: 99%

“…This model shares many common features with natural, age-dependent senescence, including leaf yellowing, chlorophyll degradation and induction of senescence associated genes (SAGs) (van der Graaff et al, 2006). To assess the progress of senescence we used several well-established markers, such as photosynthetic efficiency and photosynthetic pigment content in the leaves (Weaver & Amasino, 2001;Sztatelman et al, 2015). We also assessed expression of several genes involved in senescence (SENESCENCE AS-SOCIATED GENES -SAG13, SAG12 and SENES-CENCE1 -SEN1), photosynthesis (RIBULOSE BI-SPHOSPHATE CARBOXYLASE SMALL CHAIN 1A -RbcS1A and CHLOROPHYLL A/B BINDING PROTEINS -CABs, including CAB1 and CAB2) and anthocyanin biosynthesis (PHENYLALANINE AM-MONIA-LYASE 1 -PAL1).…”

Section: Introductionmentioning

confidence: 99%

“…We also assessed expression of several genes involved in senescence (SENESCENCE AS-SOCIATED GENES -SAG13, SAG12 and SENES-CENCE1 -SEN1), photosynthesis (RIBULOSE BI-SPHOSPHATE CARBOXYLASE SMALL CHAIN 1A -RbcS1A and CHLOROPHYLL A/B BINDING PROTEINS -CABs, including CAB1 and CAB2) and anthocyanin biosynthesis (PHENYLALANINE AM-MONIA-LYASE 1 -PAL1). All genes selected for investigation in this study have been previously used in our work on darkening of detached Arabidopsis leaves (Sztatelman et al, 2015. )…”

Section: Introductionmentioning

confidence: 99%

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A role for GLABRA1 in dark-induced senescence

et al. 2019

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The GLABRA (GL1) gene, belonging to the transcription factor-encoding myb gene family, is responsible for trichome formation in Arabidopsis thaliana (L.) Heynh. The leaves and stems of glabra1 mutant plants are devoid of trichomes. Having an easily observable phenotype, the gl1 mutation was one of the first markers established for genetic mapping of Arabidopsis thaliana. Since then, the GL1 gene has been assigned roles in other processes, also related to leaf structure. In this study we present some previously undescribed effects of the gl1 mutation on dark-induced senescence. This process was induced by covering selected mature leaves of Columbia wild-type and gl1 Arabidopsis with black paper for 4 days, while the plants remained growing in a normal photoperiod. While no visible differences in the external symptoms of senescence could be observed in the darkened leaves, the expression of senescence-associated genes was significantly lower in gl1 plants as compared to the wild type. The darkening of leaves led to a decrease in photosynthetic activity and the expression of photosynthesis-associated genes, in comparison to the control leaves. This effect was much less pronounced in gl1 than in the wild type plants. Therefore, gl1 plants seem to be less susceptible to dark-induced aging, suggesting a possible role for the GL1 gene in controlling the onset and progress of senescence. This result is also of practical importance, since gl1 is the genetic background of many other mutants. It may therefore be advisable to revise some of the results obtained with such mutants in light of findings presented here.

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